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DNA probes

Abstract

The creation of DNA probes for detection of specific nucleotide segments differs from ligand detection in that it is a chemical rather than an immunological reaction. Complementary DNA or RNA is used in place of the antibody and is labelled with {sup 32}P. So far, DNA probes have been successfully employed in the diagnosis of inherited disorders, infectious diseases, and for identification of human oncogenes. The latest approach to the diagnosis of communicable and parasitic infections is based on the use of deoxyribonucleic acid (DNA) probes. The genetic information of all cells is encoded by DNA and DNA probe approach to identification of pathogens is unique because the focus of the method is the nucleic acid content of the organism rather than the products that the nucleic acid encodes. Since every properly classified species has some unique nucleotide sequences that distinguish it from every other species, each organism`s genetic composition is in essence a finger print that can be used for its identification. In addition to this specificity, DNA probes offer other advantages in that pathogens may be identified directly in clinical specimens 10 figs, 2 tabs
Authors:
Publication Date:
Dec 31, 1992
Product Type:
Technical Report
Report Number:
NMS-1
Reference Number:
SCA: 550601; 553006; PA: AIX-29:049613; EDB-98:095842; SN: 98002003649
Resource Relation:
Other Information: DN: 10 figs, 2 tabs; PBD: 1992; Related Information: Is Part Of Handbook of nuclear medicine practice in developing countries; PB: 728 p.
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DEVELOPING COUNTRIES; DIAGNOSTIC TECHNIQUES; DIAGNOSTIC USES; DNA; GENETIC ENGINEERING; MEASURING INSTRUMENTS; MOLECULAR BIOLOGY; PATHOGENS; PHOSPHORUS 32; PROBES; PROTEINS; RADIOIMMUNOASSAY; RECOMBINANT DNA
OSTI ID:
640601
Research Organizations:
International Atomic Energy Agency, Vienna (Austria). Nuclear Medicine Section
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ON: DE98635463; TRN: XA9847615049613
Availability:
INIS; OSTI as DE98635463
Submitting Site:
INIS
Size:
pp. 273-291
Announcement Date:

Citation Formats

Castelino, J. DNA probes. IAEA: N. p., 1992. Web.
Castelino, J. DNA probes. IAEA.
Castelino, J. 1992. "DNA probes." IAEA.
@misc{etde_640601,
title = {DNA probes}
author = {Castelino, J}
abstractNote = {The creation of DNA probes for detection of specific nucleotide segments differs from ligand detection in that it is a chemical rather than an immunological reaction. Complementary DNA or RNA is used in place of the antibody and is labelled with {sup 32}P. So far, DNA probes have been successfully employed in the diagnosis of inherited disorders, infectious diseases, and for identification of human oncogenes. The latest approach to the diagnosis of communicable and parasitic infections is based on the use of deoxyribonucleic acid (DNA) probes. The genetic information of all cells is encoded by DNA and DNA probe approach to identification of pathogens is unique because the focus of the method is the nucleic acid content of the organism rather than the products that the nucleic acid encodes. Since every properly classified species has some unique nucleotide sequences that distinguish it from every other species, each organism`s genetic composition is in essence a finger print that can be used for its identification. In addition to this specificity, DNA probes offer other advantages in that pathogens may be identified directly in clinical specimens 10 figs, 2 tabs}
place = {IAEA}
year = {1992}
month = {Dec}
}