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N-isopropyl-p-iodoamphetamine receptors in normal and cancerous tissue of the human lung

Journal Article:

Abstract

N-Isopropyl-p-iodoamphetamine (IMP) receptors in normal human lung tissue were characterized using a radioligand binding assay with iodine-125 IMP as the ligand. Saturation binding studies revealed the presence of two binding sites with dissociation constant (K[sub d]) values of 53[+-]2 and 4687[+-]124 nM and maximum binding capacity (Bmax) values of 7[+-]1 and 133[+-]27 pmol/mg protein (n=5) respectively. The IC[sub 50] values of various amines were as follows: IMP, 9x10[sup -5] M; propranolol, 5x10[sup -4] M; haloperidol, 6x10[sup -4] M; ketamine, 9x10[sup -3] M; dopamine, 1x10[sup -2] M. The IMP receptors of cancerous tissue obtained from human lung also had two binding sites with K[sub d] values of 54[+-]2 and 5277[+-]652 nM and Bmax values of 7[+-]1 and 103[+-]21 pmol/mg protein (n=3) respectively. There was no significant difference in binding parameters between normal and cancerous lung tissue. These results demonstrate the existence of IMP receptors and suggest that cancer does not affect the nature of IMP receptors in human lung tissue. (orig.).
Authors:
Tanaka, Eiko; Mishima, Michiaki; Kawakami, Kenzo; Sakai, Naoki; Sugiura, Naoharu; Kuno, Kenshi; [1]  Taniguchi, Takashi [2] 
  1. Kyoto Univ. (Japan). Dept. of Clinical Physiology
  2. Kyoto Pharmaceutical Univ. (Japan). Dept. of Neurobiology
Publication Date:
Apr 01, 1993
Product Type:
Journal Article
Reference Number:
DEN-93-003657; EDB-93-061148
Resource Relation:
Journal Name: European Journal of Nuclear Medicine; (Germany); Journal Volume: 20:4
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; AMPHETAMINES; RECEPTORS; LUNGS; TRACER TECHNIQUES; IN VITRO; IODINE 125; MAN; NEOPLASMS; TISSUES; AMINES; ANALEPTICS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BETA DECAY RADIOISOTOPES; BODY; CENTRAL NERVOUS SYSTEM AGENTS; DAYS LIVING RADIOISOTOPES; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MEMBRANE PROTEINS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PRIMATES; PROTEINS; RADIOISOTOPES; RESPIRATORY SYSTEM; SYMPATHOMIMETICS; VERTEBRATES; 560162* - Radionuclide Effects, Kinetics, & Toxicology- Animals, Plants, Microorganisms, & Cells
OSTI ID:
6649241
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0340-6997; CODEN: EJNMD9
Submitting Site:
DEN
Size:
Pages: 293-296
Announcement Date:

Journal Article:

Citation Formats

Tanaka, Eiko, Mishima, Michiaki, Kawakami, Kenzo, Sakai, Naoki, Sugiura, Naoharu, Kuno, Kenshi, and Taniguchi, Takashi. N-isopropyl-p-iodoamphetamine receptors in normal and cancerous tissue of the human lung. Germany: N. p., 1993. Web.
Tanaka, Eiko, Mishima, Michiaki, Kawakami, Kenzo, Sakai, Naoki, Sugiura, Naoharu, Kuno, Kenshi, & Taniguchi, Takashi. N-isopropyl-p-iodoamphetamine receptors in normal and cancerous tissue of the human lung. Germany.
Tanaka, Eiko, Mishima, Michiaki, Kawakami, Kenzo, Sakai, Naoki, Sugiura, Naoharu, Kuno, Kenshi, and Taniguchi, Takashi. 1993. "N-isopropyl-p-iodoamphetamine receptors in normal and cancerous tissue of the human lung." Germany.
@misc{etde_6649241,
title = {N-isopropyl-p-iodoamphetamine receptors in normal and cancerous tissue of the human lung}
author = {Tanaka, Eiko, Mishima, Michiaki, Kawakami, Kenzo, Sakai, Naoki, Sugiura, Naoharu, Kuno, Kenshi, and Taniguchi, Takashi}
abstractNote = {N-Isopropyl-p-iodoamphetamine (IMP) receptors in normal human lung tissue were characterized using a radioligand binding assay with iodine-125 IMP as the ligand. Saturation binding studies revealed the presence of two binding sites with dissociation constant (K[sub d]) values of 53[+-]2 and 4687[+-]124 nM and maximum binding capacity (Bmax) values of 7[+-]1 and 133[+-]27 pmol/mg protein (n=5) respectively. The IC[sub 50] values of various amines were as follows: IMP, 9x10[sup -5] M; propranolol, 5x10[sup -4] M; haloperidol, 6x10[sup -4] M; ketamine, 9x10[sup -3] M; dopamine, 1x10[sup -2] M. The IMP receptors of cancerous tissue obtained from human lung also had two binding sites with K[sub d] values of 54[+-]2 and 5277[+-]652 nM and Bmax values of 7[+-]1 and 103[+-]21 pmol/mg protein (n=3) respectively. There was no significant difference in binding parameters between normal and cancerous lung tissue. These results demonstrate the existence of IMP receptors and suggest that cancer does not affect the nature of IMP receptors in human lung tissue. (orig.).}
journal = {European Journal of Nuclear Medicine; (Germany)}
volume = {20:4}
journal type = {AC}
place = {Germany}
year = {1993}
month = {Apr}
}