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Autoradiographic study of the permeability characteristics of the small intestine

Abstract

This autoradiographic study demonstrates the distribution of a range of small solutes and macromolecules in the mucosa of the guinea-pig small intestine after intracardiac injection. The substances investigated were: /sup 14/C-urea, /sup 3/H-mannose, /sup 3/H-inulin, and /sup 125/I polyvinylpyrrolidone (PVP). Small bowel biopsies were taken at intervals from one to 60 minutes after injection and the tissues processed for autoradiography. Light microscopic examination of the autoradiographs showed that the compartmental distribution depended on the molecular size of the substances being studied. Urea and mannose, as small solutes, were uniformly distributed throughout the intravascular, extravascular, and epithelial compartments. Inulin was evenly distributed in the vessel lumen and extravascular space but there was a considerable drop in concentration in the epithelium. PVP exhibited the most marked gradients, the concentration being greatest in the vascular lumina, lower in the extravascular space, least in the epithelium. Thus there appear to be two barriers to macromolecular passage which are freely permeable to small solutes: the capillary wall and the epithelium. At a light microscopical level it was not possible to observe whether the limiting membrane of each of these barriers is the cell plasmalemmal membrane or the basement membrane. The selectivity of the epithelial barrier  More>>
Authors:
Kingham, J G.C.; Baker, J H; Loehry, C A [1] 
  1. Royal Victoria Hospital, Bournemouth (UK)
Publication Date:
Feb 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-09-401651; EDB-79-038931
Resource Relation:
Journal Name: Gut; (United Kingdom); Journal Volume: 19:2
Subject:
59 BASIC BIOLOGICAL SCIENCES; INULIN; BIOLOGICAL LOCALIZATION; MANNOSE; PVP; SMALL INTESTINE; PERMEABILITY; UREA; CAPILLARIES; CARBON 14 COMPOUNDS; CELL MEMBRANES; COMPARATIVE EVALUATIONS; COMPARTMENTS; ENDOTHELIUM; EPITHELIUM; GUINEA PIGS; IN VIVO; IODINE 125; MOLECULAR WEIGHT; MUCOUS MEMBRANES; ORGANIC IODINE COMPOUNDS; RADIONUCLIDE KINETICS; SPECIFICITY; TIME DEPENDENCE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALDEHYDES; AMIDES; ANIMALS; AZOLES; BETA DECAY RADIOISOTOPES; BLOOD SUBSTITUTES; BLOOD VESSELS; BODY; CARBOHYDRATES; CARBONIC ACID DERIVATIVES; CARDIOVASCULAR SYSTEM; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; ELECTRON CAPTURE RADIOISOTOPES; GASTROINTESTINAL TRACT; HETEROCYCLIC COMPOUNDS; HEXOSES; INTERMEDIATE MASS NUCLEI; INTESTINES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; LACTAMS; MAMMALS; MEMBRANES; MONOSACCHARIDES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC POLYMERS; ORGANS; POLYMERS; POLYSACCHARIDES; POLYVINYLS; PYRROLES; PYRROLIDONES; RADIOISOTOPES; RODENTS; SACCHARIDES; TISSUES; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques
OSTI ID:
6510331
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: GUTTA
Submitting Site:
INIS
Size:
Pages: 114-120
Announcement Date:

Citation Formats

Kingham, J G.C., Baker, J H, and Loehry, C A. Autoradiographic study of the permeability characteristics of the small intestine. United Kingdom: N. p., 1978. Web. doi:10.1136/gut.19.2.114.
Kingham, J G.C., Baker, J H, & Loehry, C A. Autoradiographic study of the permeability characteristics of the small intestine. United Kingdom. doi:10.1136/gut.19.2.114.
Kingham, J G.C., Baker, J H, and Loehry, C A. 1978. "Autoradiographic study of the permeability characteristics of the small intestine." United Kingdom. doi:10.1136/gut.19.2.114. https://www.osti.gov/servlets/purl/10.1136/gut.19.2.114.
@misc{etde_6510331,
title = {Autoradiographic study of the permeability characteristics of the small intestine}
author = {Kingham, J G.C., Baker, J H, and Loehry, C A}
abstractNote = {This autoradiographic study demonstrates the distribution of a range of small solutes and macromolecules in the mucosa of the guinea-pig small intestine after intracardiac injection. The substances investigated were: /sup 14/C-urea, /sup 3/H-mannose, /sup 3/H-inulin, and /sup 125/I polyvinylpyrrolidone (PVP). Small bowel biopsies were taken at intervals from one to 60 minutes after injection and the tissues processed for autoradiography. Light microscopic examination of the autoradiographs showed that the compartmental distribution depended on the molecular size of the substances being studied. Urea and mannose, as small solutes, were uniformly distributed throughout the intravascular, extravascular, and epithelial compartments. Inulin was evenly distributed in the vessel lumen and extravascular space but there was a considerable drop in concentration in the epithelium. PVP exhibited the most marked gradients, the concentration being greatest in the vascular lumina, lower in the extravascular space, least in the epithelium. Thus there appear to be two barriers to macromolecular passage which are freely permeable to small solutes: the capillary wall and the epithelium. At a light microscopical level it was not possible to observe whether the limiting membrane of each of these barriers is the cell plasmalemmal membrane or the basement membrane. The selectivity of the epithelial barrier was greater than that of the capillary barrier.}
doi = {10.1136/gut.19.2.114}
journal = {Gut; (United Kingdom)}
volume = {19:2}
journal type = {AC}
place = {United Kingdom}
year = {1978}
month = {Feb}
}