Abstract
The synthesis of (/sup 3/H) reserpine of high specific activity is described. The accumulation of radioactivity in peripheral sympathetically innervated organs and in the brain after intravenous injection of (/sup 3/H) reserpine to rats was measured biochemically and its localization studied by light-microscopic autoradiography. In most of the organs and tissues investigated minute quantities of (/sup 3/H) reserpine persisted up to 10 days after injection. By autoradiography, it was observed that silver grains were unevenly distributed in various brain regions and peripheral organs 18 h and up to 10 days after administration of (/sup 3/H) reserpine. Pretreating (but not post-treating) animals with non-radioactive reserpine prevented by up to 80% the accumulation of radiolabel and abolished to a great extent the autoradiographic localization. The fact that the persistently bound radiolabel is confined to monoaminergic neurons suggests that it is irreversibly bound to its target site, the amine-storing vesicle. Support for this interpretation comes from studies demonstrating a fast anterograde axonal transport of (/sup 3/H) reserpine in the nigrostriatal tract after intranigral injection of the radiolabel. These findings demonstrate that (/sup 3/H) reserpine of high specific activity is a useful tool in studies designed to map monoaminergic pathways in the brain and
More>>
Richards, J G;
Da Prada, M;
Wuersch, J;
Lorez, H P;
Pieri, L
[1]
- Hoffmann-La Roche (F.) and Co., Basel (Switzerland)
Citation Formats
Richards, J G, Da Prada, M, Wuersch, J, Lorez, H P, and Pieri, L.
Mapping monoaminergic neurons with (/sup 3/H) reserpine by autoradiography.
United Kingdom: N. p.,
1979.
Web.
doi:10.1016/0306-4522(79)90177-5.
Richards, J G, Da Prada, M, Wuersch, J, Lorez, H P, & Pieri, L.
Mapping monoaminergic neurons with (/sup 3/H) reserpine by autoradiography.
United Kingdom.
https://doi.org/10.1016/0306-4522(79)90177-5
Richards, J G, Da Prada, M, Wuersch, J, Lorez, H P, and Pieri, L.
1979.
"Mapping monoaminergic neurons with (/sup 3/H) reserpine by autoradiography."
United Kingdom.
https://doi.org/10.1016/0306-4522(79)90177-5.
@misc{etde_6409475,
title = {Mapping monoaminergic neurons with (/sup 3/H) reserpine by autoradiography}
author = {Richards, J G, Da Prada, M, Wuersch, J, Lorez, H P, and Pieri, L}
abstractNote = {The synthesis of (/sup 3/H) reserpine of high specific activity is described. The accumulation of radioactivity in peripheral sympathetically innervated organs and in the brain after intravenous injection of (/sup 3/H) reserpine to rats was measured biochemically and its localization studied by light-microscopic autoradiography. In most of the organs and tissues investigated minute quantities of (/sup 3/H) reserpine persisted up to 10 days after injection. By autoradiography, it was observed that silver grains were unevenly distributed in various brain regions and peripheral organs 18 h and up to 10 days after administration of (/sup 3/H) reserpine. Pretreating (but not post-treating) animals with non-radioactive reserpine prevented by up to 80% the accumulation of radiolabel and abolished to a great extent the autoradiographic localization. The fact that the persistently bound radiolabel is confined to monoaminergic neurons suggests that it is irreversibly bound to its target site, the amine-storing vesicle. Support for this interpretation comes from studies demonstrating a fast anterograde axonal transport of (/sup 3/H) reserpine in the nigrostriatal tract after intranigral injection of the radiolabel. These findings demonstrate that (/sup 3/H) reserpine of high specific activity is a useful tool in studies designed to map monoaminergic pathways in the brain and to further characterize amine-storing mechanisms.}
doi = {10.1016/0306-4522(79)90177-5}
journal = []
volume = {4:7}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Jan}
}
title = {Mapping monoaminergic neurons with (/sup 3/H) reserpine by autoradiography}
author = {Richards, J G, Da Prada, M, Wuersch, J, Lorez, H P, and Pieri, L}
abstractNote = {The synthesis of (/sup 3/H) reserpine of high specific activity is described. The accumulation of radioactivity in peripheral sympathetically innervated organs and in the brain after intravenous injection of (/sup 3/H) reserpine to rats was measured biochemically and its localization studied by light-microscopic autoradiography. In most of the organs and tissues investigated minute quantities of (/sup 3/H) reserpine persisted up to 10 days after injection. By autoradiography, it was observed that silver grains were unevenly distributed in various brain regions and peripheral organs 18 h and up to 10 days after administration of (/sup 3/H) reserpine. Pretreating (but not post-treating) animals with non-radioactive reserpine prevented by up to 80% the accumulation of radiolabel and abolished to a great extent the autoradiographic localization. The fact that the persistently bound radiolabel is confined to monoaminergic neurons suggests that it is irreversibly bound to its target site, the amine-storing vesicle. Support for this interpretation comes from studies demonstrating a fast anterograde axonal transport of (/sup 3/H) reserpine in the nigrostriatal tract after intranigral injection of the radiolabel. These findings demonstrate that (/sup 3/H) reserpine of high specific activity is a useful tool in studies designed to map monoaminergic pathways in the brain and to further characterize amine-storing mechanisms.}
doi = {10.1016/0306-4522(79)90177-5}
journal = []
volume = {4:7}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Jan}
}