You need JavaScript to view this

Autoradiographic study of the penetration of radiolabelled dextrans and inulin through non-keratinized oral mucosa in vitro

Abstract

Although a well known barrier effect against the penetration of macromolecules exists at the basement membrane region of epithelial tissues, recent reports suggest that the penetration of smaller molecules may be impeded by this region. Considering the probable importance of the permeability of gingival crevicular tissues in the etiology of inflammatory periodontal disease, the present study was designed to evaluate the barrier function of the basement membrane region of non-keratinized oral mucosal epithelium to a series of radiolabelled penetrating molecules of increasing molecular weight. Tritium labeled inulin (Mw 5,000), dextran 20 (Mw 20,000) and dextran 70 (Mw 70,000) were used as penetrating molecules, and autoradiographic tracer techniques were used to evaluate the barrier function. The study was conducted in vitro to eliminate vascular ''wash-out'' effects and to facilitate study of penetration across the basement membrane region in both directions. The results indicated that although the penetration of inulin and dextran 70 was impeded by the basement membrane region, the penetration of dextran 20 was not affected. Therefore, the barrier function of the basement membrane region is not solely dependent on the molecular weight of the penetration molecule. Mechanisms to account for the findings are described and the significance to periodontal  More>>
Authors:
Alfano, M C; Chasens, A I; Masi, C W [1] 
  1. Block Periodontal Research Laboratories, Department of Periodontics and Oral Medicine, Fairleigh Dickinson University, School of Dentistry, Hackensack, New Jersey, U.S.A.
Publication Date:
Jan 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-09-373581; EDB-78-104850
Resource Relation:
Journal Name: J. Periodontal Res.; (Denmark); Journal Volume: 12
Subject:
59 BASIC BIOLOGICAL SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; DEXTRAN; MEMBRANE TRANSPORT; INULIN; MUCOUS MEMBRANES; PERMEABILITY; ORAL CAVITY; AUTORADIOGRAPHY; IN VITRO; TRITIUM COMPOUNDS; BLOOD SUBSTITUTES; CARBOHYDRATES; DIGESTIVE SYSTEM; LABELLED COMPOUNDS; ORGANIC COMPOUNDS; POLYSACCHARIDES; SACCHARIDES; 551001* - Physiological Systems- Tracer Techniques; 550601 - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6755555
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JPDRA
Submitting Site:
INIS
Size:
Pages: 368-377
Announcement Date:

Citation Formats

Alfano, M C, Chasens, A I, and Masi, C W. Autoradiographic study of the penetration of radiolabelled dextrans and inulin through non-keratinized oral mucosa in vitro. Denmark: N. p., 1977. Web. doi:10.1111/j.1600-0765.1977.tb01527.x.
Alfano, M C, Chasens, A I, & Masi, C W. Autoradiographic study of the penetration of radiolabelled dextrans and inulin through non-keratinized oral mucosa in vitro. Denmark. doi:10.1111/j.1600-0765.1977.tb01527.x.
Alfano, M C, Chasens, A I, and Masi, C W. 1977. "Autoradiographic study of the penetration of radiolabelled dextrans and inulin through non-keratinized oral mucosa in vitro." Denmark. doi:10.1111/j.1600-0765.1977.tb01527.x. https://www.osti.gov/servlets/purl/10.1111/j.1600-0765.1977.tb01527.x.
@misc{etde_6755555,
title = {Autoradiographic study of the penetration of radiolabelled dextrans and inulin through non-keratinized oral mucosa in vitro}
author = {Alfano, M C, Chasens, A I, and Masi, C W}
abstractNote = {Although a well known barrier effect against the penetration of macromolecules exists at the basement membrane region of epithelial tissues, recent reports suggest that the penetration of smaller molecules may be impeded by this region. Considering the probable importance of the permeability of gingival crevicular tissues in the etiology of inflammatory periodontal disease, the present study was designed to evaluate the barrier function of the basement membrane region of non-keratinized oral mucosal epithelium to a series of radiolabelled penetrating molecules of increasing molecular weight. Tritium labeled inulin (Mw 5,000), dextran 20 (Mw 20,000) and dextran 70 (Mw 70,000) were used as penetrating molecules, and autoradiographic tracer techniques were used to evaluate the barrier function. The study was conducted in vitro to eliminate vascular ''wash-out'' effects and to facilitate study of penetration across the basement membrane region in both directions. The results indicated that although the penetration of inulin and dextran 70 was impeded by the basement membrane region, the penetration of dextran 20 was not affected. Therefore, the barrier function of the basement membrane region is not solely dependent on the molecular weight of the penetration molecule. Mechanisms to account for the findings are described and the significance to periodontal disease is discussed.}
doi = {10.1111/j.1600-0765.1977.tb01527.x}
journal = {J. Periodontal Res.; (Denmark)}
volume = {12}
journal type = {AC}
place = {Denmark}
year = {1977}
month = {Jan}
}