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Enzymatic determination of photoproducts in DNA molecules damaged by UV radiation

Journal Article:

Abstract

Two basic analytical procedures are described for the detection of photoproducts in UV-irradiated DNA. In the former, the selective release of thymine dimers of the cyclobutane type (TT) from the UV-irradiated DNA during excision repair can be measured by chromatographic analysis of radioactive DNA hydrolysis products. The technique allows studying TT irrespective of other products. It is only reliable for UV doses higher than 5 Jm/sup -2/. In the latter, a Micrococcus luteus extract containing specific enzymes, ie., endonucleases, for the repair of UV-induced damage of DNA is used for the enzyme determination of pyrimidine dimers. The endonucleotide analysis of DNA damage can be applied both in vitro and in vivo. In the in-vitro detection, the efficacy of photoproduct determination attains almost 100% while in the in-vivo detection it ranges between 30% and 70% in dependence on the method used. 31 references are given.
Authors:
Kleibl, K; Brozmanova, J [1] 
  1. Slovenska Akademia Vied, Bratislava (Czechoslovakia). Ustav Experimentalnej Onkologie
Publication Date:
Jan 01, 1981
Product Type:
Journal Article
Reference Number:
AIX-14-731673; EDB-83-069731
Resource Relation:
Journal Name: Biologia (Bratislava); (Czechoslovakia); Journal Volume: 36:6
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; CHEMICAL RADIATION EFFECTS; DIMERS; ESCHERICHIA COLI; IN VITRO; IN VIVO; MICROCOCCUS LUTEUS; NUCLEASES; PHOTOCHEMICAL REACTIONS; ULTRAVIOLET RADIATION; BACTERIA; CHEMICAL REACTIONS; CHEMISTRY; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERASES; HYDROLASES; MICROCOCCUS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; 560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
OSTI ID:
6376427
Country of Origin:
Serbia and Montenegro
Language:
Slovak
Other Identifying Numbers:
Journal ID: CODEN: BLOAA
Submitting Site:
HEDB
Size:
Pages: 456-466
Announcement Date:

Journal Article:

Citation Formats

Kleibl, K, and Brozmanova, J. Enzymatic determination of photoproducts in DNA molecules damaged by UV radiation. Serbia and Montenegro: N. p., 1981. Web.
Kleibl, K, & Brozmanova, J. Enzymatic determination of photoproducts in DNA molecules damaged by UV radiation. Serbia and Montenegro.
Kleibl, K, and Brozmanova, J. 1981. "Enzymatic determination of photoproducts in DNA molecules damaged by UV radiation." Serbia and Montenegro.
@misc{etde_6376427,
title = {Enzymatic determination of photoproducts in DNA molecules damaged by UV radiation}
author = {Kleibl, K, and Brozmanova, J}
abstractNote = {Two basic analytical procedures are described for the detection of photoproducts in UV-irradiated DNA. In the former, the selective release of thymine dimers of the cyclobutane type (TT) from the UV-irradiated DNA during excision repair can be measured by chromatographic analysis of radioactive DNA hydrolysis products. The technique allows studying TT irrespective of other products. It is only reliable for UV doses higher than 5 Jm/sup -2/. In the latter, a Micrococcus luteus extract containing specific enzymes, ie., endonucleases, for the repair of UV-induced damage of DNA is used for the enzyme determination of pyrimidine dimers. The endonucleotide analysis of DNA damage can be applied both in vitro and in vivo. In the in-vitro detection, the efficacy of photoproduct determination attains almost 100% while in the in-vivo detection it ranges between 30% and 70% in dependence on the method used. 31 references are given.}
journal = {Biologia (Bratislava); (Czechoslovakia)}
volume = {36:6}
journal type = {AC}
place = {Serbia and Montenegro}
year = {1981}
month = {Jan}
}