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Evaluation of the contribution of recycled urea to the synthesis of the microbial of protein in the rumen using sup 15 N labelled urea

Journal Article:

Abstract

Urea {sup 15}N was continually infused into the juglar vein for 3 days in two duodenally cannulated cows fed diets of 1:2 and 2:1 forage: concentrate. Duodenal digesta samples were taken every 3 hours, and coccygeal blood and milk were sampled twice daily. Urine was collected for 5 days starting 1 day before infusion and total feces for 3 days during infusion. Fecal samples were also taken twice daily during the 5 days of collection. Urinary excretion of {sup 15}N accounted for about 90% of that which existed from the body; whereas, feces and milk each accounted for about 5%. Recovery of {sup 15}N during the infusion period ranged from 30 to 50% of that infused. Estimates using {sup 15}N ratios, as percent of the total N passing into the duodenum, that was bacterial N, were 50% to 90% and appeared directly proportional to dry matter intake of cows. Recycled-N incorporated into rumen microbes was greater (24% verses 14% of N in bacteria passing into the duodenum) in cows fed the high concentrate than the high forage diet. Also, incorporation of recycled N into rumen microbes was higher in the lactating than the dry cow (24% verses 14%) and the  More>>
Authors:
Al-Dehneh, A M [1] 
  1. Michigan State Univ. (USA) Dept. of Animal Science
Publication Date:
Jan 01, 1990
Product Type:
Journal Article
Reference Number:
AIX-21-089961; EDB-90-167429
Resource Relation:
Journal Name: Aalam Al-Zarra (The World of Atom); (Syria); Journal Volume: 10; Other Information: Summary of Msc. thesis, Michigan State Univ., Dept. of Animal Science, USA, 1986
Subject:
60 APPLIED LIFE SCIENCES; PROTEINS; SYNTHESIS; UREA; RUMINANTS; STOMACH; NITROGEN 15; AMIDES; ANIMALS; BODY; CARBONIC ACID DERIVATIVES; DIGESTIVE SYSTEM; GASTROINTESTINAL TRACT; ISOTOPES; LIGHT NUCLEI; MAMMALS; NITROGEN ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; STABLE ISOTOPES; VERTEBRATES; 553005* - Agriculture & Food Technology- Animal Husbandry- (1987-)
OSTI ID:
6553870
Country of Origin:
Syrian Arab Republic
Language:
Arabic
Other Identifying Numbers:
Journal ID: CODEN: AAALE
Submitting Site:
INIS
Size:
Pages: 117-122
Announcement Date:

Journal Article:

Citation Formats

Al-Dehneh, A M. Evaluation of the contribution of recycled urea to the synthesis of the microbial of protein in the rumen using sup 15 N labelled urea. Syrian Arab Republic: N. p., 1990. Web.
Al-Dehneh, A M. Evaluation of the contribution of recycled urea to the synthesis of the microbial of protein in the rumen using sup 15 N labelled urea. Syrian Arab Republic.
Al-Dehneh, A M. 1990. "Evaluation of the contribution of recycled urea to the synthesis of the microbial of protein in the rumen using sup 15 N labelled urea." Syrian Arab Republic.
@misc{etde_6553870,
title = {Evaluation of the contribution of recycled urea to the synthesis of the microbial of protein in the rumen using sup 15 N labelled urea}
author = {Al-Dehneh, A M}
abstractNote = {Urea {sup 15}N was continually infused into the juglar vein for 3 days in two duodenally cannulated cows fed diets of 1:2 and 2:1 forage: concentrate. Duodenal digesta samples were taken every 3 hours, and coccygeal blood and milk were sampled twice daily. Urine was collected for 5 days starting 1 day before infusion and total feces for 3 days during infusion. Fecal samples were also taken twice daily during the 5 days of collection. Urinary excretion of {sup 15}N accounted for about 90% of that which existed from the body; whereas, feces and milk each accounted for about 5%. Recovery of {sup 15}N during the infusion period ranged from 30 to 50% of that infused. Estimates using {sup 15}N ratios, as percent of the total N passing into the duodenum, that was bacterial N, were 50% to 90% and appeared directly proportional to dry matter intake of cows. Recycled-N incorporated into rumen microbes was greater (24% verses 14% of N in bacteria passing into the duodenum) in cows fed the high concentrate than the high forage diet. Also, incorporation of recycled N into rumen microbes was higher in the lactating than the dry cow (24% verses 14%) and the flow of nitrogen from the rumen to the small intestine was greater for the concentrate than the forage diet (122.O% verses 101.0% of nitrogen intake). (author). 27 refs.}
journal = {Aalam Al-Zarra (The World of Atom); (Syria)}
volume = {10}
journal type = {AC}
place = {Syrian Arab Republic}
year = {1990}
month = {Jan}
}