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Two-site sandwich immunoradiometric assay of human lymphotoxin with monoclonal antibodies and its applications

Journal Article:

Abstract

Three monoclonal antibodies (MoAbs L49-15, L81-11 and L238-14) were raised against recombinant human lymphotoxin (rLT) derived from E. coli containing the cDNA sequence specifying LT. These MoAbs were ideal reagents for immunoassay of LT and a very sensitive, highly specific immunoradiometric assay (IRMA) was developed. This assay was rapid to perform and was capable of detecting as little as 10 pg/ml of LT. Application of the LT IRMA in combination with previously developed human gamma-interferon (IFN-..gamma..) and human tumour necrosis factor (TNF)-specific IRMA permitted independent estimations of these three substances to be carried out in parallel. By these means, it was found that RPMI 1788 lymphoblastoid cell line produced both LT and TNF, but not IFN-..gamma... Extensive analyses on cytokine (monokine and lymphokine) preparations derived from a variety of activated lymphocytes are also reported. Co-production of LT, TNF, and IFN-..gamma.. was a common finding, even occurring in alloantigen-specific T helper cell clones. 45 refs.; 3 figs.; 4 tabs.
Publication Date:
Nov 23, 1987
Product Type:
Journal Article
Reference Number:
AIX-19-100365; EDB-88-187103
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 104:1-2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; RADIOIMMUNOASSAY; LYMPHOCYTES; MONOCLONAL ANTIBODIES; MAN; ANIMAL CELLS; ANIMALS; ANTIBODIES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; LEUKOCYTES; MAMMALS; MATERIALS; PRIMATES; RADIOASSAY; RADIOIMMUNOLOGY; SOMATIC CELLS; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6757097
Research Organizations:
National Inst. for Biological Standards and Control, London (UK); Royal Postgraduate Medical School, London (UK); Christie Hospital and Holt Radium Inst., Manchester (UK). Paterson Labs.
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
INIS
Size:
Pages: 31-42
Announcement Date:

Journal Article:

Citation Formats

Meager, A, Parti, S, Leung, H, Woolley, J, Peil, E, Sidhu, S, and Roberts, T. Two-site sandwich immunoradiometric assay of human lymphotoxin with monoclonal antibodies and its applications. Netherlands: N. p., 1987. Web.
Meager, A, Parti, S, Leung, H, Woolley, J, Peil, E, Sidhu, S, & Roberts, T. Two-site sandwich immunoradiometric assay of human lymphotoxin with monoclonal antibodies and its applications. Netherlands.
Meager, A, Parti, S, Leung, H, Woolley, J, Peil, E, Sidhu, S, and Roberts, T. 1987. "Two-site sandwich immunoradiometric assay of human lymphotoxin with monoclonal antibodies and its applications." Netherlands.
@misc{etde_6757097,
title = {Two-site sandwich immunoradiometric assay of human lymphotoxin with monoclonal antibodies and its applications}
author = {Meager, A, Parti, S, Leung, H, Woolley, J, Peil, E, Sidhu, S, and Roberts, T}
abstractNote = {Three monoclonal antibodies (MoAbs L49-15, L81-11 and L238-14) were raised against recombinant human lymphotoxin (rLT) derived from E. coli containing the cDNA sequence specifying LT. These MoAbs were ideal reagents for immunoassay of LT and a very sensitive, highly specific immunoradiometric assay (IRMA) was developed. This assay was rapid to perform and was capable of detecting as little as 10 pg/ml of LT. Application of the LT IRMA in combination with previously developed human gamma-interferon (IFN-..gamma..) and human tumour necrosis factor (TNF)-specific IRMA permitted independent estimations of these three substances to be carried out in parallel. By these means, it was found that RPMI 1788 lymphoblastoid cell line produced both LT and TNF, but not IFN-..gamma... Extensive analyses on cytokine (monokine and lymphokine) preparations derived from a variety of activated lymphocytes are also reported. Co-production of LT, TNF, and IFN-..gamma.. was a common finding, even occurring in alloantigen-specific T helper cell clones. 45 refs.; 3 figs.; 4 tabs.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {104:1-2}
journal type = {AC}
place = {Netherlands}
year = {1987}
month = {Nov}
}