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Nuclear magnetic resonance (NMR) spectroscopy and its application to biomedical research

Journal Article:

Abstract

The principles of nuclear magnetic resonance (NMR) spectroscopy were explained and its application to biomedical research discussed. With /sup 31/P-NMR, it is feasible to conduct a continuous, non-invasive measurement of the contents of myocardial high-energy phosphate compounds and the intracellular pH (determined by monitoring the pH dependent shift of the inorganic phosphate peak relative to that of creatine phosphate), and to correlate them with the mechanical function. The determination of the free magnesium concentration is also possible on a similar principle to that for pH determination (the shift of MgATP peaks relative to ATP is utilized in this case). It is estimated to be 0.3 mM and was found not to be changed during ischemia. Several examples of studies including our own conducted to delineate the ischemic derangements of the myocardial energy metabolism and the effects of various interventions thereupon were illustrated. Finally a brief mention was made of the saturation transfer technique. This is the only method with which one can study the kinetics of the enzyme reactions under in vivo conditions. The application of the method for analysis of the creatine kinase reaction and the ATP synthesis was demonstrated. (author) 49 refs.
Publication Date:
Jul 01, 1988
Product Type:
Journal Article
Reference Number:
JPN-88-084110; EDB-88-194865
Resource Relation:
Journal Name: Nippon Yakurigaku Zasshi; (Japan); Journal Volume: 92:1
Subject:
60 APPLIED LIFE SCIENCES; METABOLISM; NMR SPECTRA; MYOCARDIUM; ATP; CHEMICAL SHIFT; CREATINE; ISCHEMIA; NUCLEAR MAGNETIC RESONANCE; PHOSPHORUS 31; REVIEWS; AMINO ACIDS; BODY; CARBOXYLIC ACIDS; CARDIOVASCULAR DISEASES; CARDIOVASCULAR SYSTEM; DISEASES; DOCUMENT TYPES; HEART; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MUSCLES; NUCLEI; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; RESONANCE; SPECTRA; STABLE ISOTOPES; VASCULAR DISEASES; 553006* - Agriculture & Food Technology- Other Agricultural Applications- (1987-)
OSTI ID:
6699840
Research Organizations:
Niigata Univ. (Japan). School of Medicine
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: NYKZA
Submitting Site:
JPN
Size:
Pages: 1-9
Announcement Date:
Dec 01, 1988

Journal Article:

Citation Formats

Nakazawa, Mikio, and Imai, Shoichi. Nuclear magnetic resonance (NMR) spectroscopy and its application to biomedical research. Japan: N. p., 1988. Web.
Nakazawa, Mikio, & Imai, Shoichi. Nuclear magnetic resonance (NMR) spectroscopy and its application to biomedical research. Japan.
Nakazawa, Mikio, and Imai, Shoichi. 1988. "Nuclear magnetic resonance (NMR) spectroscopy and its application to biomedical research." Japan.
@misc{etde_6699840,
title = {Nuclear magnetic resonance (NMR) spectroscopy and its application to biomedical research}
author = {Nakazawa, Mikio, and Imai, Shoichi}
abstractNote = {The principles of nuclear magnetic resonance (NMR) spectroscopy were explained and its application to biomedical research discussed. With /sup 31/P-NMR, it is feasible to conduct a continuous, non-invasive measurement of the contents of myocardial high-energy phosphate compounds and the intracellular pH (determined by monitoring the pH dependent shift of the inorganic phosphate peak relative to that of creatine phosphate), and to correlate them with the mechanical function. The determination of the free magnesium concentration is also possible on a similar principle to that for pH determination (the shift of MgATP peaks relative to ATP is utilized in this case). It is estimated to be 0.3 mM and was found not to be changed during ischemia. Several examples of studies including our own conducted to delineate the ischemic derangements of the myocardial energy metabolism and the effects of various interventions thereupon were illustrated. Finally a brief mention was made of the saturation transfer technique. This is the only method with which one can study the kinetics of the enzyme reactions under in vivo conditions. The application of the method for analysis of the creatine kinase reaction and the ATP synthesis was demonstrated. (author) 49 refs.}
journal = {Nippon Yakurigaku Zasshi; (Japan)}
volume = {92:1}
journal type = {AC}
place = {Japan}
year = {1988}
month = {Jul}
}