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Radioimmunoassay for biopterin

Journal Article:

Abstract

Specific antibodies against biopterin, neopterin, and 6,7-dimethylpterin were prepared and a new type of radioimmunoassay, to which these pterins were subjected was developed. This new type of radioimmunoassay was used to determine biopterin in human urine. Specific antiserum against biopterin did not crossreact significantly with tetrahydro-biopterin, dihydro-biopterin, neopterin, 6,7-dimethylpterin, pterin, or folic acid and showed 30% binding of biopterinyl-caproyl- ( /sup 125/I ) tyramide at a dilution of 1:800. The antisera against neopterin and dimethylpterin also showed a high specificity and did not cross-react significantly with the other pterins. The sensitivity of the radioimmunoassay was increased about ten-fold by pre-incubating the antiserum with 6,7-dimethylpterinyl-caproyl to remove antibodies against the caproic acid moiety of the biopterin conjugate. The recovery of biopterin or tetrahydro-biopterin added human urine was nearly 100% according to this type of radioimmunoassay, and the biopterin concentrations in the urine that were obtained by means of this type of radioimmunoassay showed a fairly good agreement with the values obtained by means of bioassay. The biopterin contents per milliliter of human urine showed considerable variations depending on the subjects, but those per milligram of creatinine were found to be fairly constant in normal subjects. Therefore, biopterin concentrations per milligram of  More>>
Authors:
Nagatsu, T; Yamaguchi, T; Kato, T; [1]  Sugimoto, T; Matsuura, S
  1. Tokyo Inst. of Tech. (Japan)
Publication Date:
Jun 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-12-591800; EDB-81-076360
Resource Relation:
Journal Name: Proc. Jpn. Acad.; (Japan); Journal Volume: 55
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; METABOLIC DISEASES; DIAGNOSIS; PTERIDINES; RADIOIMMUNOASSAY; ACCURACY; ANTIBODIES; CREATININE; IMMUNE REACTIONS; IMMUNE SERUMS; IODINE 125; QUANTITY RATIO; SENSITIVITY; SPECIFICITY; URINE; AZOLES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; HETEROCYCLIC COMPOUNDS; IMIDAZOLES; IMINES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; WASTES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6502319
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PJACA
Submitting Site:
INIS
Size:
Pages: 317-322
Announcement Date:
Mar 01, 1981

Journal Article:

Citation Formats

Nagatsu, T, Yamaguchi, T, Kato, T, Sugimoto, T, and Matsuura, S. Radioimmunoassay for biopterin. Japan: N. p., 1979. Web.
Nagatsu, T, Yamaguchi, T, Kato, T, Sugimoto, T, & Matsuura, S. Radioimmunoassay for biopterin. Japan.
Nagatsu, T, Yamaguchi, T, Kato, T, Sugimoto, T, and Matsuura, S. 1979. "Radioimmunoassay for biopterin." Japan.
@misc{etde_6502319,
title = {Radioimmunoassay for biopterin}
author = {Nagatsu, T, Yamaguchi, T, Kato, T, Sugimoto, T, and Matsuura, S}
abstractNote = {Specific antibodies against biopterin, neopterin, and 6,7-dimethylpterin were prepared and a new type of radioimmunoassay, to which these pterins were subjected was developed. This new type of radioimmunoassay was used to determine biopterin in human urine. Specific antiserum against biopterin did not crossreact significantly with tetrahydro-biopterin, dihydro-biopterin, neopterin, 6,7-dimethylpterin, pterin, or folic acid and showed 30% binding of biopterinyl-caproyl- ( /sup 125/I ) tyramide at a dilution of 1:800. The antisera against neopterin and dimethylpterin also showed a high specificity and did not cross-react significantly with the other pterins. The sensitivity of the radioimmunoassay was increased about ten-fold by pre-incubating the antiserum with 6,7-dimethylpterinyl-caproyl to remove antibodies against the caproic acid moiety of the biopterin conjugate. The recovery of biopterin or tetrahydro-biopterin added human urine was nearly 100% according to this type of radioimmunoassay, and the biopterin concentrations in the urine that were obtained by means of this type of radioimmunoassay showed a fairly good agreement with the values obtained by means of bioassay. The biopterin contents per milliliter of human urine showed considerable variations depending on the subjects, but those per milligram of creatinine were found to be fairly constant in normal subjects. Therefore, biopterin concentrations per milligram of creatinine in human urine may be a good indicator of biopterin metabolism in clinical chemistry. This new type of radioimmunoassay is simple, highly specific, and reproducible. Therefore, it may be very useful for the screening of atypical phenylketonuria due to biopterin deficiency and also for the study of the pathogenesis of various biopterin metabolic diseases.}
journal = {Proc. Jpn. Acad.; (Japan)}
volume = {55}
journal type = {AC}
place = {Japan}
year = {1979}
month = {Jun}
}