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Radioassays for quantitation of intact complement proteins C2 and B in human serum

Journal Article:

Abstract

Availability of polyclonal and monoclonal antibodies recognizing determinants on the major cleavage fragments of complement proteins C2 and B enabled development of sensitive radioassays which can be used to quantitate the intact proteins in human sera. Changes in C2 and B concentrations indicative of classical or alternative pathway activation, or both, were seen in normal serum after incubation with complement activators. The authors determined the normal range of C2 concentration to be 11-35 ..mu..g/ml in 32 healthy individuals, and that of protein B to be 74-286 ..mu..g/ml. Sera from patients with systemic lupus erythematosus (SLE), septic shock, infections, and following orthopedic surgery were then assayed. Mean protein B concentration was significantly higher in SLE sera and in the infected and post-operative sera, and the mean C2 concentration in the septic shock group was significantly lower than the mean of healthy individuals. Intact C2 was not detected in known C2-deficient individuals. These assays allow parallel quantitation of the structurally and functionally homologous proteins of the classical (C2) and alternative (B) pathways, which is of interest in patients with genetic and acquired hypocomplementemia. 22 refs.; 3 figs.
Publication Date:
May 25, 1988
Product Type:
Journal Article
Reference Number:
AIX-20-026623; EDB-89-049405
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 110:1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; PROTEINS; RADIOIMMUNOASSAY; BLOOD SERUM; INFECTIOUS DISEASES; SEPTICEMIA; SKIN DISEASES; DISEASES; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; ORGANIC COMPOUNDS; RADIOASSAY; RADIOIMMUNOLOGY; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6466939
Research Organizations:
Alabama Univ., Birmingham (USA)
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
INIS
Size:
Pages: 55-62
Announcement Date:

Journal Article:

Citation Formats

Oglesby, T J, Ueda, A, and Volanakis, J E. Radioassays for quantitation of intact complement proteins C2 and B in human serum. Netherlands: N. p., 1988. Web.
Oglesby, T J, Ueda, A, & Volanakis, J E. Radioassays for quantitation of intact complement proteins C2 and B in human serum. Netherlands.
Oglesby, T J, Ueda, A, and Volanakis, J E. 1988. "Radioassays for quantitation of intact complement proteins C2 and B in human serum." Netherlands.
@misc{etde_6466939,
title = {Radioassays for quantitation of intact complement proteins C2 and B in human serum}
author = {Oglesby, T J, Ueda, A, and Volanakis, J E}
abstractNote = {Availability of polyclonal and monoclonal antibodies recognizing determinants on the major cleavage fragments of complement proteins C2 and B enabled development of sensitive radioassays which can be used to quantitate the intact proteins in human sera. Changes in C2 and B concentrations indicative of classical or alternative pathway activation, or both, were seen in normal serum after incubation with complement activators. The authors determined the normal range of C2 concentration to be 11-35 ..mu..g/ml in 32 healthy individuals, and that of protein B to be 74-286 ..mu..g/ml. Sera from patients with systemic lupus erythematosus (SLE), septic shock, infections, and following orthopedic surgery were then assayed. Mean protein B concentration was significantly higher in SLE sera and in the infected and post-operative sera, and the mean C2 concentration in the septic shock group was significantly lower than the mean of healthy individuals. Intact C2 was not detected in known C2-deficient individuals. These assays allow parallel quantitation of the structurally and functionally homologous proteins of the classical (C2) and alternative (B) pathways, which is of interest in patients with genetic and acquired hypocomplementemia. 22 refs.; 3 figs.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {110:1}
journal type = {AC}
place = {Netherlands}
year = {1988}
month = {May}
}