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Cellulase production by a strain of Myrothecium sp

Journal Article:

Abstract

A selected strain of Myrothecium sp. was grown on various carbon sources. Cellulose was found to be the highest inducer of cellulase. CMC resulted in a moderate yield. Cellobiose resulted in a low yield. Glucose, lactose, maltose and soluble starch resulted in negligible amounts. Sucrose, glycerol and salicin were extremely unsuitable. Continuous addition of glucose or cellobiose during fermentation to cellulosic culture media reduced cellulase production, whereas addition of the entire amount of glucose or cellobiose at the beginning did not affect the enzyme production. The enzyme was precipitated from the culture filtrate with ammonium sulfate giving crude cellulase, 3854 units/g. The culture filtrate was concentrated to a one-tenth volume, 97 units/ml. The purified cellulase was prepared by dialysis 6700 units/g of enzyme precipitate.
Authors:
Publication Date:
Jan 01, 1982
Product Type:
Journal Article
Reference Number:
EDB-83-018625
Resource Relation:
Journal Name: J. Ferment. Technol.; (Japan); Journal Volume: 60:4
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; CELLULASE; BIOSYNTHESIS; ENZYME ACTIVITY; FUNGI; CELLULOLYTIC ACTIVITY; BATCH CULTURE; CELLOBIOSE; CELLULOSE; CULTURE MEDIA; GLUCOSE; GLYCEROL; LACTOSE; MALTOSE; PAPER; PURIFICATION; SACCHAROSE; STARCH; SUBSTRATES; ALCOHOLS; ALDEHYDES; CARBOHYDRATES; DISACCHARIDES; ENZYMES; GLYCOSYL HYDROLASES; HEXOSES; HYDROLASES; HYDROXY COMPOUNDS; MONOSACCHARIDES; O-GLYCOSYL HYDROLASES; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; REAGENTS; SACCHARIDES; SYNTHESIS; 550200* - Biochemistry; 550700 - Microbiology; 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
OSTI ID:
6657659
Research Organizations:
National Research Centre, Cairo, Egypt
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JFTED
Submitting Site:
HEDB
Size:
Pages: 381-383
Announcement Date:
Jan 01, 1983

Journal Article:

Citation Formats

Kassim, E A. Cellulase production by a strain of Myrothecium sp. Japan: N. p., 1982. Web.
Kassim, E A. Cellulase production by a strain of Myrothecium sp. Japan.
Kassim, E A. 1982. "Cellulase production by a strain of Myrothecium sp." Japan.
@misc{etde_6657659,
title = {Cellulase production by a strain of Myrothecium sp}
author = {Kassim, E A}
abstractNote = {A selected strain of Myrothecium sp. was grown on various carbon sources. Cellulose was found to be the highest inducer of cellulase. CMC resulted in a moderate yield. Cellobiose resulted in a low yield. Glucose, lactose, maltose and soluble starch resulted in negligible amounts. Sucrose, glycerol and salicin were extremely unsuitable. Continuous addition of glucose or cellobiose during fermentation to cellulosic culture media reduced cellulase production, whereas addition of the entire amount of glucose or cellobiose at the beginning did not affect the enzyme production. The enzyme was precipitated from the culture filtrate with ammonium sulfate giving crude cellulase, 3854 units/g. The culture filtrate was concentrated to a one-tenth volume, 97 units/ml. The purified cellulase was prepared by dialysis 6700 units/g of enzyme precipitate.}
journal = {J. Ferment. Technol.; (Japan)}
volume = {60:4}
journal type = {AC}
place = {Japan}
year = {1982}
month = {Jan}
}