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Production of alpha-amylase in batch and chemostat culture by bacillus stearothermophilus

Abstract

The production of alpha-amylase by a strain of B.stearothermophilus isolated from leaf litter was investigated in a tryptone-maltose medium at 55 degrees in batch and chemostat culture. Amylase production was growth-limited and restricted to the exponential phase in batch culture. The enzyme yield was reduced by 40% when the culture pH was maintained at pH 7.2. Amylase production in chemostat culture was influenced by the growth rate throughout the dilution rate range used.
Publication Date:
Jan 01, 1980
Product Type:
Journal Article
Reference Number:
EDB-83-003379
Resource Relation:
Journal Name: Antonie van Leeuwenhoek; (Netherlands); Journal Volume: 46:4
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; AMYLASE; BIOSYNTHESIS; BACILLUS; CULTURE MEDIA; BACTERIA; ENZYMES; GLYCOSYL HYDROLASES; HYDROLASES; MICROORGANISMS; O-GLYCOSYL HYDROLASES; SYNTHESIS; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550700 - Microbiology
OSTI ID:
6742041
Research Organizations:
Sch Nat Sci, Hatfield Polytech, Hatfield/Hertfordshire, Engl AL10 9AB
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ALJMA
Submitting Site:
HEDB
Size:
Pages: 391-398
Announcement Date:

Citation Formats

Davis, P E, Cohen, D L, and Whitaker, A. Production of alpha-amylase in batch and chemostat culture by bacillus stearothermophilus. Netherlands: N. p., 1980. Web. doi:10.1007/BF00421985.
Davis, P E, Cohen, D L, & Whitaker, A. Production of alpha-amylase in batch and chemostat culture by bacillus stearothermophilus. Netherlands. doi:10.1007/BF00421985.
Davis, P E, Cohen, D L, and Whitaker, A. 1980. "Production of alpha-amylase in batch and chemostat culture by bacillus stearothermophilus." Netherlands. doi:10.1007/BF00421985. https://www.osti.gov/servlets/purl/10.1007/BF00421985.
@misc{etde_6742041,
title = {Production of alpha-amylase in batch and chemostat culture by bacillus stearothermophilus}
author = {Davis, P E, Cohen, D L, and Whitaker, A}
abstractNote = {The production of alpha-amylase by a strain of B.stearothermophilus isolated from leaf litter was investigated in a tryptone-maltose medium at 55 degrees in batch and chemostat culture. Amylase production was growth-limited and restricted to the exponential phase in batch culture. The enzyme yield was reduced by 40% when the culture pH was maintained at pH 7.2. Amylase production in chemostat culture was influenced by the growth rate throughout the dilution rate range used.}
doi = {10.1007/BF00421985}
journal = {Antonie van Leeuwenhoek; (Netherlands)}
volume = {46:4}
journal type = {AC}
place = {Netherlands}
year = {1980}
month = {Jan}
}