Abstract
Two basic analytical procedures are described for the detection of photoproducts in UV-irradiated DNA. In the former, the selective release of thymine dimers of the cyclobutane type (TT) from the UV-irradiated DNA during excision repair can be measured by chromatographic analysis of radioactive DNA hydrolysis products. The technique allows studying TT irrespective of other products. It is only reliable for UV doses higher than 5 Jm/sup -2/. In the latter, a Micrococcus luteus extract containing specific enzymes, ie., endonucleases, for the repair of UV-induced damage of DNA is used for the enzyme determination of pyrimidine dimers. The endonucleotide analysis of DNA damage can be applied both in vitro and in vivo. In the in-vitro detection, the efficacy of photoproduct determination attains almost 100% while in the in-vivo detection it ranges between 30% and 70% in dependence on the method used. 31 references are given.
Kleibl, K;
Brozmanova, J
[1]
- Slovenska Akademia Vied, Bratislava (Czechoslovakia). Ustav Experimentalnej Onkologie
Citation Formats
Kleibl, K, and Brozmanova, J.
Enzymatic determination of photoproducts in DNA molecules damaged by UV radiation.
Serbia and Montenegro: N. p.,
1981.
Web.
Kleibl, K, & Brozmanova, J.
Enzymatic determination of photoproducts in DNA molecules damaged by UV radiation.
Serbia and Montenegro.
Kleibl, K, and Brozmanova, J.
1981.
"Enzymatic determination of photoproducts in DNA molecules damaged by UV radiation."
Serbia and Montenegro.
@misc{etde_6376427,
title = {Enzymatic determination of photoproducts in DNA molecules damaged by UV radiation}
author = {Kleibl, K, and Brozmanova, J}
abstractNote = {Two basic analytical procedures are described for the detection of photoproducts in UV-irradiated DNA. In the former, the selective release of thymine dimers of the cyclobutane type (TT) from the UV-irradiated DNA during excision repair can be measured by chromatographic analysis of radioactive DNA hydrolysis products. The technique allows studying TT irrespective of other products. It is only reliable for UV doses higher than 5 Jm/sup -2/. In the latter, a Micrococcus luteus extract containing specific enzymes, ie., endonucleases, for the repair of UV-induced damage of DNA is used for the enzyme determination of pyrimidine dimers. The endonucleotide analysis of DNA damage can be applied both in vitro and in vivo. In the in-vitro detection, the efficacy of photoproduct determination attains almost 100% while in the in-vivo detection it ranges between 30% and 70% in dependence on the method used. 31 references are given.}
journal = []
volume = {36:6}
journal type = {AC}
place = {Serbia and Montenegro}
year = {1981}
month = {Jan}
}
title = {Enzymatic determination of photoproducts in DNA molecules damaged by UV radiation}
author = {Kleibl, K, and Brozmanova, J}
abstractNote = {Two basic analytical procedures are described for the detection of photoproducts in UV-irradiated DNA. In the former, the selective release of thymine dimers of the cyclobutane type (TT) from the UV-irradiated DNA during excision repair can be measured by chromatographic analysis of radioactive DNA hydrolysis products. The technique allows studying TT irrespective of other products. It is only reliable for UV doses higher than 5 Jm/sup -2/. In the latter, a Micrococcus luteus extract containing specific enzymes, ie., endonucleases, for the repair of UV-induced damage of DNA is used for the enzyme determination of pyrimidine dimers. The endonucleotide analysis of DNA damage can be applied both in vitro and in vivo. In the in-vitro detection, the efficacy of photoproduct determination attains almost 100% while in the in-vivo detection it ranges between 30% and 70% in dependence on the method used. 31 references are given.}
journal = []
volume = {36:6}
journal type = {AC}
place = {Serbia and Montenegro}
year = {1981}
month = {Jan}
}