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Mapping monoaminergic neurons with (/sup 3/H) reserpine by autoradiography

Abstract

The synthesis of (/sup 3/H) reserpine of high specific activity is described. The accumulation of radioactivity in peripheral sympathetically innervated organs and in the brain after intravenous injection of (/sup 3/H) reserpine to rats was measured biochemically and its localization studied by light-microscopic autoradiography. In most of the organs and tissues investigated minute quantities of (/sup 3/H) reserpine persisted up to 10 days after injection. By autoradiography, it was observed that silver grains were unevenly distributed in various brain regions and peripheral organs 18 h and up to 10 days after administration of (/sup 3/H) reserpine. Pretreating (but not post-treating) animals with non-radioactive reserpine prevented by up to 80% the accumulation of radiolabel and abolished to a great extent the autoradiographic localization. The fact that the persistently bound radiolabel is confined to monoaminergic neurons suggests that it is irreversibly bound to its target site, the amine-storing vesicle. Support for this interpretation comes from studies demonstrating a fast anterograde axonal transport of (/sup 3/H) reserpine in the nigrostriatal tract after intranigral injection of the radiolabel. These findings demonstrate that (/sup 3/H) reserpine of high specific activity is a useful tool in studies designed to map monoaminergic pathways in the brain and  More>>
Authors:
Richards, J G; Da Prada, M; Wuersch, J; Lorez, H P; Pieri, L [1] 
  1. Hoffmann-La Roche (F.) and Co., Basel (Switzerland)
Publication Date:
Jan 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-12-591744; EDB-81-076587
Resource Relation:
Journal Name: Neuroscience; (United Kingdom); Journal Volume: 4:7
Subject:
59 BASIC BIOLOGICAL SCIENCES; AMINES; BIOLOGICAL PATHWAYS; NERVE CELLS; MAPPING; RESERPINE; BIOLOGICAL LOCALIZATION; AUTONOMIC NERVOUS SYSTEM; AUTORADIOGRAPHY; BRAIN; INTRAVENOUS INJECTION; LABELLING; RATS; SYNTHESIS; TISSUE DISTRIBUTION; TRACER TECHNIQUES; TRITIUM; ALKALOIDS; ANIMAL CELLS; ANIMALS; ANTIHYPERTENSIVE AGENTS; AUTONOMIC NERVOUS SYSTEM AGENTS; AZOLES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARDIOVASCULAR AGENTS; CENTRAL NERVOUS SYSTEM; DISTRIBUTION; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROGEN ISOTOPES; INDOLES; INJECTION; INTAKE; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRROLES; RADIOISOTOPES; RODENTS; SOMATIC CELLS; SYMPATHOLYTICS; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 551001* - Physiological Systems- Tracer Techniques
OSTI ID:
6409475
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: NRSCD
Submitting Site:
INIS
Size:
Pages: 937-950
Announcement Date:
Mar 01, 1981

Citation Formats

Richards, J G, Da Prada, M, Wuersch, J, Lorez, H P, and Pieri, L. Mapping monoaminergic neurons with (/sup 3/H) reserpine by autoradiography. United Kingdom: N. p., 1979. Web. doi:10.1016/0306-4522(79)90177-5.
Richards, J G, Da Prada, M, Wuersch, J, Lorez, H P, & Pieri, L. Mapping monoaminergic neurons with (/sup 3/H) reserpine by autoradiography. United Kingdom. https://doi.org/10.1016/0306-4522(79)90177-5
Richards, J G, Da Prada, M, Wuersch, J, Lorez, H P, and Pieri, L. 1979. "Mapping monoaminergic neurons with (/sup 3/H) reserpine by autoradiography." United Kingdom. https://doi.org/10.1016/0306-4522(79)90177-5.
@misc{etde_6409475,
title = {Mapping monoaminergic neurons with (/sup 3/H) reserpine by autoradiography}
author = {Richards, J G, Da Prada, M, Wuersch, J, Lorez, H P, and Pieri, L}
abstractNote = {The synthesis of (/sup 3/H) reserpine of high specific activity is described. The accumulation of radioactivity in peripheral sympathetically innervated organs and in the brain after intravenous injection of (/sup 3/H) reserpine to rats was measured biochemically and its localization studied by light-microscopic autoradiography. In most of the organs and tissues investigated minute quantities of (/sup 3/H) reserpine persisted up to 10 days after injection. By autoradiography, it was observed that silver grains were unevenly distributed in various brain regions and peripheral organs 18 h and up to 10 days after administration of (/sup 3/H) reserpine. Pretreating (but not post-treating) animals with non-radioactive reserpine prevented by up to 80% the accumulation of radiolabel and abolished to a great extent the autoradiographic localization. The fact that the persistently bound radiolabel is confined to monoaminergic neurons suggests that it is irreversibly bound to its target site, the amine-storing vesicle. Support for this interpretation comes from studies demonstrating a fast anterograde axonal transport of (/sup 3/H) reserpine in the nigrostriatal tract after intranigral injection of the radiolabel. These findings demonstrate that (/sup 3/H) reserpine of high specific activity is a useful tool in studies designed to map monoaminergic pathways in the brain and to further characterize amine-storing mechanisms.}
doi = {10.1016/0306-4522(79)90177-5}
journal = []
volume = {4:7}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Jan}
}