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Radioimmunoassays and 2-site immunoradiometric 'sandwich' assays: basic principles

Journal Article:

Abstract

The 'sandwich' or noncompetitive reagent-excess, 2-site immunoradiometric assay (2-site IRMA), ELISA, USERIA, and related techniques, have several advantages compared with the traditional or competitive radioimmunoassays. IRMAs can provide improved sensitivity and specificity. However, IRMAs present some practical problems with nonspecific binding, increased consumption of antibody, biphasic dose response curve, (high dose hook effect), and may require special techniques for dose response curve analysis. We anticipate considerable growth in the popularity and importance of 2-site IRMA.
Authors:
Publication Date:
Oct 01, 1988
Product Type:
Journal Article
Reference Number:
JPN-89-004066; EDB-89-042154
Resource Relation:
Journal Name: Radioisotopes (Tokyo); (Japan); Journal Volume: 37:10
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; RADIOIMMUNOASSAY; RADIOMETRIC ANALYSIS; ANTIGEN-ANTIBODY REACTIONS; COMPARATIVE EVALUATIONS; IMMUNE REACTIONS; MEASURING METHODS; SENSITIVITY; SPECIFICITY; CHEMICAL ANALYSIS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; QUANTITATIVE CHEMICAL ANALYSIS; RADIOASSAY; RADIOIMMUNOLOGY; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6499340
Research Organizations:
National Inst. of Child Health and Human Development, Bethesda, MD (USA)
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: RAISA
Submitting Site:
JPN
Size:
Pages: 590-594
Announcement Date:
Feb 01, 1989

Journal Article:

Citation Formats

Rodbard, D. Radioimmunoassays and 2-site immunoradiometric 'sandwich' assays: basic principles. Japan: N. p., 1988. Web.
Rodbard, D. Radioimmunoassays and 2-site immunoradiometric 'sandwich' assays: basic principles. Japan.
Rodbard, D. 1988. "Radioimmunoassays and 2-site immunoradiometric 'sandwich' assays: basic principles." Japan.
@misc{etde_6499340,
title = {Radioimmunoassays and 2-site immunoradiometric 'sandwich' assays: basic principles}
author = {Rodbard, D}
abstractNote = {The 'sandwich' or noncompetitive reagent-excess, 2-site immunoradiometric assay (2-site IRMA), ELISA, USERIA, and related techniques, have several advantages compared with the traditional or competitive radioimmunoassays. IRMAs can provide improved sensitivity and specificity. However, IRMAs present some practical problems with nonspecific binding, increased consumption of antibody, biphasic dose response curve, (high dose hook effect), and may require special techniques for dose response curve analysis. We anticipate considerable growth in the popularity and importance of 2-site IRMA.}
journal = {Radioisotopes (Tokyo); (Japan)}
volume = {37:10}
journal type = {AC}
place = {Japan}
year = {1988}
month = {Oct}
}