Abstract
D-vitamin metabolites have been isolated from human serum employing chromatographic techniques. The serum carrier protein for vitamin D (DBP) was first isolated by immunosorbent chromatography. Lipid ligands associated with DBP were then extracted with hexane and separated by high pressure liquid chromatography (HPLC). Detection of vitamin D metabolites by their absorbance of ultraviolet light is not sufficiently sensitive to monitor all vitamin D derivatives from a few millilitres of serum. Therefore, further analyses are necessary to quantitative these compounds. We have begun to develop a mass spectrometric method to achieve a reliable, quantitative procedure. As a first step towards this goal a number of pure samples of vitamin D compounds have been studied in a time-of-flight mass spectrometer based on fast heavy ion induced desorption. All vitamin D compounds examined could be detected and identified by their molecular ion and fragment spectra.
Fohlman, J;
Peterson, P A;
[1]
Kamensky, I;
Hakansson, P;
Sundqvist, B
[2]
- Uppsala Univ. (Sweden). Dept. of Cell Research
- Tandemacceleratorlaboratoriet, Uppsala (Sweden)
Citation Formats
Fohlman, J, Peterson, P A, Kamensky, I, Hakansson, P, and Sundqvist, B.
Vitamin D-metabolites from human plasma and mass spectrometric analysis by fast heavy ion induced desorption.
Netherlands: N. p.,
1982.
Web.
Fohlman, J, Peterson, P A, Kamensky, I, Hakansson, P, & Sundqvist, B.
Vitamin D-metabolites from human plasma and mass spectrometric analysis by fast heavy ion induced desorption.
Netherlands.
Fohlman, J, Peterson, P A, Kamensky, I, Hakansson, P, and Sundqvist, B.
1982.
"Vitamin D-metabolites from human plasma and mass spectrometric analysis by fast heavy ion induced desorption."
Netherlands.
@misc{etde_6456364,
title = {Vitamin D-metabolites from human plasma and mass spectrometric analysis by fast heavy ion induced desorption}
author = {Fohlman, J, Peterson, P A, Kamensky, I, Hakansson, P, and Sundqvist, B}
abstractNote = {D-vitamin metabolites have been isolated from human serum employing chromatographic techniques. The serum carrier protein for vitamin D (DBP) was first isolated by immunosorbent chromatography. Lipid ligands associated with DBP were then extracted with hexane and separated by high pressure liquid chromatography (HPLC). Detection of vitamin D metabolites by their absorbance of ultraviolet light is not sufficiently sensitive to monitor all vitamin D derivatives from a few millilitres of serum. Therefore, further analyses are necessary to quantitative these compounds. We have begun to develop a mass spectrometric method to achieve a reliable, quantitative procedure. As a first step towards this goal a number of pure samples of vitamin D compounds have been studied in a time-of-flight mass spectrometer based on fast heavy ion induced desorption. All vitamin D compounds examined could be detected and identified by their molecular ion and fragment spectra.}
journal = []
volume = {198:1}
place = {Netherlands}
year = {1982}
month = {Jul}
}
title = {Vitamin D-metabolites from human plasma and mass spectrometric analysis by fast heavy ion induced desorption}
author = {Fohlman, J, Peterson, P A, Kamensky, I, Hakansson, P, and Sundqvist, B}
abstractNote = {D-vitamin metabolites have been isolated from human serum employing chromatographic techniques. The serum carrier protein for vitamin D (DBP) was first isolated by immunosorbent chromatography. Lipid ligands associated with DBP were then extracted with hexane and separated by high pressure liquid chromatography (HPLC). Detection of vitamin D metabolites by their absorbance of ultraviolet light is not sufficiently sensitive to monitor all vitamin D derivatives from a few millilitres of serum. Therefore, further analyses are necessary to quantitative these compounds. We have begun to develop a mass spectrometric method to achieve a reliable, quantitative procedure. As a first step towards this goal a number of pure samples of vitamin D compounds have been studied in a time-of-flight mass spectrometer based on fast heavy ion induced desorption. All vitamin D compounds examined could be detected and identified by their molecular ion and fragment spectra.}
journal = []
volume = {198:1}
place = {Netherlands}
year = {1982}
month = {Jul}
}