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Incomplete separation of radioiodinated thyroid hormones in serum using specific antibodies

Journal Article:

Abstract

Alkaline Sephadex G-25 columns were used to separate labelled 3,5,3',5'-thyroxine, 3,5,3'-triiodothyronine, 3,3',5'-triiodothyronine and 3,3'-diiodothyronine from the serum binding proteins followed by a quantitative elution of each hormone by coupling to its respective antibody. It is shown that although these antibodies (diluted 1:1500-1:100 000) in our radioimmunoassays are highly specific they show a high degree of non-specific binding when they are used in the concentrations necessary to get a maximal recovery of the hormones in column separating experiments.
Authors:
Perrild, H; Skovsted, L; Korsgaard Christensen, L [1] 
  1. Department of Internal Medicine and Endocrinology, Herlev Hospital, DK-2730 Herlev, Denmark
Publication Date:
Jan 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-12-605017; EDB-81-069892
Resource Relation:
Journal Name: Acta Endocrinol.; (Denmark); Journal Volume: 95
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; THYROID HORMONES; SEPARATION PROCESSES; ANTIBODIES; BLOOD SERUM; IODINE 125; LABELLED COMPOUNDS; RADIOIMMUNOASSAY; SPECIFICITY; THYROID; BETA DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE GLANDS; GLANDS; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANS; PEPTIDE HORMONES; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6443606
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ACENA
Submitting Site:
INIS
Size:
Pages: 485-488
Announcement Date:

Journal Article:

Citation Formats

Perrild, H, Skovsted, L, and Korsgaard Christensen, L. Incomplete separation of radioiodinated thyroid hormones in serum using specific antibodies. Denmark: N. p., 1980. Web.
Perrild, H, Skovsted, L, & Korsgaard Christensen, L. Incomplete separation of radioiodinated thyroid hormones in serum using specific antibodies. Denmark.
Perrild, H, Skovsted, L, and Korsgaard Christensen, L. 1980. "Incomplete separation of radioiodinated thyroid hormones in serum using specific antibodies." Denmark.
@misc{etde_6443606,
title = {Incomplete separation of radioiodinated thyroid hormones in serum using specific antibodies}
author = {Perrild, H, Skovsted, L, and Korsgaard Christensen, L}
abstractNote = {Alkaline Sephadex G-25 columns were used to separate labelled 3,5,3',5'-thyroxine, 3,5,3'-triiodothyronine, 3,3',5'-triiodothyronine and 3,3'-diiodothyronine from the serum binding proteins followed by a quantitative elution of each hormone by coupling to its respective antibody. It is shown that although these antibodies (diluted 1:1500-1:100 000) in our radioimmunoassays are highly specific they show a high degree of non-specific binding when they are used in the concentrations necessary to get a maximal recovery of the hormones in column separating experiments.}
journal = {Acta Endocrinol.; (Denmark)}
volume = {95}
journal type = {AC}
place = {Denmark}
year = {1980}
month = {Jan}
}