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Measurement of M. luteus endonuclease-sensitive lesions by alkaline elution

Journal Article:

Abstract

The UV-endonuclease approach to detect DNA damage has been combined with the alkaline elution technique with a resultant marked increase in sensitivity compared to the conventional method using alkaline sedimentation. DNA from UV-irradiated cells was digested on an inert filter with an extract from Micrococcus luteus and then analyzed by alkaline elution. Endonuclease-sensitive sites (endo-sites) were measured after doses of 0.08-0.7 Jm/sup -2/ of UV-radiation. An estimate of endo-site production with UV radiation, 0.27 endo-sites/10/sup 8/ daltons of DNA/0.1 Jm/sup -2/, was similar to that usually seen at higher doses by others. With repair incubation, approx. 50% of the endo-sites were removed in 4 h by normal human fibroblasts after 0.2 or 0.4 Jm/sup -2/, no appreciable repair was seen in xeroderma pigmentosum fibroblasts from complementation group A after 24 h of repair incubation. No photoreaction of UV damage due to 0.4 Jm/sup -2/ was detected in normal human fibroblasts.
Authors:
Fornace, Jr, A J [1] 
  1. National Cancer Inst., Bethesda, MD (USA). Lab. for Experimental Pathology
Publication Date:
Jan 01, 1982
Product Type:
Journal Article
Reference Number:
AIX-14-717255; EDB-83-052501
Resource Relation:
Journal Name: Mutat. Res.; (Netherlands); Journal Volume: 94
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; RADIOSENSITIVITY; BIOLOGICAL REPAIR; COMPARATIVE EVALUATIONS; FIBROBLASTS; GENETIC RADIATION EFFECTS; MAN; MICROCOCCUS LUTEUS; STRAND BREAKS; TUMOR CELLS; ULTRAVIOLET RADIATION; ANIMAL CELLS; ANIMALS; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; GENETIC EFFECTS; MAMMALS; MICROCOCCUS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PRIMATES; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; SOMATIC CELLS; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987)
OSTI ID:
6764565
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: MUREA
Submitting Site:
HEDB
Size:
Pages: 263-276
Announcement Date:

Journal Article:

Citation Formats

Fornace, Jr, A J. Measurement of M. luteus endonuclease-sensitive lesions by alkaline elution. Netherlands: N. p., 1982. Web.
Fornace, Jr, A J. Measurement of M. luteus endonuclease-sensitive lesions by alkaline elution. Netherlands.
Fornace, Jr, A J. 1982. "Measurement of M. luteus endonuclease-sensitive lesions by alkaline elution." Netherlands.
@misc{etde_6764565,
title = {Measurement of M. luteus endonuclease-sensitive lesions by alkaline elution}
author = {Fornace, Jr, A J}
abstractNote = {The UV-endonuclease approach to detect DNA damage has been combined with the alkaline elution technique with a resultant marked increase in sensitivity compared to the conventional method using alkaline sedimentation. DNA from UV-irradiated cells was digested on an inert filter with an extract from Micrococcus luteus and then analyzed by alkaline elution. Endonuclease-sensitive sites (endo-sites) were measured after doses of 0.08-0.7 Jm/sup -2/ of UV-radiation. An estimate of endo-site production with UV radiation, 0.27 endo-sites/10/sup 8/ daltons of DNA/0.1 Jm/sup -2/, was similar to that usually seen at higher doses by others. With repair incubation, approx. 50% of the endo-sites were removed in 4 h by normal human fibroblasts after 0.2 or 0.4 Jm/sup -2/, no appreciable repair was seen in xeroderma pigmentosum fibroblasts from complementation group A after 24 h of repair incubation. No photoreaction of UV damage due to 0.4 Jm/sup -2/ was detected in normal human fibroblasts.}
journal = {Mutat. Res.; (Netherlands)}
volume = {94}
journal type = {AC}
place = {Netherlands}
year = {1982}
month = {Jan}
}