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Gamma-radiolysis of some glycoproteins in dilute aqueous solutions

Abstract

A study has been made of the radiation-induced damage of some glycoproteins in dilute aqueous solutions. By use of specific radical scavengers, the roles of the individual free radicals, formed by ..gamma..-radiolysis, in causing damage has been assessed. The most effective radical in causing damage to human and porcine glycopolypeptide is the OH radical. The structure of the different blood group glycopolypeptides determines the sensitivity towards the free radical attack. The glycopolypeptide shows depolymerization and a characteristic absorption at approximately 270 nm due to the formation of additional products on irradiation. Chemical changes of the irradiated glycopolypeptide solutions revealed significant damage to the oligosaccharide chain and the polypeptide core of the glycopolypeptide. The radiation-induced inactivation of another glycoprotein, external yeast invertase, due to different radical species at pH 7.0 decreases in the following order: ea-barq > OH radical > (SCN) radical/sub 2//sup -/ > Br radical/sub 2//sup -/. The structure of this enzyme, accounts for the mechanism of enzyme inactivation and the relative damage of carbohydrate and amino acid residues. The irradiated enzyme solutions show significant changes in their electrophoretic behaviour on cellogel electrophoresis due to the formation of radiolysis products, which also show characteristic absorption maxima at approximately 275  More>>
Authors:
Publication Date:
Jan 01, 1981
Product Type:
Book
Reference Number:
AIX-15-001598; EDB-84-121273
Resource Relation:
Other Information: Thesis (Ph.D.)
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ENZYMES; RADIOLYSIS; GLUCOPROTEINS; BLOOD GROUPS; CATALYSIS; ELECTROPHORESIS; GAMMA RADIATION; HYDROXYL RADICALS; MAN; METABOLISM; MOLECULAR STRUCTURE; SWINE; YEASTS; ANIMALS; CARBOHYDRATES; CHEMICAL RADIATION EFFECTS; CHEMICAL REACTIONS; CHEMISTRY; DECOMPOSITION; DOMESTIC ANIMALS; ELECTROMAGNETIC RADIATION; FUNGI; IONIZING RADIATIONS; MAMMALS; MICROORGANISMS; ORGANIC COMPOUNDS; PLANTS; PRIMATES; PROTEINS; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; RADICALS; SACCHARIDES; VERTEBRATES; 560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
OSTI ID:
6748673
Country of Origin:
United Kingdom
Language:
English
Availability:
British Library, Boston Spa, Wetherby, West Yorks. No. D39650/82.
Submitting Site:
INIS
Size:
Pages: 222
Announcement Date:

Citation Formats

Nagrani, S. Gamma-radiolysis of some glycoproteins in dilute aqueous solutions. United Kingdom: N. p., 1981. Web.
Nagrani, S. Gamma-radiolysis of some glycoproteins in dilute aqueous solutions. United Kingdom.
Nagrani, S. 1981. "Gamma-radiolysis of some glycoproteins in dilute aqueous solutions." United Kingdom.
@misc{etde_6748673,
title = {Gamma-radiolysis of some glycoproteins in dilute aqueous solutions}
author = {Nagrani, S}
abstractNote = {A study has been made of the radiation-induced damage of some glycoproteins in dilute aqueous solutions. By use of specific radical scavengers, the roles of the individual free radicals, formed by ..gamma..-radiolysis, in causing damage has been assessed. The most effective radical in causing damage to human and porcine glycopolypeptide is the OH radical. The structure of the different blood group glycopolypeptides determines the sensitivity towards the free radical attack. The glycopolypeptide shows depolymerization and a characteristic absorption at approximately 270 nm due to the formation of additional products on irradiation. Chemical changes of the irradiated glycopolypeptide solutions revealed significant damage to the oligosaccharide chain and the polypeptide core of the glycopolypeptide. The radiation-induced inactivation of another glycoprotein, external yeast invertase, due to different radical species at pH 7.0 decreases in the following order: ea-barq > OH radical > (SCN) radical/sub 2//sup -/ > Br radical/sub 2//sup -/. The structure of this enzyme, accounts for the mechanism of enzyme inactivation and the relative damage of carbohydrate and amino acid residues. The irradiated enzyme solutions show significant changes in their electrophoretic behaviour on cellogel electrophoresis due to the formation of radiolysis products, which also show characteristic absorption maxima at approximately 275 nm. (author).}
place = {United Kingdom}
year = {1981}
month = {Jan}
}