You need JavaScript to view this

Evaluation of two reverse passive haemagglutination techniques and a solid-phase radioimmunoassay for detection of hepatitis B surface antigen

Journal Article:

Abstract

The sensitivity and specificity of two commercially available reverse passive haemagglutination tests (Hepatest and Raphadex B) for the detection of hepatitis B surface antigen, were compared with the most widely used radioimmunoassay (Ausria II-125). A selected group of 282 sera were tested: these included the Australian hepatitis B reference panel, and a batch of 257 sera collected from patients with acute hepatitis B, chronic carriers of hepatitis B surface antigen and two populations in which hepatitis B virus infection is known to be endemic. The two reverse passive haemagglutination techniques were of comparable sensitivity but slightly less sensitive than radioimmunoassay. While radioimmunoassay still remains the test of choice for blood transfusion services, the reverse passive haemagglutination techniques are of great value for smaller laboratories and for field studies because of their longer shelf life, the absence of radioactive reagents and the lack of need to acquire a gammacounter.
Authors:
Zhuang, H; [1]  Coulepis, A G; Gust, I D [2] 
  1. Beijing Medical College (China)
  2. Fairfield Hospital for Communicable Diseases, Melbourne (Australia)
Publication Date:
Aug 01, 1972
Product Type:
Journal Article
Reference Number:
AIX-14-719751; EDB-83-052276
Resource Relation:
Journal Name: Aust. J. Med. Lab. Sci.; (Australia); Journal Volume: 3:3
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; HEPATITIS; DIAGNOSIS; VIRUSES; RADIOIMMUNOASSAY; ANTIGENS; BLOOD SERUM; COMPARATIVE EVALUATIONS; HEMAGGLUTININS; INFECTIOUS DISEASES; SENSITIVITY; AGGLUTININS; ANTIBODIES; DIGESTIVE SYSTEM DISEASES; DISEASES; ISOTOPE APPLICATIONS; MICROORGANISMS; PARASITES; RADIOASSAY; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6491929
Country of Origin:
Australia
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: AJMLD
Submitting Site:
HEDB
Size:
Pages: 93-97
Announcement Date:
Jan 01, 1983

Journal Article:

Citation Formats

Zhuang, H, Coulepis, A G, and Gust, I D. Evaluation of two reverse passive haemagglutination techniques and a solid-phase radioimmunoassay for detection of hepatitis B surface antigen. Australia: N. p., 1972. Web.
Zhuang, H, Coulepis, A G, & Gust, I D. Evaluation of two reverse passive haemagglutination techniques and a solid-phase radioimmunoassay for detection of hepatitis B surface antigen. Australia.
Zhuang, H, Coulepis, A G, and Gust, I D. 1972. "Evaluation of two reverse passive haemagglutination techniques and a solid-phase radioimmunoassay for detection of hepatitis B surface antigen." Australia.
@misc{etde_6491929,
title = {Evaluation of two reverse passive haemagglutination techniques and a solid-phase radioimmunoassay for detection of hepatitis B surface antigen}
author = {Zhuang, H, Coulepis, A G, and Gust, I D}
abstractNote = {The sensitivity and specificity of two commercially available reverse passive haemagglutination tests (Hepatest and Raphadex B) for the detection of hepatitis B surface antigen, were compared with the most widely used radioimmunoassay (Ausria II-125). A selected group of 282 sera were tested: these included the Australian hepatitis B reference panel, and a batch of 257 sera collected from patients with acute hepatitis B, chronic carriers of hepatitis B surface antigen and two populations in which hepatitis B virus infection is known to be endemic. The two reverse passive haemagglutination techniques were of comparable sensitivity but slightly less sensitive than radioimmunoassay. While radioimmunoassay still remains the test of choice for blood transfusion services, the reverse passive haemagglutination techniques are of great value for smaller laboratories and for field studies because of their longer shelf life, the absence of radioactive reagents and the lack of need to acquire a gammacounter.}
journal = {Aust. J. Med. Lab. Sci.; (Australia)}
volume = {3:3}
journal type = {AC}
place = {Australia}
year = {1972}
month = {Aug}
}