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Evaluation of technetium-99m exametazime stabilised with cobalt chloride as a blood flow tracer in focal cerebral ischaemia

Abstract

A protocol has been devised to effectively extend the limited post-reconstitution shelf life of technetium-99m exametazime as a radiopharmaceutical for imaging cerebral blood flow (CBF) distribution. The potential of [sup 99m]Tc-exametazime stabilised with cobalt chloride for imaging CBF distribution as late as 4 h after reconstitution has been examined in ischaemic and non-ischaemic tissue in halothane-anaesthetised cats. Focal cerebral ischaemia was produced by permanent middle cerebral artery occlusion. The relationship between [sup 99m]Tc-exametazime uptake and retention and CBF (assessed with [[sup 14]C]iodoantipyrine 10 min after first radiopharmaceutical administration) was determined in the same tissue section with double label autoradiography. Over the CBF range 0 - 80 ml 100 g[sup -1] min[sup -1], the uptake of [sup 99m]Tc-exametazime (quantitatively and topographically) was linearly related to CBF irrespective of whether the [sup 99m]Tc-labelled tracer was unstabilised (and administered within 10 min of reconstitution) or was stabilised with cobalt chloride (and administered up to 240 min after reconstitution). For levels of CBF in excess of 80 ml 100 g[sup -1] min[sup -1] the excellent topographical relationship between [sup 99m]Tc-exametazime distribution and CBF is maintained but quantitatively, [sup 99m]Tc-exametazime underestimates CBF to a similar degree in animals receiving stabilised and unstabilised [sup 99m]Tc-exametazime. The  More>>
Authors:
Gartshore, G; [1]  Bannan, P; [1]  Patterson, J; [1]  Higley, B; [1]  McCulloch, J [1] 
  1. Wellcome Surgical Inst. and Hugh Fraser Neuroscience Labs., Glasgow Univ. (United Kingdom)
Publication Date:
Sep 01, 1994
Product Type:
Journal Article
Reference Number:
DEN-94-0FP039; EDB-95-024972
Resource Relation:
Journal Name: European Journal of Nuclear Medicine; (Germany); Journal Volume: 21:9
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; BRAIN; BLOOD FLOW; ISCHEMIA; SINGLE PHOTON EMISSION COMPUTED TOMOGRAPHY; AUTORADIOGRAPHY; BLOOD CIRCULATION; CARBON 14; CATS; COBALT CHLORIDES; RADIOPHARMACEUTICALS; TECHNETIUM 99; TECHNETIUM COMPLEXES; ANEMIAS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARBON ISOTOPES; CARDIOVASCULAR DISEASES; CENTRAL NERVOUS SYSTEM; CHLORIDES; CHLORINE COMPOUNDS; COBALT COMPOUNDS; COMPLEXES; COMPUTERIZED TOMOGRAPHY; DIAGNOSTIC TECHNIQUES; DISEASES; DRUGS; EMISSION COMPUTED TOMOGRAPHY; EVEN-EVEN NUCLEI; HALIDES; HALOGEN COMPOUNDS; HEMIC DISEASES; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LABELLED COMPOUNDS; LIGHT NUCLEI; MAMMALS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANS; RADIOISOTOPES; SYMPTOMS; TECHNETIUM ISOTOPES; TOMOGRAPHY; TRANSITION ELEMENT COMPLEXES; TRANSITION ELEMENT COMPOUNDS; VASCULAR DISEASES; VERTEBRATES; YEARS LIVING RADIOI; YEARS LIVING RADIOISOTOPES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6699946
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0340-6997; CODEN: EJNMD9
Submitting Site:
DEN
Size:
Pages: 913-923
Announcement Date:
Feb 15, 1995

Citation Formats

Gartshore, G, Bannan, P, Patterson, J, Higley, B, and McCulloch, J. Evaluation of technetium-99m exametazime stabilised with cobalt chloride as a blood flow tracer in focal cerebral ischaemia. Germany: N. p., 1994. Web.
Gartshore, G, Bannan, P, Patterson, J, Higley, B, & McCulloch, J. Evaluation of technetium-99m exametazime stabilised with cobalt chloride as a blood flow tracer in focal cerebral ischaemia. Germany.
Gartshore, G, Bannan, P, Patterson, J, Higley, B, and McCulloch, J. 1994. "Evaluation of technetium-99m exametazime stabilised with cobalt chloride as a blood flow tracer in focal cerebral ischaemia." Germany.
@misc{etde_6699946,
title = {Evaluation of technetium-99m exametazime stabilised with cobalt chloride as a blood flow tracer in focal cerebral ischaemia}
author = {Gartshore, G, Bannan, P, Patterson, J, Higley, B, and McCulloch, J}
abstractNote = {A protocol has been devised to effectively extend the limited post-reconstitution shelf life of technetium-99m exametazime as a radiopharmaceutical for imaging cerebral blood flow (CBF) distribution. The potential of [sup 99m]Tc-exametazime stabilised with cobalt chloride for imaging CBF distribution as late as 4 h after reconstitution has been examined in ischaemic and non-ischaemic tissue in halothane-anaesthetised cats. Focal cerebral ischaemia was produced by permanent middle cerebral artery occlusion. The relationship between [sup 99m]Tc-exametazime uptake and retention and CBF (assessed with [[sup 14]C]iodoantipyrine 10 min after first radiopharmaceutical administration) was determined in the same tissue section with double label autoradiography. Over the CBF range 0 - 80 ml 100 g[sup -1] min[sup -1], the uptake of [sup 99m]Tc-exametazime (quantitatively and topographically) was linearly related to CBF irrespective of whether the [sup 99m]Tc-labelled tracer was unstabilised (and administered within 10 min of reconstitution) or was stabilised with cobalt chloride (and administered up to 240 min after reconstitution). For levels of CBF in excess of 80 ml 100 g[sup -1] min[sup -1] the excellent topographical relationship between [sup 99m]Tc-exametazime distribution and CBF is maintained but quantitatively, [sup 99m]Tc-exametazime underestimates CBF to a similar degree in animals receiving stabilised and unstabilised [sup 99m]Tc-exametazime. The presence of the stabiliser, cobalt chloride, extends greatly the period over which [sup 99m]Tc-exametazime can be used after reconstitution to generate images of CBF distribution in normal and ischaemic cerebral tissue. (orig.)}
journal = []
volume = {21:9}
journal type = {AC}
place = {Germany}
year = {1994}
month = {Sep}
}