You need JavaScript to view this

Procedure and means for determining aminoglucoside phosphotransferase activity. Verfahren und Mittel zur Bestimmung der Aminoglucosid-Phosphotransferaseaktivitaet

Abstract

A method and means are described for determining the activity of aminoglucoside phosphotransferase. Possible applications are genetic engineering, biotechnology and medicine. Cell lysates or cell extracts are contacted with a surface carrier on cellulose basis, in the presence of a mixture of ATP and /sup 32/P-ATP, with the phosphorylated kanamycin made visible in the solid phase by autoradiography.
Publication Date:
Feb 10, 1988
Product Type:
Patent
Report Number:
DD 254029; A1
Reference Number:
AIX-20-003409; EDB-89-001232
Resource Relation:
Patent File Date: Filed date 27 Nov 1986
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; PHOSPHOTRANSFERASES; ENZYME ACTIVITY; AMINES; ANIMAL CELLS; ANTIBIOTICS; ATP; AUTORADIOGRAPHY; BACTERIA; CELLULOSE; DECOMPOSITION; GLUCOSE; GLYCOSIDES; PHOSPHORUS 32; POLYMERS; SUBSTRATES; ALDEHYDES; ANTI-INFECTIVE AGENTS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOHYDRATES; CHEMICAL REACTIONS; DAYS LIVING RADIOISOTOPES; DRUGS; ENZYMES; HEXOSES; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; MONOSACCHARIDES; NUCLEI; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; PHOSPHORUS-GROUP TRANSFERASES; POLYSACCHARIDES; RADIOISOTOPES; SACCHARIDES; TRANSFERASES; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 090122 - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989); 550201 - Biochemistry- Tracer Techniques
OSTI ID:
6674771
Research Organizations:
Akademie der Wissenschaften der DDR, Berlin
Country of Origin:
Germany
Language:
German
Submitting Site:
INIS
Size:
Pages: 6
Announcement Date:

Citation Formats

Hunger, H D, Behrendt, G, Schmidt, G, and Merkel, D. Procedure and means for determining aminoglucoside phosphotransferase activity. Verfahren und Mittel zur Bestimmung der Aminoglucosid-Phosphotransferaseaktivitaet. Germany: N. p., 1988. Web.
Hunger, H D, Behrendt, G, Schmidt, G, & Merkel, D. Procedure and means for determining aminoglucoside phosphotransferase activity. Verfahren und Mittel zur Bestimmung der Aminoglucosid-Phosphotransferaseaktivitaet. Germany.
Hunger, H D, Behrendt, G, Schmidt, G, and Merkel, D. 1988. "Procedure and means for determining aminoglucoside phosphotransferase activity. Verfahren und Mittel zur Bestimmung der Aminoglucosid-Phosphotransferaseaktivitaet." Germany.
@misc{etde_6674771,
title = {Procedure and means for determining aminoglucoside phosphotransferase activity. Verfahren und Mittel zur Bestimmung der Aminoglucosid-Phosphotransferaseaktivitaet}
author = {Hunger, H D, Behrendt, G, Schmidt, G, and Merkel, D}
abstractNote = {A method and means are described for determining the activity of aminoglucoside phosphotransferase. Possible applications are genetic engineering, biotechnology and medicine. Cell lysates or cell extracts are contacted with a surface carrier on cellulose basis, in the presence of a mixture of ATP and /sup 32/P-ATP, with the phosphorylated kanamycin made visible in the solid phase by autoradiography.}
place = {Germany}
year = {1988}
month = {Feb}
}