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Evaluation of immunoradiometric and ELISA versions of a microtitre plate assay for Bacillus anthracis spores

Journal Article:

Abstract

Solid-phase indirectly-labelled antibody assays for Bacillus anthracis spores heat-fixed on polystyrene microtitre plates were compared as immunoradiometric assay (IRMA) and enzyme-linked immunosorbent assay (ELISA) versions. Signal-to-noise ratios were usually higher in the IRMA than in the ELISA performed under parallel conditions but replicates were more varied in the IRMA. The antigen detection threshold and resolution limit calculated after regression analysis were broadly comparable in the 2 types of assay.
Authors:
Phillips, A P; Martin, K L; Cross, N L; [1]  Drake, R G [2] 
  1. Chemical Defence Experimental Establishment, Porton (UK)
  2. Glasgow Univ. (UK). Inst. of Biochemistry
Publication Date:
May 11, 1984
Product Type:
Journal Article
Reference Number:
AIX-15-056025; EDB-84-170336
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 70:1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; BACILLUS; RADIOMETRIC ANALYSIS; SENSITIVITY; ANTIBODIES; BACKGROUND NOISE; BACTERIAL SPORES; COMPARATIVE EVALUATIONS; IMMUNOLOGY; POLYSTYRENE; QUANTITATIVE CHEMICAL ANALYSIS; SUBSTRATES; BACTERIA; CHEMICAL ANALYSIS; MATERIALS; MICROORGANISMS; NOISE; ORGANIC COMPOUNDS; ORGANIC POLYMERS; PETROCHEMICALS; PETROLEUM PRODUCTS; PLASTICS; POLYMERS; POLYOLEFINS; POLYVINYLS; SPORES; SYNTHETIC MATERIALS; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6480194
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
HEDB
Size:
Pages: 75-81
Announcement Date:

Journal Article:

Citation Formats

Phillips, A P, Martin, K L, Cross, N L, and Drake, R G. Evaluation of immunoradiometric and ELISA versions of a microtitre plate assay for Bacillus anthracis spores. Netherlands: N. p., 1984. Web.
Phillips, A P, Martin, K L, Cross, N L, & Drake, R G. Evaluation of immunoradiometric and ELISA versions of a microtitre plate assay for Bacillus anthracis spores. Netherlands.
Phillips, A P, Martin, K L, Cross, N L, and Drake, R G. 1984. "Evaluation of immunoradiometric and ELISA versions of a microtitre plate assay for Bacillus anthracis spores." Netherlands.
@misc{etde_6480194,
title = {Evaluation of immunoradiometric and ELISA versions of a microtitre plate assay for Bacillus anthracis spores}
author = {Phillips, A P, Martin, K L, Cross, N L, and Drake, R G}
abstractNote = {Solid-phase indirectly-labelled antibody assays for Bacillus anthracis spores heat-fixed on polystyrene microtitre plates were compared as immunoradiometric assay (IRMA) and enzyme-linked immunosorbent assay (ELISA) versions. Signal-to-noise ratios were usually higher in the IRMA than in the ELISA performed under parallel conditions but replicates were more varied in the IRMA. The antigen detection threshold and resolution limit calculated after regression analysis were broadly comparable in the 2 types of assay.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {70:1}
journal type = {AC}
place = {Netherlands}
year = {1984}
month = {May}
}