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DNA polymerase I-mediated repair of 365 nm-induced single-strand breaks in the DNA of Escherichia coli

Journal Article:

Abstract

Irradiation of closed circular phage lambda DNA in vivo at 365 nm results in the induction of single-strand breaks and alkali-labile lesions at rates of 1.1 x 10/sup -14/ and 0.2 x 10/sup -14//dalton/J/m/sup 2/, respectively. The sum of the induction rates is similar to the rate of induction of single-strand breaks plus alkali-labile lesions (1 x 10/sup -14//dalton/J/m/sup 2/) observed in the E. coli genome. Postirradiation incubation of wild-type cells in buffer results in rapid repair of the breaks (up to 80% repaired in 10 min). No repair was observed in a DNA polymerase I-deficient mutant of E.coli.
Authors:
Ley, R D; Sedita, B A; Boye, E [1] 
  1. Argonne National Lab., Ill. (USA)
Publication Date:
Mar 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-09-398196; EDB-78-125580
Resource Relation:
Journal Name: Photochem. Photobiol.; (United Kingdom); Journal Volume: 27:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; STRAND BREAKS; BIOLOGICAL REPAIR; BACTERIOPHAGES; BIOLOGICAL RADIATION EFFECTS; CHEMICAL BONDS; ESCHERICHIA COLI; MUTANTS; POLYMERASES; ULTRAVIOLET RADIATION; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; ELECTROMAGNETIC RADIATION; ENZYMES; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PARASITES; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; VIRUSES; 560112* - Radiation Effects on Biochemicals- In Microorganisms- (-1987); 560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
6685915
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PHCBA
Submitting Site:
INIS
Size:
Pages: 323-327
Announcement Date:
Sep 01, 1978

Journal Article:

Citation Formats

Ley, R D, Sedita, B A, and Boye, E. DNA polymerase I-mediated repair of 365 nm-induced single-strand breaks in the DNA of Escherichia coli. United Kingdom: N. p., 1978. Web.
Ley, R D, Sedita, B A, & Boye, E. DNA polymerase I-mediated repair of 365 nm-induced single-strand breaks in the DNA of Escherichia coli. United Kingdom.
Ley, R D, Sedita, B A, and Boye, E. 1978. "DNA polymerase I-mediated repair of 365 nm-induced single-strand breaks in the DNA of Escherichia coli." United Kingdom.
@misc{etde_6685915,
title = {DNA polymerase I-mediated repair of 365 nm-induced single-strand breaks in the DNA of Escherichia coli}
author = {Ley, R D, Sedita, B A, and Boye, E}
abstractNote = {Irradiation of closed circular phage lambda DNA in vivo at 365 nm results in the induction of single-strand breaks and alkali-labile lesions at rates of 1.1 x 10/sup -14/ and 0.2 x 10/sup -14//dalton/J/m/sup 2/, respectively. The sum of the induction rates is similar to the rate of induction of single-strand breaks plus alkali-labile lesions (1 x 10/sup -14//dalton/J/m/sup 2/) observed in the E. coli genome. Postirradiation incubation of wild-type cells in buffer results in rapid repair of the breaks (up to 80% repaired in 10 min). No repair was observed in a DNA polymerase I-deficient mutant of E.coli.}
journal = {Photochem. Photobiol.; (United Kingdom)}
volume = {27:3}
journal type = {AC}
place = {United Kingdom}
year = {1978}
month = {Mar}
}