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Radio-iodination of a rabbit fibrinogen by the chloramine-T method

Abstract

A method for radio-iodination of fibrinogen using chloramine-T has been described. Samples of greater than 90% clottable counts were obtained. Electrophoretic mobility and immunodiffusion showed that the entire radioactivity was present in the fibrinogen band. In vivo studies on the turnover of this labelled product in rabbits showed a half-life of 52.8 to 61.7 hrs in two batches of animals. The results compare very well with the reported results obtained from fibrinogen labelled with radioactive iodine by the iodine-monochloride method. The advantages of the new method have been pointed out.
Authors:
Moza, A K; Kumar, M; Belavalgidad, M I; Sapru, R P [1] 
  1. Post-Graduate Inst. of Medical Education and Research, Chandigarh (India). Dept. of Experimental Medicine
Publication Date:
Jan 01, 1974
Product Type:
Journal Article
Reference Number:
AIX-09-414049; EDB-79-043385
Resource Relation:
Journal Name: Bull. Postgrad. Inst. Med. Educ. Res., Chandigarh; (India); Journal Volume: 8:3
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; FIBRINOGEN; LABELLING; IODINE 125; RADIOCHEMISTRY; BIOLOGICAL HALF-LIFE; CARRIER-FREE ISOTOPES; CHLORAMINES; LABELLED COMPOUNDS; RABBITS; RADIONUCLIDE KINETICS; RADIOPHARMACEUTICALS; AMINES; ANIMALS; BETA DECAY RADIOISOTOPES; BLOOD COAGULATION FACTORS; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; GLOBULINS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; PROTEINS; RADIOACTIVE MATERIALS; RADIOISOTOPES; VERTEBRATES; 400102* - Chemical & Spectral Procedures
OSTI ID:
6488225
Country of Origin:
India
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BPIRD
Submitting Site:
INIS
Size:
Pages: 121-123
Announcement Date:
Jan 01, 1979

Citation Formats

Moza, A K, Kumar, M, Belavalgidad, M I, and Sapru, R P. Radio-iodination of a rabbit fibrinogen by the chloramine-T method. India: N. p., 1974. Web.
Moza, A K, Kumar, M, Belavalgidad, M I, & Sapru, R P. Radio-iodination of a rabbit fibrinogen by the chloramine-T method. India.
Moza, A K, Kumar, M, Belavalgidad, M I, and Sapru, R P. 1974. "Radio-iodination of a rabbit fibrinogen by the chloramine-T method." India.
@misc{etde_6488225,
title = {Radio-iodination of a rabbit fibrinogen by the chloramine-T method}
author = {Moza, A K, Kumar, M, Belavalgidad, M I, and Sapru, R P}
abstractNote = {A method for radio-iodination of fibrinogen using chloramine-T has been described. Samples of greater than 90% clottable counts were obtained. Electrophoretic mobility and immunodiffusion showed that the entire radioactivity was present in the fibrinogen band. In vivo studies on the turnover of this labelled product in rabbits showed a half-life of 52.8 to 61.7 hrs in two batches of animals. The results compare very well with the reported results obtained from fibrinogen labelled with radioactive iodine by the iodine-monochloride method. The advantages of the new method have been pointed out.}
journal = []
volume = {8:3}
journal type = {AC}
place = {India}
year = {1974}
month = {Jan}
}