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Purification and characterization of the d-xylose isomerase gene from Escherichia coli

Abstract

A DNA fragment containing both the Escherichia coli D-xylose isomerase (D-xylose ketol-isomerase, EC 5.3.1.5) gene and the D-xylulokinase (ATP: D-xylulose 5-phosphotransferase, EC 2.7.1.17) gene has been cloned on an E. coli plasmid. The D-xylose isomerase gene was separated from the D-xylulokinase gene by the construction of a new deletion plasmid, pLX7. The D-xylose isomerase gene cloned on pLX7 was found still to be an intact gene. The precise location of the D-xylose isomerase gene on the plasmid pLX7 was further determined by the construction of two more plasmids, pLX8 and pLX9. This is believed to be the first D-xylose isomerase gene that has been isolated and extensively purified from any organism. D-Xylose isomerase, the enzyme product of the D-xylose isomerase gene, is responsible for the conversion of D-xylose to D-xylulose, as well as D-glucose to D-fructose. It is widely believed that yeast cannot ferment D-xylose to ethanol primarily because of the lack of D-xylose isomerase in yeast. D-Xylose isomerase (also known as D-glucose isomerase) is also used for the commercial production of high-fructose syrups. The purification of the D-xylose isomerase gene may lead to the following industrial applications: (1) cloning and expression of the gene in yeast to make the  More>>
Publication Date:
Nov 01, 1983
Product Type:
Journal Article
Reference Number:
EDB-84-116159
Resource Relation:
Journal Name: Enzyme Microb. Technol.; (United Kingdom); Journal Volume: 5:6
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; ISOMERASES; PURIFICATION; YEASTS; GENETIC ENGINEERING; CLONING; ESCHERICHIA COLI; FRUCTOSE; GENETIC EFFECTS; PLASMIDS; RECOMBINANT DNA; XYLOSE; ALDEHYDES; BACTERIA; BIOLOGICAL EFFECTS; CARBOHYDRATES; CELL CONSTITUENTS; DNA; ENZYMES; FUNGI; HEXOSES; KETONES; MICROORGANISMS; MONOSACCHARIDES; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PENTOSES; PLANTS; SACCHARIDES; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550400 - Genetics
OSTI ID:
6762948
Research Organizations:
Purdue Univ., West Lafayette, IN
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: EMTED
Submitting Site:
HEDB
Size:
Pages: 417-420
Announcement Date:

Citation Formats

Ho, N W.Y., Rosenfeld, S, Stevis, P, and Tsao, G T. Purification and characterization of the d-xylose isomerase gene from Escherichia coli. United Kingdom: N. p., 1983. Web. doi:10.1016/0141-0229(83)90022-4.
Ho, N W.Y., Rosenfeld, S, Stevis, P, & Tsao, G T. Purification and characterization of the d-xylose isomerase gene from Escherichia coli. United Kingdom. doi:10.1016/0141-0229(83)90022-4.
Ho, N W.Y., Rosenfeld, S, Stevis, P, and Tsao, G T. 1983. "Purification and characterization of the d-xylose isomerase gene from Escherichia coli." United Kingdom. doi:10.1016/0141-0229(83)90022-4. https://www.osti.gov/servlets/purl/10.1016/0141-0229(83)90022-4.
@misc{etde_6762948,
title = {Purification and characterization of the d-xylose isomerase gene from Escherichia coli}
author = {Ho, N W.Y., Rosenfeld, S, Stevis, P, and Tsao, G T}
abstractNote = {A DNA fragment containing both the Escherichia coli D-xylose isomerase (D-xylose ketol-isomerase, EC 5.3.1.5) gene and the D-xylulokinase (ATP: D-xylulose 5-phosphotransferase, EC 2.7.1.17) gene has been cloned on an E. coli plasmid. The D-xylose isomerase gene was separated from the D-xylulokinase gene by the construction of a new deletion plasmid, pLX7. The D-xylose isomerase gene cloned on pLX7 was found still to be an intact gene. The precise location of the D-xylose isomerase gene on the plasmid pLX7 was further determined by the construction of two more plasmids, pLX8 and pLX9. This is believed to be the first D-xylose isomerase gene that has been isolated and extensively purified from any organism. D-Xylose isomerase, the enzyme product of the D-xylose isomerase gene, is responsible for the conversion of D-xylose to D-xylulose, as well as D-glucose to D-fructose. It is widely believed that yeast cannot ferment D-xylose to ethanol primarily because of the lack of D-xylose isomerase in yeast. D-Xylose isomerase (also known as D-glucose isomerase) is also used for the commercial production of high-fructose syrups. The purification of the D-xylose isomerase gene may lead to the following industrial applications: (1) cloning and expression of the gene in yeast to make the latter organism capable of directly fermenting D-xylose to ethanol, and (2) cloning of the gene on a high-copy-number plasmid in a proper host to overproduce the enzyme, which should have a profound impact on the high-fructose syrup technology. 14 references.}
doi = {10.1016/0141-0229(83)90022-4}
journal = {Enzyme Microb. Technol.; (United Kingdom)}
volume = {5:6}
journal type = {AC}
place = {United Kingdom}
year = {1983}
month = {Nov}
}