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Calcitonin-like immunoreactivity and calcitonin gene expression in the placenta and in the mammary gland of the rat

Journal Article:

Abstract

Recently, the presence of monomeric CT in plasma and milk was reported by others in a lactating woman surgically thyroidectomized. Similarly, the placenta was thought to be a possible source of CT. Since such findings were based exclusively on immunological arguments, we have investigated the CT gene expression in these rat tissues. CT mRNAs were detected by dot-blot hybridization of total RNAs extracted from rat tissues with a /sup 32/P-labelled human CT cDNa probe. Subcellular fractions of each tissue were screened for CT-like immunoreactivity using two different antibodies. With one antibody, extracts of the mammary gland and placenta both produced full displacement of labelled human CT from the antiserum and serial dilutions of the extracts gave displacement curves parallel to that of synthetic human CT, which suggests immunological similarity. However, dilution curves were not parallel for the second antibody, and for both antisera, CT-like immunoreactivity was found in all subsellular fractions from nuclei to cytosols. Immunoprecipitation of translation products from poly (A)/sup +/RNAs of placenta showed two major bands around 30 kD. Under stringent conditions, the weak hybridization of placental RNAs seen by dot-blot under less stringent conditions disappeared. Northern analyses of total RNAs from the placenta failed to detect  More>>
Publication Date:
Jan 01, 1988
Product Type:
Journal Article
Reference Number:
AIX-20-022217; EDB-89-042755
Resource Relation:
Journal Name: Acta Endocrinol.; (Denmark); Journal Volume: 119
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CALCITONIN; GENES; PLACENTA; IMMUNITY; MAMMARY GLANDS; PHOSPHORUS 32; RATS; RNA; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; FETAL MEMBRANES; GLANDS; HORMONES; ISOTOPES; LIGHT NUCLEI; MAMMALS; MEMBRANES; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PEPTIDES; PHOSPHORUS ISOTOPES; POLYPEPTIDES; PROTEINS; RADIOISOTOPES; RODENTS; VERTEBRATES; 560162* - Radionuclide Effects, Kinetics, & Toxicology- Animals, Plants, Microorganisms, & Cells
OSTI ID:
6350315
Research Organizations:
Endocrinologie Moleculaire et Metabolique, UA CNRS 1123, Universite Pierre et Marie Curie (France); Unite 113 de l'INSERM, C.H.U. Saint-Antoine, Paris (France)
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ACENA
Submitting Site:
INIS
Size:
Pages: 119
Announcement Date:

Journal Article:

Citation Formats

Jousset, V, Legendre, B, Besnard, P, Segond, N, and Jullienne, A. Calcitonin-like immunoreactivity and calcitonin gene expression in the placenta and in the mammary gland of the rat. Denmark: N. p., 1988. Web.
Jousset, V, Legendre, B, Besnard, P, Segond, N, & Jullienne, A. Calcitonin-like immunoreactivity and calcitonin gene expression in the placenta and in the mammary gland of the rat. Denmark.
Jousset, V, Legendre, B, Besnard, P, Segond, N, and Jullienne, A. 1988. "Calcitonin-like immunoreactivity and calcitonin gene expression in the placenta and in the mammary gland of the rat." Denmark.
@misc{etde_6350315,
title = {Calcitonin-like immunoreactivity and calcitonin gene expression in the placenta and in the mammary gland of the rat}
author = {Jousset, V, Legendre, B, Besnard, P, Segond, N, and Jullienne, A}
abstractNote = {Recently, the presence of monomeric CT in plasma and milk was reported by others in a lactating woman surgically thyroidectomized. Similarly, the placenta was thought to be a possible source of CT. Since such findings were based exclusively on immunological arguments, we have investigated the CT gene expression in these rat tissues. CT mRNAs were detected by dot-blot hybridization of total RNAs extracted from rat tissues with a /sup 32/P-labelled human CT cDNa probe. Subcellular fractions of each tissue were screened for CT-like immunoreactivity using two different antibodies. With one antibody, extracts of the mammary gland and placenta both produced full displacement of labelled human CT from the antiserum and serial dilutions of the extracts gave displacement curves parallel to that of synthetic human CT, which suggests immunological similarity. However, dilution curves were not parallel for the second antibody, and for both antisera, CT-like immunoreactivity was found in all subsellular fractions from nuclei to cytosols. Immunoprecipitation of translation products from poly (A)/sup +/RNAs of placenta showed two major bands around 30 kD. Under stringent conditions, the weak hybridization of placental RNAs seen by dot-blot under less stringent conditions disappeared. Northern analyses of total RNAs from the placenta failed to detect mRNA of 1 k base size like in thyroid glands, but hybridization under weak stringent conditions occurred with larger mRNAs (around 4.4 and 2.4 k bases). Immunoprecipitation of translation products from mRNAs of rat mammary glands showed three major bands around 46, 30 and 20 kD. Our results suggest that the CT gene is not expressed in the rat placenta and in rat mammary gland, since CT mRNAs were not detected in either tissues. (EB).}
journal = {Acta Endocrinol.; (Denmark)}
volume = {119}
journal type = {AC}
place = {Denmark}
year = {1988}
month = {Jan}
}