Abstract
..beta..-Galactosidase of E. coli origin was immobilized in the form of beads by the radiopolymerization of different combinations of monomers using a gamma irradiation technique. With the dialysed enzyme, recoveries of over 300 % could be obtained on suitable monomer combinations containing magnesium and sodium acrylates. The recovery of the enzyme also depended on the irradiation time. The immobilized enzyme had better pH and temperature stability and was less affected by the presence of metal ions in the medium, compared to the native enzyme. The optimum pH and temperatures of the immobilized enzyme were different from those of the native enzyme and were 7.0 to 7.5 and 50 deg C respectively. The immobilized enzyme was used in a column for the continuous determination of lactose with a standard type autoanalyser. Good linearity could be observed even up to 3% lactose in the sample.
Amarakone, S P;
[1]
Hayashi, Toru;
Kawashima, Koji
- Ceylon Inst. of Scientific and Industrial Research, Colombo (Sri Lanka)
Citation Formats
Amarakone, S P, Hayashi, Toru, and Kawashima, Koji.
Studies on the preparation of immobilized enzymes by radio-polymerization, 10. Preparation of. beta. -galactosidase and its utilization for the continuous determination of lactose. [Gamma radiation].
Japan: N. p.,
1983.
Web.
Amarakone, S P, Hayashi, Toru, & Kawashima, Koji.
Studies on the preparation of immobilized enzymes by radio-polymerization, 10. Preparation of. beta. -galactosidase and its utilization for the continuous determination of lactose. [Gamma radiation].
Japan.
Amarakone, S P, Hayashi, Toru, and Kawashima, Koji.
1983.
"Studies on the preparation of immobilized enzymes by radio-polymerization, 10. Preparation of. beta. -galactosidase and its utilization for the continuous determination of lactose. [Gamma radiation]."
Japan.
@misc{etde_6491305,
title = {Studies on the preparation of immobilized enzymes by radio-polymerization, 10. Preparation of. beta. -galactosidase and its utilization for the continuous determination of lactose. [Gamma radiation]}
author = {Amarakone, S P, Hayashi, Toru, and Kawashima, Koji}
abstractNote = {..beta..-Galactosidase of E. coli origin was immobilized in the form of beads by the radiopolymerization of different combinations of monomers using a gamma irradiation technique. With the dialysed enzyme, recoveries of over 300 % could be obtained on suitable monomer combinations containing magnesium and sodium acrylates. The recovery of the enzyme also depended on the irradiation time. The immobilized enzyme had better pH and temperature stability and was less affected by the presence of metal ions in the medium, compared to the native enzyme. The optimum pH and temperatures of the immobilized enzyme were different from those of the native enzyme and were 7.0 to 7.5 and 50 deg C respectively. The immobilized enzyme was used in a column for the continuous determination of lactose with a standard type autoanalyser. Good linearity could be observed even up to 3% lactose in the sample.}
journal = []
volume = {42}
journal type = {AC}
place = {Japan}
year = {1983}
month = {Mar}
}
title = {Studies on the preparation of immobilized enzymes by radio-polymerization, 10. Preparation of. beta. -galactosidase and its utilization for the continuous determination of lactose. [Gamma radiation]}
author = {Amarakone, S P, Hayashi, Toru, and Kawashima, Koji}
abstractNote = {..beta..-Galactosidase of E. coli origin was immobilized in the form of beads by the radiopolymerization of different combinations of monomers using a gamma irradiation technique. With the dialysed enzyme, recoveries of over 300 % could be obtained on suitable monomer combinations containing magnesium and sodium acrylates. The recovery of the enzyme also depended on the irradiation time. The immobilized enzyme had better pH and temperature stability and was less affected by the presence of metal ions in the medium, compared to the native enzyme. The optimum pH and temperatures of the immobilized enzyme were different from those of the native enzyme and were 7.0 to 7.5 and 50 deg C respectively. The immobilized enzyme was used in a column for the continuous determination of lactose with a standard type autoanalyser. Good linearity could be observed even up to 3% lactose in the sample.}
journal = []
volume = {42}
journal type = {AC}
place = {Japan}
year = {1983}
month = {Mar}
}