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Occurrence and elimination of sites sensitive to UV-endonuclease in UV-irradiated E. coli cells

Journal Article:

Abstract

The occurrence and elimination of sites sensitive to the endonucleolytic action of crude extract from M. luteus (Es sites) were studied in both the parental and daughter DNA of E. coli B/r Hcr/sup +/ irradiated either with lethal fluence only (LF) or with inducing and lethal fluence (IF+LF); after the lethal fluence protein synthesis could either take place or it was inhibited by chlorampehnicol (CAP). The data obtained showed that in the wild type UV-irradiated cells Es sites could be eliminated from their DNA molecules either through pyrimidine dimer excision or through the modification of dimers on replication. It appears that DNA repair takes place most efficiently in cells irradiated with IF+LF and postincubated with CAP; in these conditions cells are supplied with inducible proteins, and enough time for DNA repair is provided before the division of irradiated cells is resumed.
Authors:
Kleibl, K; Sedliakova, M [1] 
  1. Slovenska Akademia Vied, Bratislava (Czechoslovakia). Vyskumny Ustav Onkologicky
Publication Date:
Jan 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-12-595462; EDB-81-106319
Resource Relation:
Journal Name: Biologia (Bratislava); (Czechoslovakia); Journal Volume: 34:9
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; BIOLOGICAL REPAIR; NUCLEASES; BIOCHEMICAL REACTION KINETICS; CHLORAMPHENICOL; DOSE-RESPONSE RELATIONSHIPS; ESCHERICHIA COLI; LETHAL IRRADIATION; MICROCOCCUS LUTEUS; NEAR ULTRAVIOLET RADIATION; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; BACTERIA; BIOLOGICAL RECOVERY; DRUGS; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERASES; HYDROLASES; IRRADIATION; KINETICS; MICROCOCCUS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; RADIATIONS; REACTION KINETICS; RECOVERY; REPAIR; ULTRAVIOLET RADIATION; 550700* - Microbiology; 560400 - Other Environmental Pollutant Effects
OSTI ID:
6341467
Country of Origin:
Serbia and Montenegro
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BLOAA
Submitting Site:
INIS
Size:
Pages: 681-690
Announcement Date:

Journal Article:

Citation Formats

Kleibl, K, and Sedliakova, M. Occurrence and elimination of sites sensitive to UV-endonuclease in UV-irradiated E. coli cells. Serbia and Montenegro: N. p., 1979. Web.
Kleibl, K, & Sedliakova, M. Occurrence and elimination of sites sensitive to UV-endonuclease in UV-irradiated E. coli cells. Serbia and Montenegro.
Kleibl, K, and Sedliakova, M. 1979. "Occurrence and elimination of sites sensitive to UV-endonuclease in UV-irradiated E. coli cells." Serbia and Montenegro.
@misc{etde_6341467,
title = {Occurrence and elimination of sites sensitive to UV-endonuclease in UV-irradiated E. coli cells}
author = {Kleibl, K, and Sedliakova, M}
abstractNote = {The occurrence and elimination of sites sensitive to the endonucleolytic action of crude extract from M. luteus (Es sites) were studied in both the parental and daughter DNA of E. coli B/r Hcr/sup +/ irradiated either with lethal fluence only (LF) or with inducing and lethal fluence (IF+LF); after the lethal fluence protein synthesis could either take place or it was inhibited by chlorampehnicol (CAP). The data obtained showed that in the wild type UV-irradiated cells Es sites could be eliminated from their DNA molecules either through pyrimidine dimer excision or through the modification of dimers on replication. It appears that DNA repair takes place most efficiently in cells irradiated with IF+LF and postincubated with CAP; in these conditions cells are supplied with inducible proteins, and enough time for DNA repair is provided before the division of irradiated cells is resumed.}
journal = {Biologia (Bratislava); (Czechoslovakia)}
volume = {34:9}
journal type = {AC}
place = {Serbia and Montenegro}
year = {1979}
month = {Jan}
}