Abstract
MacConkey agar and Cystine-Lactose-Electrolyte-Deficient (CLED) agar, media widely used in the bacteriological laboratory and recommended for the detection of urinary tract infections, were sterilized by gamma-radiation at a dose of 1.5 Mrad. Both were modified and adapted to radiation sterilization by adding sodium thioglycollate as a radioprotectant, and by increasing their indicator content. The media performed well when tested with different Enterobacteria and other micro-organisms. Growth and change of indicator reaction were equal in irradiated and autoclaved culture media. Culture media were also evaluated after storage for one month at room temperature and at 4 degrees C and compared well with freshly autoclaved media.
Citation Formats
Bogokowsky, B, Eisenberg, E, and Altmann, G.
Sterilization of MacConkey agar and CLED medium by gamma-radiation.
United Kingdom: N. p.,
1983.
Web.
doi:10.1016/0020-708X(83)90044-3.
Bogokowsky, B, Eisenberg, E, & Altmann, G.
Sterilization of MacConkey agar and CLED medium by gamma-radiation.
United Kingdom.
https://doi.org/10.1016/0020-708X(83)90044-3
Bogokowsky, B, Eisenberg, E, and Altmann, G.
1983.
"Sterilization of MacConkey agar and CLED medium by gamma-radiation."
United Kingdom.
https://doi.org/10.1016/0020-708X(83)90044-3.
@misc{etde_6748638,
title = {Sterilization of MacConkey agar and CLED medium by gamma-radiation}
author = {Bogokowsky, B, Eisenberg, E, and Altmann, G}
abstractNote = {MacConkey agar and Cystine-Lactose-Electrolyte-Deficient (CLED) agar, media widely used in the bacteriological laboratory and recommended for the detection of urinary tract infections, were sterilized by gamma-radiation at a dose of 1.5 Mrad. Both were modified and adapted to radiation sterilization by adding sodium thioglycollate as a radioprotectant, and by increasing their indicator content. The media performed well when tested with different Enterobacteria and other micro-organisms. Growth and change of indicator reaction were equal in irradiated and autoclaved culture media. Culture media were also evaluated after storage for one month at room temperature and at 4 degrees C and compared well with freshly autoclaved media.}
doi = {10.1016/0020-708X(83)90044-3}
journal = []
volume = {34:10}
journal type = {AC}
place = {United Kingdom}
year = {1983}
month = {Oct}
}
title = {Sterilization of MacConkey agar and CLED medium by gamma-radiation}
author = {Bogokowsky, B, Eisenberg, E, and Altmann, G}
abstractNote = {MacConkey agar and Cystine-Lactose-Electrolyte-Deficient (CLED) agar, media widely used in the bacteriological laboratory and recommended for the detection of urinary tract infections, were sterilized by gamma-radiation at a dose of 1.5 Mrad. Both were modified and adapted to radiation sterilization by adding sodium thioglycollate as a radioprotectant, and by increasing their indicator content. The media performed well when tested with different Enterobacteria and other micro-organisms. Growth and change of indicator reaction were equal in irradiated and autoclaved culture media. Culture media were also evaluated after storage for one month at room temperature and at 4 degrees C and compared well with freshly autoclaved media.}
doi = {10.1016/0020-708X(83)90044-3}
journal = []
volume = {34:10}
journal type = {AC}
place = {United Kingdom}
year = {1983}
month = {Oct}
}