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Use of avidin-biotin-peroxidase complex for measurement of UV lesions in human DNA by microELISA

Journal Article:

Abstract

The avidin/biotin system was introduced into the standard enzyme-linked immunosorbent assay (ELISA) to increase its sensitivity for detecting UV lesions in human DNA. Goat anti-rabbit IgG-peroxidase used in the standard ELISA as second antibody was replaced by biotinylated goat anti-rabbit IgG plus the avidin-biotin-peroxidase complex (ABC) reagent. Sensitivity of detection of plate-fixed UV-DNA-antibody complexes was increased about 8-fold and photolesions in human DNA samples irradiated with as low a dose as 1 J/m/sup 2/ UVC or a suberythermal dose of UVB light could be detected.
Authors:
Leipold, B; [1]  Remy, W [2] 
  1. Technischen Universitaet Muenchen (Germany, F.R.). Dermatologische Klinik
  2. Max-Planck-Institut fuer Biochemie, Muenchen (Germany, F.R.)
Publication Date:
Feb 10, 1984
Product Type:
Journal Article
Reference Number:
AIX-15-035762; EDB-84-121271
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 66:2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BIOASSAY; SENSITIVITY; CHEMICAL RADIATION EFFECTS; DNA; BIOADSORBENTS; BIOTIN; CHEMICAL ANALYSIS; IMMUNOLOGY; NEAR ULTRAVIOLET RADIATION; PEROXIDASES; ADSORBENTS; AZOLES; CARBOXYLIC ACIDS; CHEMISTRY; ELECTROMAGNETIC RADIATION; ENZYMES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; IMIDAZOLES; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; OXIDOREDUCTASES; PORPHYRINS; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; ULTRAVIOLET RADIATION; VITAMIN B GROUP; VITAMINS; 560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
OSTI ID:
6743145
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
HEDB
Size:
Pages: 227-234
Announcement Date:
May 01, 1984

Journal Article:

Citation Formats

Leipold, B, and Remy, W. Use of avidin-biotin-peroxidase complex for measurement of UV lesions in human DNA by microELISA. Netherlands: N. p., 1984. Web.
Leipold, B, & Remy, W. Use of avidin-biotin-peroxidase complex for measurement of UV lesions in human DNA by microELISA. Netherlands.
Leipold, B, and Remy, W. 1984. "Use of avidin-biotin-peroxidase complex for measurement of UV lesions in human DNA by microELISA." Netherlands.
@misc{etde_6743145,
title = {Use of avidin-biotin-peroxidase complex for measurement of UV lesions in human DNA by microELISA}
author = {Leipold, B, and Remy, W}
abstractNote = {The avidin/biotin system was introduced into the standard enzyme-linked immunosorbent assay (ELISA) to increase its sensitivity for detecting UV lesions in human DNA. Goat anti-rabbit IgG-peroxidase used in the standard ELISA as second antibody was replaced by biotinylated goat anti-rabbit IgG plus the avidin-biotin-peroxidase complex (ABC) reagent. Sensitivity of detection of plate-fixed UV-DNA-antibody complexes was increased about 8-fold and photolesions in human DNA samples irradiated with as low a dose as 1 J/m/sup 2/ UVC or a suberythermal dose of UVB light could be detected.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {66:2}
journal type = {AC}
place = {Netherlands}
year = {1984}
month = {Feb}
}