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Resistance of 3T3 mouse cells to UV light in relation to excision and transfer of dimers to daughter strands

Abstract

Mouse cells (3T3 line) and human fibroblasts are equally sensitive to UV light. At fluences of 2.0-2.5 J/m/sup 2/ mouse cells excise only 20% of the pyrimidine dimers as compared to 80% excised by human fibroblasts. This fluence allows 37% survival in both cases. Hence, mouse cells are more resistant to the same burden of unexcised dimers. The reason for this increased tolerance to dimers does not seem to be due to a recombinational mechanism, as judged by the fact that only ca. 5% of the dimers are transferred from parental to daughter strands. The transfer of dimers was measured by the Micrococcus luteus UV endonuclease assay, irradiating cells at G/sub 1/ to avoid artifacts arising from introduction of dimers in nascent strands. The possibility of other mechanisms being involved in the process of tolerance to DNA lesions is discussed.
Authors:
Menck, C F.M.; Meneghini, R [1] 
  1. Sao Paulo Univ. (Brazil). Inst. de Quimica
Publication Date:
Apr 01, 1982
Product Type:
Journal Article
Reference Number:
AIX-13-705452; EDB-83-009064
Resource Relation:
Journal Name: Photochem. Photobiol.; (United Kingdom); Journal Volume: 35:4
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CELL CULTURES; PHOTOSENSITIVITY; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REPAIR; DIMERS; FIBROBLASTS; MAN; MICE; PYRIMIDINES; SURVIVAL CURVES; ULTRAVIOLET RADIATION; ANIMAL CELLS; ANIMALS; AZINES; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; MAMMALS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PRIMATES; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; RODENTS; SENSITIVITY; SOMATIC CELLS; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987)
OSTI ID:
6723310
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PHCBA
Submitting Site:
HEDB
Size:
Pages: 507-513
Announcement Date:

Citation Formats

Menck, C F.M., and Meneghini, R. Resistance of 3T3 mouse cells to UV light in relation to excision and transfer of dimers to daughter strands. United Kingdom: N. p., 1982. Web. doi:10.1111/j.1751-1097.1982.tb02601.x.
Menck, C F.M., & Meneghini, R. Resistance of 3T3 mouse cells to UV light in relation to excision and transfer of dimers to daughter strands. United Kingdom. doi:10.1111/j.1751-1097.1982.tb02601.x.
Menck, C F.M., and Meneghini, R. 1982. "Resistance of 3T3 mouse cells to UV light in relation to excision and transfer of dimers to daughter strands." United Kingdom. doi:10.1111/j.1751-1097.1982.tb02601.x. https://www.osti.gov/servlets/purl/10.1111/j.1751-1097.1982.tb02601.x.
@misc{etde_6723310,
title = {Resistance of 3T3 mouse cells to UV light in relation to excision and transfer of dimers to daughter strands}
author = {Menck, C F.M., and Meneghini, R}
abstractNote = {Mouse cells (3T3 line) and human fibroblasts are equally sensitive to UV light. At fluences of 2.0-2.5 J/m/sup 2/ mouse cells excise only 20% of the pyrimidine dimers as compared to 80% excised by human fibroblasts. This fluence allows 37% survival in both cases. Hence, mouse cells are more resistant to the same burden of unexcised dimers. The reason for this increased tolerance to dimers does not seem to be due to a recombinational mechanism, as judged by the fact that only ca. 5% of the dimers are transferred from parental to daughter strands. The transfer of dimers was measured by the Micrococcus luteus UV endonuclease assay, irradiating cells at G/sub 1/ to avoid artifacts arising from introduction of dimers in nascent strands. The possibility of other mechanisms being involved in the process of tolerance to DNA lesions is discussed.}
doi = {10.1111/j.1751-1097.1982.tb02601.x}
journal = {Photochem. Photobiol.; (United Kingdom)}
volume = {35:4}
journal type = {AC}
place = {United Kingdom}
year = {1982}
month = {Apr}
}