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Radioligand assays - methods and applications. IV. Uniform regression of hyperbolic and linear radioimmunoassay calibration curves

Abstract

In order to handle all types of radioimmunoassay (RIA) calibration curves obtained in the authors' laboratory in the same way, they tried to find a non-linear expression for their regression which allows calibration curves with different degrees of curvature to be fitted. Considering the two boundary cases of the incubation protocol they derived a hyperbolic inverse regression function: x = a/sub 1/y + a/sub 0/ + asub(-1)y/sup -1/, where x is the total concentration of antigen, asub(i) are constants, and y is the specifically bound radioactivity. An RIA evaluation procedure based on this function is described providing a fitted inverse RIA calibration curve and some statistical quality parameters. The latter are of an order which is normal for RIA systems. There is an excellent agreement between fitted and experimentally obtained calibration curves having a different degree of curvature.
Authors:
Keilacker, H; Becker, G; Ziegler, M; Gottschling, H D [1] 
  1. Zentralinstitut fuer Diabetes, Karlsburg (German Democratic Republic)
Publication Date:
Oct 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-12-575444; EDB-81-017837
Resource Relation:
Journal Name: J. Biochem. Biophys. Methods; (Netherlands); Journal Volume: 3:4
Subject:
59 BASIC BIOLOGICAL SCIENCES; RADIOIMMUNOASSAY; CALIBRATION; BLOOD PLASMA; DIAGRAMS; GLUCAGON; INSULIN; MATHEMATICAL MODELS; STANDARDIZATION; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; HORMONES; ISOTOPE APPLICATIONS; MATERIALS; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PEPTIDES; POLYPEPTIDES; PROTEINS; RADIOASSAY; TRACER TECHNIQUES; 550201* - Biochemistry- Tracer Techniques
OSTI ID:
6741824
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JBBMD
Submitting Site:
INIS
Size:
Pages: 225-231
Announcement Date:

Citation Formats

Keilacker, H, Becker, G, Ziegler, M, and Gottschling, H D. Radioligand assays - methods and applications. IV. Uniform regression of hyperbolic and linear radioimmunoassay calibration curves. Netherlands: N. p., 1980. Web. doi:10.1016/0165-022X(80)90061-5.
Keilacker, H, Becker, G, Ziegler, M, & Gottschling, H D. Radioligand assays - methods and applications. IV. Uniform regression of hyperbolic and linear radioimmunoassay calibration curves. Netherlands. doi:10.1016/0165-022X(80)90061-5.
Keilacker, H, Becker, G, Ziegler, M, and Gottschling, H D. 1980. "Radioligand assays - methods and applications. IV. Uniform regression of hyperbolic and linear radioimmunoassay calibration curves." Netherlands. doi:10.1016/0165-022X(80)90061-5. https://www.osti.gov/servlets/purl/10.1016/0165-022X(80)90061-5.
@misc{etde_6741824,
title = {Radioligand assays - methods and applications. IV. Uniform regression of hyperbolic and linear radioimmunoassay calibration curves}
author = {Keilacker, H, Becker, G, Ziegler, M, and Gottschling, H D}
abstractNote = {In order to handle all types of radioimmunoassay (RIA) calibration curves obtained in the authors' laboratory in the same way, they tried to find a non-linear expression for their regression which allows calibration curves with different degrees of curvature to be fitted. Considering the two boundary cases of the incubation protocol they derived a hyperbolic inverse regression function: x = a/sub 1/y + a/sub 0/ + asub(-1)y/sup -1/, where x is the total concentration of antigen, asub(i) are constants, and y is the specifically bound radioactivity. An RIA evaluation procedure based on this function is described providing a fitted inverse RIA calibration curve and some statistical quality parameters. The latter are of an order which is normal for RIA systems. There is an excellent agreement between fitted and experimentally obtained calibration curves having a different degree of curvature.}
doi = {10.1016/0165-022X(80)90061-5}
journal = {J. Biochem. Biophys. Methods; (Netherlands)}
volume = {3:4}
journal type = {AC}
place = {Netherlands}
year = {1980}
month = {Oct}
}