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Immobilization of anaerobic thermophilic bacteria for the production of cell-free thermostable. alpha. -amylases and pullulanases

Journal Article:

Abstract

For the production of cell-free thermostable {alpha}-amylases and pullulanases various anaerobic thermophilic bacteria that belong to the genera Clostridium and Thermoanaerobacter were immobilized in calcium alginate gel beads. The entrapment of bacteria was performed in full was well as in hollow spheres. An optimal limited medium, which avoided bacterial outgrowth, was developed for the cultivation of immobilized organisms at 60deg C using 0.4% starch as substrate. Compared to non-immobilized cells these techniques allowed a significant increase (up to 5.6-fold) in the specific activities of the extracellular enzymes formed. An increase in the productivity of extracellular enzymes was observed after immobilization of bacteria in full spheres. In the case of C. thermosaccharolyticum, for instance, the productivity was raised from 90 units (U)/10{sup 12} cells up to 700 U/10{sup 12} cells. Electrophoretic analysis of the secreted proteins showed that in all cases most of the amylolytic enzymes formed were released into the culture medium. Proteins that had a molecular mass of less than 450 000 daltons could easily diffuse through the gel matrix. Cultivation of immobilized bacteria in semi-continuous and fed-batch cultures was also accompanied by an elevation in the concentration of cell-free enzymes. (orig.).
Authors:
Klingeberg, M; [1]  Vorlop, K D; [2]  Antranikian, G [3] 
  1. Goettingen Univ. (Germany, F.R.). Inst. fuer Mikrobiologie
  2. Technische Univ. Braunschweig (Germany, F.R.). Inst. fuer Technische Chemie
  3. Technische Univ. Hamburg-Harburg, Hamburg (Germany, F. R.). Arbeitsbereich Biotechnologie 1
Publication Date:
Aug 01, 1990
Product Type:
Journal Article
Reference Number:
DE-90-009778; EDB-90-153697
Resource Relation:
Journal Name: Applied Microbiology and Biotechnology; (Germany, F.R.); Journal Volume: 33:5
Subject:
09 BIOMASS FUELS; AMYLASE; PRODUCTION; BACTERIA; IMMOBILIZED CELLS; ANAEROBIC CONDITIONS; CLOSTRIDIUM; ENZYME ACTIVITY; THERMOPHILIC CONDITIONS; ENZYMES; GLYCOSYL HYDROLASES; HYDROLASES; MICROORGANISMS; O-GLYCOSYL HYDROLASES; 090900* - Biomass Fuels- Processing- (1990-)
OSTI ID:
6569522
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0175-7598; CODEN: AMBID
Submitting Site:
DE
Size:
Pages: 494-500
Announcement Date:
Nov 01, 1990

Journal Article:

Citation Formats

Klingeberg, M, Vorlop, K D, and Antranikian, G. Immobilization of anaerobic thermophilic bacteria for the production of cell-free thermostable. alpha. -amylases and pullulanases. Germany: N. p., 1990. Web.
Klingeberg, M, Vorlop, K D, & Antranikian, G. Immobilization of anaerobic thermophilic bacteria for the production of cell-free thermostable. alpha. -amylases and pullulanases. Germany.
Klingeberg, M, Vorlop, K D, and Antranikian, G. 1990. "Immobilization of anaerobic thermophilic bacteria for the production of cell-free thermostable. alpha. -amylases and pullulanases." Germany.
@misc{etde_6569522,
title = {Immobilization of anaerobic thermophilic bacteria for the production of cell-free thermostable. alpha. -amylases and pullulanases}
author = {Klingeberg, M, Vorlop, K D, and Antranikian, G}
abstractNote = {For the production of cell-free thermostable {alpha}-amylases and pullulanases various anaerobic thermophilic bacteria that belong to the genera Clostridium and Thermoanaerobacter were immobilized in calcium alginate gel beads. The entrapment of bacteria was performed in full was well as in hollow spheres. An optimal limited medium, which avoided bacterial outgrowth, was developed for the cultivation of immobilized organisms at 60deg C using 0.4% starch as substrate. Compared to non-immobilized cells these techniques allowed a significant increase (up to 5.6-fold) in the specific activities of the extracellular enzymes formed. An increase in the productivity of extracellular enzymes was observed after immobilization of bacteria in full spheres. In the case of C. thermosaccharolyticum, for instance, the productivity was raised from 90 units (U)/10{sup 12} cells up to 700 U/10{sup 12} cells. Electrophoretic analysis of the secreted proteins showed that in all cases most of the amylolytic enzymes formed were released into the culture medium. Proteins that had a molecular mass of less than 450 000 daltons could easily diffuse through the gel matrix. Cultivation of immobilized bacteria in semi-continuous and fed-batch cultures was also accompanied by an elevation in the concentration of cell-free enzymes. (orig.).}
journal = {Applied Microbiology and Biotechnology; (Germany, F.R.)}
volume = {33:5}
journal type = {AC}
place = {Germany}
year = {1990}
month = {Aug}
}