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Inhibition of DNA chain elongation in Chinese hamster cells by damage localized behind the replication fork

Abstract

Chinese hamster fibroblasts were pulse labelled with 5-bromodeoxyuridine and exposed at time intervals (Tsub(i)) to near-ultraviolet (U.V.A.) light in the presence of a bisbenzimidazole derivative (Hoechst 33342). The sensitivity of the cells in terms of colony forming ability fluctuated depending on Tsub(i). Inhibition of DNA synthesis also depended on Tsub(i) and was maximal when Tsub(i)=O. Using the alkaline elution technique it was shown that the effect of a large dose of light was to inhibit both initiation and elongation of DNA chains. These effects were most pronounced for Tsub(i)=O. It is concluded that DNA damage in an active replicon can inhibit initiation of new replicons and that damage localized behind the replication fork can retard elongation of nascent DNA chains. This effect on chain elongation decreases with increased distance of the damage from the replication fork.
Authors:
Ben-Hur, E; [1]  Hagan, M P [2] 
  1. Israel Atomic Energy Commission, Beersheba. Nuclear Research Center-Negev
  2. Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
Publication Date:
May 01, 1984
Product Type:
Journal Article
Reference Number:
AIX-15-055847; EDB-84-160155
Resource Relation:
Journal Name: Int. J. Radiat. Biol. Relat. Stud. Phys., Chem. Med.; (United Kingdom); Journal Volume: 45:5
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA REPLICATION; BIOLOGICAL RADIATION EFFECTS; BENZIMIDAZOLES; BUDR; FIBROBLASTS; HAMSTERS; NEAR ULTRAVIOLET RADIATION; PHOTOLYSIS; TIME DEPENDENCE; TRACER TECHNIQUES; ANIMAL CELLS; ANIMALS; ANTIMETABOLITES; AZINES; AZOLES; BIOLOGICAL EFFECTS; BROMOURACILS; CHEMICAL REACTIONS; CONNECTIVE TISSUE CELLS; DECOMPOSITION; DRUGS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; IMIDAZOLES; ISOTOPE APPLICATIONS; MAMMALS; NUCLEIC ACID REPLICATION; NUCLEOSIDES; NUCLEOTIDES; ORGANIC BROMINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHOTOCHEMICAL REACTIONS; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; RIBOSIDES; RODENTS; SOMATIC CELLS; ULTRAVIOLET RADIATION; URACILS; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987)
OSTI ID:
6539667
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: IJRBA
Submitting Site:
HEDB
Size:
Pages: 505-514
Announcement Date:

Citation Formats

Ben-Hur, E, and Hagan, M P. Inhibition of DNA chain elongation in Chinese hamster cells by damage localized behind the replication fork. United Kingdom: N. p., 1984. Web. doi:10.1080/09553008414550711.
Ben-Hur, E, & Hagan, M P. Inhibition of DNA chain elongation in Chinese hamster cells by damage localized behind the replication fork. United Kingdom. doi:10.1080/09553008414550711.
Ben-Hur, E, and Hagan, M P. 1984. "Inhibition of DNA chain elongation in Chinese hamster cells by damage localized behind the replication fork." United Kingdom. doi:10.1080/09553008414550711. https://www.osti.gov/servlets/purl/10.1080/09553008414550711.
@misc{etde_6539667,
title = {Inhibition of DNA chain elongation in Chinese hamster cells by damage localized behind the replication fork}
author = {Ben-Hur, E, and Hagan, M P}
abstractNote = {Chinese hamster fibroblasts were pulse labelled with 5-bromodeoxyuridine and exposed at time intervals (Tsub(i)) to near-ultraviolet (U.V.A.) light in the presence of a bisbenzimidazole derivative (Hoechst 33342). The sensitivity of the cells in terms of colony forming ability fluctuated depending on Tsub(i). Inhibition of DNA synthesis also depended on Tsub(i) and was maximal when Tsub(i)=O. Using the alkaline elution technique it was shown that the effect of a large dose of light was to inhibit both initiation and elongation of DNA chains. These effects were most pronounced for Tsub(i)=O. It is concluded that DNA damage in an active replicon can inhibit initiation of new replicons and that damage localized behind the replication fork can retard elongation of nascent DNA chains. This effect on chain elongation decreases with increased distance of the damage from the replication fork.}
doi = {10.1080/09553008414550711}
journal = {Int. J. Radiat. Biol. Relat. Stud. Phys., Chem. Med.; (United Kingdom)}
volume = {45:5}
journal type = {AC}
place = {United Kingdom}
year = {1984}
month = {May}
}