Abstract
Aim of the invention is a procedure for the determination of phosphorylating enzyme activity by using an improved means. Cellular extracts and cell lysates, resp., are contacted with surface carriers from natural and/or synthesized polymers, containing deprotonizable groups, in presence of /sup 32/P-..gamma..-ATP and, if necessary, of ATP. The phosphorylated antibiotic is evidenced by autoradiography or any other identifying procedure. Fields of application are molecular biology, genetic engineering, biotechnology and medical special fields.
Citation Formats
Hunger, H, Behrendt, G, and Schmidt, G.
Procedures and means for determining enzyme activities. Verfahren und Mittel zur Bestimmung von Enzymaktivitaeten.
Germany: N. p.,
1988.
Web.
Hunger, H, Behrendt, G, & Schmidt, G.
Procedures and means for determining enzyme activities. Verfahren und Mittel zur Bestimmung von Enzymaktivitaeten.
Germany.
Hunger, H, Behrendt, G, and Schmidt, G.
1988.
"Procedures and means for determining enzyme activities. Verfahren und Mittel zur Bestimmung von Enzymaktivitaeten."
Germany.
@misc{etde_6675773,
title = {Procedures and means for determining enzyme activities. Verfahren und Mittel zur Bestimmung von Enzymaktivitaeten}
author = {Hunger, H, Behrendt, G, and Schmidt, G}
abstractNote = {Aim of the invention is a procedure for the determination of phosphorylating enzyme activity by using an improved means. Cellular extracts and cell lysates, resp., are contacted with surface carriers from natural and/or synthesized polymers, containing deprotonizable groups, in presence of /sup 32/P-..gamma..-ATP and, if necessary, of ATP. The phosphorylated antibiotic is evidenced by autoradiography or any other identifying procedure. Fields of application are molecular biology, genetic engineering, biotechnology and medical special fields.}
place = {Germany}
year = {1988}
month = {Feb}
}
title = {Procedures and means for determining enzyme activities. Verfahren und Mittel zur Bestimmung von Enzymaktivitaeten}
author = {Hunger, H, Behrendt, G, and Schmidt, G}
abstractNote = {Aim of the invention is a procedure for the determination of phosphorylating enzyme activity by using an improved means. Cellular extracts and cell lysates, resp., are contacted with surface carriers from natural and/or synthesized polymers, containing deprotonizable groups, in presence of /sup 32/P-..gamma..-ATP and, if necessary, of ATP. The phosphorylated antibiotic is evidenced by autoradiography or any other identifying procedure. Fields of application are molecular biology, genetic engineering, biotechnology and medical special fields.}
place = {Germany}
year = {1988}
month = {Feb}
}