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Enhancement of the effect of X-irradiation against cultured human glioblastoma cells by pretreatment with ACNU

Journal Article:

Abstract

Human glioblastoma A-7 (GB A-7) cells can apparently recover from potentially lethal X-irradiation. The authors, using a colony-forming assay, studied the influence of pretreatment with 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3- (2-chloroethyl)-3-nitrosourea hydrochloride (ACNU) on the effectiveness of X-irradiation against GB A-7 cells grown in monolayers and as multicellular spheroids. Pre-exposure to ACNU inhibited the recovery of irradiated GB A-7 cells. In monolayer cells, the combination treatment was most effective when ACNU was applied 2 to 8 hours prior to irradiation, and the larger the X-ray dose, the more potent the effect. ACNU pretreatment was more effective against large spheroids (enhancement ratio 1.86) than against small ones (1.34). Large spheroids showed necrosis, whereas small ones did not. Isobolographic analysis disclosed that the effect of combining X-irradiation and ACNU is within an additive envelope at the surviving fraction of 10{sup -2}, while supra-additive at the surviving fraction of 10{sup -3}. These results suggest that the potency of X-irradiation is augmented by ACNU pretreatment through an interactive mechanism. Further, suppression of recovery from X-ray induced potentially lethal damage was influenced by the presence of necrosis. (author).
Authors:
Mashiyama, Shoji; Katakura, Ryuichi; Takahashi, Kou; Kitahara, Masakazu; Suzuki, Jiro; [1]  Sasaki, Takehito
  1. Tohoku Univ., Sendai (Japan). Inst. of Brain Diseases
Publication Date:
Dec 01, 1989
Product Type:
Journal Article
Reference Number:
JPN-90-006187; EDB-90-119104
Resource Relation:
Journal Name: Neurologia Medico-Chirurgica; (Japan); Journal Volume: 29:12
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; TUMOR CELLS; BIOLOGICAL RADIATION EFFECTS; CELL CULTURES; CELL KILLING; GLIOMAS; NECROSIS; NITROSOUREAS; SPHEROIDS; SURVIVAL CURVES; X RADIATION; ANIMAL CELLS; BIOLOGICAL EFFECTS; DISEASES; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; NEOPLASMS; NITROSO COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PATHOLOGICAL CHANGES; RADIATION EFFECTS; RADIATIONS; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture
OSTI ID:
6748990
Country of Origin:
Japan
Language:
Japanese; English
Other Identifying Numbers:
Journal ID: ISSN 0387-2572; CODEN: NMCHB
Submitting Site:
JPN
Size:
Pages: 1070-1077
Announcement Date:
Aug 15, 1990

Journal Article:

Citation Formats

Mashiyama, Shoji, Katakura, Ryuichi, Takahashi, Kou, Kitahara, Masakazu, Suzuki, Jiro, and Sasaki, Takehito. Enhancement of the effect of X-irradiation against cultured human glioblastoma cells by pretreatment with ACNU. Japan: N. p., 1989. Web.
Mashiyama, Shoji, Katakura, Ryuichi, Takahashi, Kou, Kitahara, Masakazu, Suzuki, Jiro, & Sasaki, Takehito. Enhancement of the effect of X-irradiation against cultured human glioblastoma cells by pretreatment with ACNU. Japan.
Mashiyama, Shoji, Katakura, Ryuichi, Takahashi, Kou, Kitahara, Masakazu, Suzuki, Jiro, and Sasaki, Takehito. 1989. "Enhancement of the effect of X-irradiation against cultured human glioblastoma cells by pretreatment with ACNU." Japan.
@misc{etde_6748990,
title = {Enhancement of the effect of X-irradiation against cultured human glioblastoma cells by pretreatment with ACNU}
author = {Mashiyama, Shoji, Katakura, Ryuichi, Takahashi, Kou, Kitahara, Masakazu, Suzuki, Jiro, and Sasaki, Takehito}
abstractNote = {Human glioblastoma A-7 (GB A-7) cells can apparently recover from potentially lethal X-irradiation. The authors, using a colony-forming assay, studied the influence of pretreatment with 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3- (2-chloroethyl)-3-nitrosourea hydrochloride (ACNU) on the effectiveness of X-irradiation against GB A-7 cells grown in monolayers and as multicellular spheroids. Pre-exposure to ACNU inhibited the recovery of irradiated GB A-7 cells. In monolayer cells, the combination treatment was most effective when ACNU was applied 2 to 8 hours prior to irradiation, and the larger the X-ray dose, the more potent the effect. ACNU pretreatment was more effective against large spheroids (enhancement ratio 1.86) than against small ones (1.34). Large spheroids showed necrosis, whereas small ones did not. Isobolographic analysis disclosed that the effect of combining X-irradiation and ACNU is within an additive envelope at the surviving fraction of 10{sup -2}, while supra-additive at the surviving fraction of 10{sup -3}. These results suggest that the potency of X-irradiation is augmented by ACNU pretreatment through an interactive mechanism. Further, suppression of recovery from X-ray induced potentially lethal damage was influenced by the presence of necrosis. (author).}
journal = {Neurologia Medico-Chirurgica; (Japan)}
volume = {29:12}
journal type = {AC}
place = {Japan}
year = {1989}
month = {Dec}
}