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Radiometric microassay for glutamic acid decarboxylase

Abstract

A simple method for purifying L-(/sup 3/H) glutamic acid and incubation conditions suitable for estimating L-glutamic acid decarboxylase activity are described. Routine and recycled cation-exchange procedure for separating ..gamma..-aminobutyric acid from L-glutamate are outlined and compared. Recycling increases the sensitivity of the cation-exchange method by 6-7 fold. L-Glutamate decarboxylase activity can be measured reliably in samples of embryonic neural tissue having wet-weights of approximately 1 ..mu..g. The cation-exchange method is compared with the anion-exchange and CO/sub 2/-trapping methods. L-Glutamate decarboxylase activity has been detected in the lumbar spinal cord of the chick embryo at Day 21/4 (stage 14) using the cation-exchange method. This is 5-6 days earlier than L-glutamate decarboxylase activity has been detected in embryonic neural tissue by previous investigators. L-Glutamate decarboxylase is present in the lumbar spinal cord at least as early as the birth of the first lumbar spinal cord neurons and at least 1-2 days before the initiation of synaptogenesis.
Authors:
Maderdrut, J L; [1]  North Carolina Univ., Chapel Hill (USA). School of Medicine)
  1. North Carolina Dept. of Mental Health, Raleigh (USA)
Publication Date:
Jan 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-12-591745; EDB-81-076209
Resource Relation:
Journal Name: Neuroscience; (United Kingdom); Journal Volume: 4:7
Subject:
59 BASIC BIOLOGICAL SCIENCES; DECARBOXYLASES; RADIOASSAY; AMINOBUTYRIC ACID; CATALYSIS; CATIONS; CHICKENS; COMPARATIVE EVALUATIONS; EMBRYOS; GLUTAMIC ACID; INCUBATION; ION EXCHANGE CHROMATOGRAPHY; LABELLED COMPOUNDS; METABOLISM; NERVE TISSUE; PURIFICATION; SENSITIVITY; SPECIFICITY; SPINAL CORD; TRITIUM; AMINO ACIDS; ANIMAL TISSUES; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIRDS; BODY; CARBON-CARBON LYASES; CARBOXY-LYASES; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; CHARGED PARTICLES; CHROMATOGRAPHY; DRUGS; ENZYMES; FOWL; HYDROGEN ISOTOPES; IONS; ISOTOPES; LIGHT NUCLEI; LYASES; NERVOUS SYSTEM; NEUROREGULATORS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; RADIOISOTOPES; SEPARATION PROCESSES; TISSUES; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 550201* - Biochemistry- Tracer Techniques
OSTI ID:
6357025
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: NRSCD
Submitting Site:
INIS
Size:
Pages: 995-1005
Announcement Date:

Citation Formats

Maderdrut, J L, and North Carolina Univ., Chapel Hill (USA). School of Medicine). Radiometric microassay for glutamic acid decarboxylase. United Kingdom: N. p., 1979. Web. doi:10.1016/0306-4522(79)90182-9.
Maderdrut, J L, & North Carolina Univ., Chapel Hill (USA). School of Medicine). Radiometric microassay for glutamic acid decarboxylase. United Kingdom. doi:10.1016/0306-4522(79)90182-9.
Maderdrut, J L, and North Carolina Univ., Chapel Hill (USA). School of Medicine). 1979. "Radiometric microassay for glutamic acid decarboxylase." United Kingdom. doi:10.1016/0306-4522(79)90182-9. https://www.osti.gov/servlets/purl/10.1016/0306-4522(79)90182-9.
@misc{etde_6357025,
title = {Radiometric microassay for glutamic acid decarboxylase}
author = {Maderdrut, J L, and North Carolina Univ., Chapel Hill (USA). School of Medicine)}
abstractNote = {A simple method for purifying L-(/sup 3/H) glutamic acid and incubation conditions suitable for estimating L-glutamic acid decarboxylase activity are described. Routine and recycled cation-exchange procedure for separating ..gamma..-aminobutyric acid from L-glutamate are outlined and compared. Recycling increases the sensitivity of the cation-exchange method by 6-7 fold. L-Glutamate decarboxylase activity can be measured reliably in samples of embryonic neural tissue having wet-weights of approximately 1 ..mu..g. The cation-exchange method is compared with the anion-exchange and CO/sub 2/-trapping methods. L-Glutamate decarboxylase activity has been detected in the lumbar spinal cord of the chick embryo at Day 21/4 (stage 14) using the cation-exchange method. This is 5-6 days earlier than L-glutamate decarboxylase activity has been detected in embryonic neural tissue by previous investigators. L-Glutamate decarboxylase is present in the lumbar spinal cord at least as early as the birth of the first lumbar spinal cord neurons and at least 1-2 days before the initiation of synaptogenesis.}
doi = {10.1016/0306-4522(79)90182-9}
journal = {Neuroscience; (United Kingdom)}
volume = {4:7}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Jan}
}