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W-reactivation of phage lambda in X-irradiated mutants of Escherichia coli K-12

Journal Article:

Abstract

The survival of UV irradiated phage lambda was increased on X-irradiated E.coli K-12 host cells over that on unirradiated cells. The frequency of c mutants among the surviving phages was increased to a similar extent by the X-ray exposure of the host cells as by UV light. This W-reactivation of phage lambda occurred in uvrA, polA, and recB mutants besides the wild type at about equal X-ray doses, but at a reduced reactivation efficiency compared with the wild type. W-reactivation was undetectable in recA mutants. While maximal UV induced W-reactivation occured 30 min after irradiation, the maximal X-ray induced reactivation was found immediately after irradiation. Chloramphenicol (100 ..mu..g/ml) and nitrofurantoin (50 ..mu..g/ml) inhibited W-reactivation of phage lambda if added before irradiation of the host cells, indicating the necessity of protein synthesis for W-reactivation.
Authors:
Martignoni, K D; Haselbacher, I [1] 
  1. Muenchen Univ. (Germany, F.R.). Strahlenbiologisches Inst.
Publication Date:
Jul 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-11-569929; EDB-81-018199
Resource Relation:
Journal Name: Radiat. Environ. Biophys.; (Germany, Federal Republic of); Journal Volume: 18:1
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BACTERIOPHAGES; BIOLOGICAL REPAIR; MUTANTS; RADIATION INJURIES; CHLORAMPHENICOL; ESCHERICHIA COLI; INHIBITION; IRRADIATION; SURVIVAL CURVES; ULTRAVIOLET RADIATION; X RADIATION; ANTIBIOTICS; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; DRUGS; ELECTROMAGNETIC RADIATION; INJURIES; IONIZING RADIATIONS; MICROORGANISMS; PARASITES; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; VIRUSES; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
6760045
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: REBPA
Submitting Site:
INIS
Size:
Pages: 27-36
Announcement Date:

Journal Article:

Citation Formats

Martignoni, K D, and Haselbacher, I. W-reactivation of phage lambda in X-irradiated mutants of Escherichia coli K-12. Germany: N. p., 1980. Web.
Martignoni, K D, & Haselbacher, I. W-reactivation of phage lambda in X-irradiated mutants of Escherichia coli K-12. Germany.
Martignoni, K D, and Haselbacher, I. 1980. "W-reactivation of phage lambda in X-irradiated mutants of Escherichia coli K-12." Germany.
@misc{etde_6760045,
title = {W-reactivation of phage lambda in X-irradiated mutants of Escherichia coli K-12}
author = {Martignoni, K D, and Haselbacher, I}
abstractNote = {The survival of UV irradiated phage lambda was increased on X-irradiated E.coli K-12 host cells over that on unirradiated cells. The frequency of c mutants among the surviving phages was increased to a similar extent by the X-ray exposure of the host cells as by UV light. This W-reactivation of phage lambda occurred in uvrA, polA, and recB mutants besides the wild type at about equal X-ray doses, but at a reduced reactivation efficiency compared with the wild type. W-reactivation was undetectable in recA mutants. While maximal UV induced W-reactivation occured 30 min after irradiation, the maximal X-ray induced reactivation was found immediately after irradiation. Chloramphenicol (100 ..mu..g/ml) and nitrofurantoin (50 ..mu..g/ml) inhibited W-reactivation of phage lambda if added before irradiation of the host cells, indicating the necessity of protein synthesis for W-reactivation.}
journal = {Radiat. Environ. Biophys.; (Germany, Federal Republic of)}
volume = {18:1}
journal type = {AC}
place = {Germany}
year = {1980}
month = {Jul}
}