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Formation of ethanol from lactose by Zymomonas mobilis

Journal Article:

Abstract

The lactose transposon Tn951 on the IncP plasmid RP1, was introduced into a strain of Zymomonas mobilis. The lac Z gene was expressed and US -galactosidase was produced. The synthesis of US -galactosidase was induced by either isopropyl-US -D-thiogalactopyranoside or lactose, and was not sensitive to catabolite repression. Cells of Zymomonas mobilis containing Tn951 were unable to form colonies on lactose plates, but in liquid medium produced ethanol from lactose as the sole carbon source. The original strain of Zymomonas mobilis without Tn951 produced little or no US -galactosidase and was unable to produce ethanol from lactose in liquid medium.
Publication Date:
Jan 01, 1984
Product Type:
Journal Article
Reference Number:
DE-85-014705; EDB-86-031965
Resource Relation:
Journal Name: J. Biotech.; (Netherlands); Journal Volume: 1:3-4
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; ETHANOL; BIOSYNTHESIS; GALACTOSIDASE; LACTOSE; FERMENTATION; ZYMOMONAS MOBILIS; GENETIC ENGINEERING; CARBON; CELL CULTURES; CULTURE MEDIA; GENES; PRODUCTIVITY; ALCOHOLS; BACTERIA; BIOCONVERSION; CARBOHYDRATES; DISACCHARIDES; ELEMENTS; ENZYMES; GLYCOSYL HYDROLASES; HYDROLASES; HYDROXY COMPOUNDS; MICROORGANISMS; NONMETALS; O-GLYCOSYL HYDROLASES; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; SACCHARIDES; SYNTHESIS; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 550700 - Microbiology; 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
OSTI ID:
6357392
Research Organizations:
Univ. of New South Wales, Sydney, Australia
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JBITD
Submitting Site:
HEDB
Size:
Pages: 219-228
Announcement Date:

Journal Article:

Citation Formats

Goodman, A E, Strzelecki, A T, and Rogers, P L. Formation of ethanol from lactose by Zymomonas mobilis. Netherlands: N. p., 1984. Web.
Goodman, A E, Strzelecki, A T, & Rogers, P L. Formation of ethanol from lactose by Zymomonas mobilis. Netherlands.
Goodman, A E, Strzelecki, A T, and Rogers, P L. 1984. "Formation of ethanol from lactose by Zymomonas mobilis." Netherlands.
@misc{etde_6357392,
title = {Formation of ethanol from lactose by Zymomonas mobilis}
author = {Goodman, A E, Strzelecki, A T, and Rogers, P L}
abstractNote = {The lactose transposon Tn951 on the IncP plasmid RP1, was introduced into a strain of Zymomonas mobilis. The lac Z gene was expressed and US -galactosidase was produced. The synthesis of US -galactosidase was induced by either isopropyl-US -D-thiogalactopyranoside or lactose, and was not sensitive to catabolite repression. Cells of Zymomonas mobilis containing Tn951 were unable to form colonies on lactose plates, but in liquid medium produced ethanol from lactose as the sole carbon source. The original strain of Zymomonas mobilis without Tn951 produced little or no US -galactosidase and was unable to produce ethanol from lactose in liquid medium.}
journal = {J. Biotech.; (Netherlands)}
volume = {1:3-4}
journal type = {AC}
place = {Netherlands}
year = {1984}
month = {Jan}
}