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Radioautographic study of RNA synthesis in Caenorhabditis elegans (Bergerac variety) oogenesis. [Nematode]

Journal Article:

Abstract

An original method of incubation allowing the use of radioactive precursor for RNA synthesis study, is described. This in vitro incubation technique offers the advantages of: being simple (it does not require axenic culture as various authors concluded when they attempted nematode labelling); being rapid (in vivo system requires 20 to 24 hours incubation to obtain labelling); being repeatable (the ten different preparations of each of our experiments behave in a very homogeneous way). Then, this technique offers a great interest to study: the kinetic of RNA synthesis in oogonia and oocytes, and also in the rachis, specific to nematodes of which function is poorly understood; the reproduction of this hermaphroditic C. elegans as compared in the wild-type and in the thermosensitive or female sterile mutants.
Authors:
Starck, J [1] 
  1. Lyon-1 Univ., 69 - Villeurbanne (France)
Publication Date:
Nov 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-09-411160; EDB-79-022119
Resource Relation:
Journal Name: Biol. Cell.; (France); Journal Volume: 30:2
Subject:
59 BASIC BIOLOGICAL SCIENCES; NEMATODES; OOGENESIS; REPRODUCTION; OOCYTES; AUTORADIOGRAPHY; OOGONIA; RNA; BIOSYNTHESIS; INCUBATION; OVARIES; TRITIUM COMPOUNDS; URIDINE; ANIMALS; AZINES; BODY; FEMALE GENITALS; GAMETOGENESIS; GERM CELLS; GONADS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INVERTEBRATES; LABELLED COMPOUNDS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; RIBOSIDES; SYNTHESIS; URACILS; 551001* - Physiological Systems- Tracer Techniques; 550201 - Biochemistry- Tracer Techniques
OSTI ID:
6541796
Country of Origin:
France
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BICED
Submitting Site:
INIS
Size:
Pages: 181-182
Announcement Date:

Journal Article:

Citation Formats

Starck, J. Radioautographic study of RNA synthesis in Caenorhabditis elegans (Bergerac variety) oogenesis. [Nematode]. France: N. p., 1977. Web.
Starck, J. Radioautographic study of RNA synthesis in Caenorhabditis elegans (Bergerac variety) oogenesis. [Nematode]. France.
Starck, J. 1977. "Radioautographic study of RNA synthesis in Caenorhabditis elegans (Bergerac variety) oogenesis. [Nematode]." France.
@misc{etde_6541796,
title = {Radioautographic study of RNA synthesis in Caenorhabditis elegans (Bergerac variety) oogenesis. [Nematode]}
author = {Starck, J}
abstractNote = {An original method of incubation allowing the use of radioactive precursor for RNA synthesis study, is described. This in vitro incubation technique offers the advantages of: being simple (it does not require axenic culture as various authors concluded when they attempted nematode labelling); being rapid (in vivo system requires 20 to 24 hours incubation to obtain labelling); being repeatable (the ten different preparations of each of our experiments behave in a very homogeneous way). Then, this technique offers a great interest to study: the kinetic of RNA synthesis in oogonia and oocytes, and also in the rachis, specific to nematodes of which function is poorly understood; the reproduction of this hermaphroditic C. elegans as compared in the wild-type and in the thermosensitive or female sterile mutants.}
journal = {Biol. Cell.; (France)}
volume = {30:2}
journal type = {AC}
place = {France}
year = {1977}
month = {Nov}
}