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Characterization of an ethylene-related small multigene family from Lycopersicon esculentum

Thesis/Dissertation:

Abstract

cDNA clones derived from a tomato ripening-related cDNA library were used in RNA dot-blot experiments to investigate changes in the abundance of ripening related mRNAs during both natural and ethylene-induced ripening. Accumulation of the ripening-related mRNAs during natural ripening began at the time of autocatalytic ethylene production by the fruit, reached a maximum in orange fruit and declined as they became red. Analysis of the induction kinetics of these mRNAs revealed several patterns of expression as tomatoes ripened. The pTOM 13 cDNA insert was sequenced and used to identify related sequences in a tomato genomic library. 21 hybridizing genomic clones were isolated and divided into three groups of similar sequences based on their restriction maps. The DNA sequences of two of these groups of genomic clones that hybridized to pTOM 13 were determined. This allowed the identification of an incomplete pTOM 13-homologous gene, and a closely related complete gene. Nuclei were isolated from unwounded and wounded leaves and were used in run-off transcription experiments in the presence of (..cap alpha../sup 32/P)UTP. (/sup 32/P)-labelled RNA obtained from transcription experiments was used in dot-blot experiments against pTOM 13 and related genomic subclones. The results of these experiments demonstrated that the accumulation of  More>>
Authors:
Publication Date:
Jan 01, 1987
Product Type:
Thesis/Dissertation
Reference Number:
EDB-89-016493
Resource Relation:
Other Information: Thesis (Ph. D.)
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; ETHYLENE; BIOLOGICAL EFFECTS; MESSENGER-RNA; BIOSYNTHESIS; TOMATOES; DNA SEQUENCING; CELL DIFFERENTIATION; CLONE CELLS; DNA; GENES; PHOSPHORUS 32; RNA; TRACER TECHNIQUES; TRANSCRIPTION; ALKENES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CELL CULTURES; DAYS LIVING RADIOISOTOPES; FOOD; FRUITS; HYDROCARBONS; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; RADIOISOTOPES; STRUCTURAL CHEMICAL ANALYSIS; SYNTHESIS; 560300* - Chemicals Metabolism & Toxicology; 550201 - Biochemistry- Tracer Techniques
OSTI ID:
6599599
Research Organizations:
Nottingham Univ. (UK)
Country of Origin:
United Kingdom
Language:
English
Availability:
BRD-80306
Submitting Site:
JMT
Size:
Pages: 261
Announcement Date:

Thesis/Dissertation:

Citation Formats

Holdsworth, M. Characterization of an ethylene-related small multigene family from Lycopersicon esculentum. United Kingdom: N. p., 1987. Web.
Holdsworth, M. Characterization of an ethylene-related small multigene family from Lycopersicon esculentum. United Kingdom.
Holdsworth, M. 1987. "Characterization of an ethylene-related small multigene family from Lycopersicon esculentum." United Kingdom.
@misc{etde_6599599,
title = {Characterization of an ethylene-related small multigene family from Lycopersicon esculentum}
author = {Holdsworth, M}
abstractNote = {cDNA clones derived from a tomato ripening-related cDNA library were used in RNA dot-blot experiments to investigate changes in the abundance of ripening related mRNAs during both natural and ethylene-induced ripening. Accumulation of the ripening-related mRNAs during natural ripening began at the time of autocatalytic ethylene production by the fruit, reached a maximum in orange fruit and declined as they became red. Analysis of the induction kinetics of these mRNAs revealed several patterns of expression as tomatoes ripened. The pTOM 13 cDNA insert was sequenced and used to identify related sequences in a tomato genomic library. 21 hybridizing genomic clones were isolated and divided into three groups of similar sequences based on their restriction maps. The DNA sequences of two of these groups of genomic clones that hybridized to pTOM 13 were determined. This allowed the identification of an incomplete pTOM 13-homologous gene, and a closely related complete gene. Nuclei were isolated from unwounded and wounded leaves and were used in run-off transcription experiments in the presence of (..cap alpha../sup 32/P)UTP. (/sup 32/P)-labelled RNA obtained from transcription experiments was used in dot-blot experiments against pTOM 13 and related genomic subclones. The results of these experiments demonstrated that the accumulation of pTOM 13-related genes in leaves may be controlled at transcriptional and post-transcriptional levels.}
place = {United Kingdom}
year = {1987}
month = {Jan}
}