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Magnetic circular dichroism studies on microsomal aryl hydrocarbon hydroxylase: comparison with cytochrome b/sub 5/ and cytochrome P-450/sub cam/

Journal Article:

Abstract

Magnetic circular dichroism spectra are reported for the visible and near ultraviolet spectral regions of liver microsomes from dimethylbenzanthracene-treated rats. The sequential addition of NADH, dithionite, and carbon monoxide enables us to determine contributions to the magnetic circular dichroism by cytochromes b/sub 5/ and P-450, which dominate the spectra. The magnetic circular dichroism of the microsomal preparation is compared with that of purified oxidized and reduced cytochrome b/sub 5/ from pig liver and with the camphor-complexed and camphor-free oxidized, reduced, and reduced carbonmonoxy cytochrome P-450/sub cam/ from Pseudomonas putida. The magnetic circular dichroism spectra of the membrane bound cytochrome b/sub 5/ are similar to those of the purified protein, indicating that little or no alteration in the environment of the heme occurs during the isolation procedure. The soluble bacterial cytochrome P-450/sub cam/ also appears to be a suitable model for microsomal P-450, although differences in the magnetic circular dichroism intensity are observed for the two enzymes. No effect of dimethylbenzanthracene on the magnetic circular dichroism spectra of induced compared to control rat microsomes could be observed.
Publication Date:
Jan 01, 1975
Product Type:
Journal Article
Reference Number:
EDB-79-027598
Resource Relation:
Journal Name: Biochim. Biophys. Acta; (Netherlands); Journal Volume: 386
Subject:
59 BASIC BIOLOGICAL SCIENCES; CYTOCHROMES; COMPARATIVE EVALUATIONS; MAGNETIC SPECTROMETERS; BACTERIA; COENZYMES; ENZYMES; HYDROCARBONS; HYDROXYLASE; LIVER; MICROSOMES; NUCLEOTIDES; POLYCYCLIC AROMATIC HYDROCARBONS; RATS; ANIMALS; AROMATICS; BODY; CELL CONSTITUENTS; DIGESTIVE SYSTEM; GLANDS; MAMMALS; MEASURING INSTRUMENTS; MICROORGANISMS; ORGANIC COMPOUNDS; ORGANOIDS; ORGANS; OXIDOREDUCTASES; PIGMENTS; RODENTS; SPECTROMETERS; VERTEBRATES; 550300* - Cytology; 550200 - Biochemistry
OSTI ID:
6593805
Research Organizations:
Univ. of California, Berkeley
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BBACA
Submitting Site:
TIC
Size:
Pages: 87-89
Announcement Date:
Mar 01, 1979

Journal Article:

Citation Formats

Vickery, L, Salmon, A, and Sauer, K. Magnetic circular dichroism studies on microsomal aryl hydrocarbon hydroxylase: comparison with cytochrome b/sub 5/ and cytochrome P-450/sub cam/. Netherlands: N. p., 1975. Web.
Vickery, L, Salmon, A, & Sauer, K. Magnetic circular dichroism studies on microsomal aryl hydrocarbon hydroxylase: comparison with cytochrome b/sub 5/ and cytochrome P-450/sub cam/. Netherlands.
Vickery, L, Salmon, A, and Sauer, K. 1975. "Magnetic circular dichroism studies on microsomal aryl hydrocarbon hydroxylase: comparison with cytochrome b/sub 5/ and cytochrome P-450/sub cam/." Netherlands.
@misc{etde_6593805,
title = {Magnetic circular dichroism studies on microsomal aryl hydrocarbon hydroxylase: comparison with cytochrome b/sub 5/ and cytochrome P-450/sub cam/}
author = {Vickery, L, Salmon, A, and Sauer, K}
abstractNote = {Magnetic circular dichroism spectra are reported for the visible and near ultraviolet spectral regions of liver microsomes from dimethylbenzanthracene-treated rats. The sequential addition of NADH, dithionite, and carbon monoxide enables us to determine contributions to the magnetic circular dichroism by cytochromes b/sub 5/ and P-450, which dominate the spectra. The magnetic circular dichroism of the microsomal preparation is compared with that of purified oxidized and reduced cytochrome b/sub 5/ from pig liver and with the camphor-complexed and camphor-free oxidized, reduced, and reduced carbonmonoxy cytochrome P-450/sub cam/ from Pseudomonas putida. The magnetic circular dichroism spectra of the membrane bound cytochrome b/sub 5/ are similar to those of the purified protein, indicating that little or no alteration in the environment of the heme occurs during the isolation procedure. The soluble bacterial cytochrome P-450/sub cam/ also appears to be a suitable model for microsomal P-450, although differences in the magnetic circular dichroism intensity are observed for the two enzymes. No effect of dimethylbenzanthracene on the magnetic circular dichroism spectra of induced compared to control rat microsomes could be observed.}
journal = {Biochim. Biophys. Acta; (Netherlands)}
volume = {386}
journal type = {AC}
place = {Netherlands}
year = {1975}
month = {Jan}
}