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DMSO-induced dehydration of DPPC membranes studied by x-ray diffraction, small angle neutron scattering and calorimetry

Abstract

The properties of dimethyl sulfoxide (DMSO), a cryoprotector well known for its biological and therapeutic applications, were investigated on lipid membranes by x-ray diffraction, differential scanning calorimetry (DSC) and small angle neutron scattering (SANS). The DSC study of water freezing and melting of ice was performed in the ternary system which consists of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC)/DMSO/water system. The influence of DMSO on the DPPC membrane structure was established in the excess of solvent in the region of DMSO mole fraction from 0.0 to 1.0. The methods applied demonstrated the differences in the membrane structure in three sub-regions of DMSO mole fraction (X{sub DMSO}) from 0.0 to 0.3 for the first, from 0.3 to 0.9 for the second, and from 0.9 to 1.0 for the third sub-region. The results for 0.0 {<=} X{sub DMSO} {<=} 0.3 can be explained in the framework of DMSO-induced dehydration of intermembrane space 11 refs., 7 figs. Submitted to the Conference `ISSRNS`98`, 15-20 Jun 1998, Ustron-Jaszowiec, Poland
Authors:
Kiselev, M A; Kiselev, A M; [1]  Lesieur, P; [2]  Grabielle-Madelmond, C; Ollivon, M [3] 
  1. Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna (Russian Federation)
  2. LURE, Universite Paris-Sud, Bat. 209-D, F91405 Orsay cedex, (France)
  3. Physico-Chimie des systemes polyphases, URA 1218 du CNRS, Faculte de Pharmacie, tour B, F-92296, Chatenay Malabry (France)
Publication Date:
Dec 01, 1998
Product Type:
Technical Report
Report Number:
JINR-E-14-98-91
Reference Number:
SCA: 665100; PA: AIX-29:062939; EDB-98:123681; SN: 98002035234
Resource Relation:
Other Information: DN: 11 refs., 7 figs. Submitted to the Conference `ISSRNS`98`, 15-20 Jun 1998, Ustron-Jaszowiec, Poland; PBD: 1998
Subject:
66 PHYSICS; CALORIMETRY; CRYSTALLIZATION; DEHYDRATION; DMSO; MELTING; NEUTRON DIFFRACTION; PHOSPHOLIPIDS; SAMPLE PREPARATION; SMALL ANGLE SCATTERING; TEMPERATURE DEPENDENCE; TEMPERATURE RANGE 0273-0400 K; X-RAY DIFFRACTION
OSTI ID:
671209
Research Organizations:
Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna (Russian Federation)
Country of Origin:
JINR
Language:
English
Other Identifying Numbers:
Other: ON: DE99607970; TRN: XJ9800286062939
Availability:
INIS; OSTI as DE99607970
Submitting Site:
INIS
Size:
10 p.
Announcement Date:

Citation Formats

Kiselev, M A, Kiselev, A M, Lesieur, P, Grabielle-Madelmond, C, and Ollivon, M. DMSO-induced dehydration of DPPC membranes studied by x-ray diffraction, small angle neutron scattering and calorimetry. JINR: N. p., 1998. Web.
Kiselev, M A, Kiselev, A M, Lesieur, P, Grabielle-Madelmond, C, & Ollivon, M. DMSO-induced dehydration of DPPC membranes studied by x-ray diffraction, small angle neutron scattering and calorimetry. JINR.
Kiselev, M A, Kiselev, A M, Lesieur, P, Grabielle-Madelmond, C, and Ollivon, M. 1998. "DMSO-induced dehydration of DPPC membranes studied by x-ray diffraction, small angle neutron scattering and calorimetry." JINR.
@misc{etde_671209,
title = {DMSO-induced dehydration of DPPC membranes studied by x-ray diffraction, small angle neutron scattering and calorimetry}
author = {Kiselev, M A, Kiselev, A M, Lesieur, P, Grabielle-Madelmond, C, and Ollivon, M}
abstractNote = {The properties of dimethyl sulfoxide (DMSO), a cryoprotector well known for its biological and therapeutic applications, were investigated on lipid membranes by x-ray diffraction, differential scanning calorimetry (DSC) and small angle neutron scattering (SANS). The DSC study of water freezing and melting of ice was performed in the ternary system which consists of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC)/DMSO/water system. The influence of DMSO on the DPPC membrane structure was established in the excess of solvent in the region of DMSO mole fraction from 0.0 to 1.0. The methods applied demonstrated the differences in the membrane structure in three sub-regions of DMSO mole fraction (X{sub DMSO}) from 0.0 to 0.3 for the first, from 0.3 to 0.9 for the second, and from 0.9 to 1.0 for the third sub-region. The results for 0.0 {<=} X{sub DMSO} {<=} 0.3 can be explained in the framework of DMSO-induced dehydration of intermembrane space 11 refs., 7 figs. Submitted to the Conference `ISSRNS`98`, 15-20 Jun 1998, Ustron-Jaszowiec, Poland}
place = {JINR}
year = {1998}
month = {Dec}
}