You need JavaScript to view this

Improved radioimmunoassay for urinary Tamm-Horsfall glycoprotein. Investigation and resolution of factors affecting its quantification

Journal Article:

Abstract

A rapid specific radioimmunoassay has been used to measure Tamm-Horsfall glycoprotein (TH glycoprotein) in urine, and the method described. The apparent concentration increased with increasing dilution of urine in water, reaching a plateau at 1 in 20. This increase was greater the higher the osmolality and TH glycoprotein concentration and the lower the pH of the original sample. The apparent concentration of TH glycoprotein in neat or diluted urine was not affected by freezing or by storage at 4/sup 0/C or room temperature for at least 2 days. A physiological range for the urinary excretion rate was established as 22-56 mg/24h, (considerably higher than the amount present in serum) based on samples from 29 individuals with normal renal function, as defined by their creatinine clearance. There was no significant correlation between serum concentrations of TH glycoprotein and its urinary excretion rate, nor between urinary excretion rate and creatinine clearance.
Authors:
Dawney, A B.St.J.; Thornley, C; Cattell, W R [1] 
  1. Saint Bartholomew's Hospital, London (UK)
Publication Date:
Sep 15, 1982
Product Type:
Journal Article
Reference Number:
AIX-14-746335; EDB-83-087987
Resource Relation:
Journal Name: Biochem. J.; (United Kingdom); Journal Volume: 206:3
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; GLUCOPROTEINS; RADIOIMMUNOASSAY; BLOOD SERUM; CREATININE; EXCRETION; TEMPERATURE DEPENDENCE; TIME DEPENDENCE; URINE; AZOLES; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY FLUIDS; CARBOHYDRATES; CLEARANCE; HETEROCYCLIC COMPOUNDS; IMIDAZOLES; IMINES; ISOTOPE APPLICATIONS; MATERIALS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PROTEINS; RADIOASSAY; SACCHARIDES; TRACER TECHNIQUES; WASTES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6383695
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BIJOA
Submitting Site:
HEDB
Size:
Pages: 461-465
Announcement Date:

Journal Article:

Citation Formats

Dawney, A B.St.J., Thornley, C, and Cattell, W R. Improved radioimmunoassay for urinary Tamm-Horsfall glycoprotein. Investigation and resolution of factors affecting its quantification. United Kingdom: N. p., 1982. Web.
Dawney, A B.St.J., Thornley, C, & Cattell, W R. Improved radioimmunoassay for urinary Tamm-Horsfall glycoprotein. Investigation and resolution of factors affecting its quantification. United Kingdom.
Dawney, A B.St.J., Thornley, C, and Cattell, W R. 1982. "Improved radioimmunoassay for urinary Tamm-Horsfall glycoprotein. Investigation and resolution of factors affecting its quantification." United Kingdom.
@misc{etde_6383695,
title = {Improved radioimmunoassay for urinary Tamm-Horsfall glycoprotein. Investigation and resolution of factors affecting its quantification}
author = {Dawney, A B.St.J., Thornley, C, and Cattell, W R}
abstractNote = {A rapid specific radioimmunoassay has been used to measure Tamm-Horsfall glycoprotein (TH glycoprotein) in urine, and the method described. The apparent concentration increased with increasing dilution of urine in water, reaching a plateau at 1 in 20. This increase was greater the higher the osmolality and TH glycoprotein concentration and the lower the pH of the original sample. The apparent concentration of TH glycoprotein in neat or diluted urine was not affected by freezing or by storage at 4/sup 0/C or room temperature for at least 2 days. A physiological range for the urinary excretion rate was established as 22-56 mg/24h, (considerably higher than the amount present in serum) based on samples from 29 individuals with normal renal function, as defined by their creatinine clearance. There was no significant correlation between serum concentrations of TH glycoprotein and its urinary excretion rate, nor between urinary excretion rate and creatinine clearance.}
journal = {Biochem. J.; (United Kingdom)}
volume = {206:3}
journal type = {AC}
place = {United Kingdom}
year = {1982}
month = {Sep}
}