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Patch size and base composition of ultraviolet light-induced repair synthesis in toluenized Escherichia coli

Abstract

Small patch repair in ultraviolet-irradiated Escherichia coli was saturated at deoxynucleoside triphosphate concentrations (approximately 2..mu..M of each dNTP) that are severly limiting for DNA replication. The low requirement of the repair process for dNTPs permitted direct demonstration of u.v.-induced DNA synthesis by incorporation of labelled dNTP and determination of its extent, base composition and patch size. It is concluded that DNA polymerase 1 is involved in small patch repair and that an average of 13 to 16 nucleotides are re-inserted per pyrimidine dimer excised. The average base composition of the repaired stretches adjacent to the dimers is similar to that of total E.coli DNA. An assay utilizing endogenous u.v.-specific endonuclease to determine dimer excision is described.
Authors:
Ben-Ishai, R; Sharon, R [1] 
  1. Technion-Israel Inst. of Tech., Haifa
Publication Date:
Apr 15, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-420841; EDB-79-052049
Resource Relation:
Journal Name: J. Mol. Biol.; (United Kingdom); Journal Volume: 120:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; DNA; BIOLOGICAL REPAIR; NEAR ULTRAVIOLET RADIATION; BIOLOGICAL EFFECTS; ATP; CHEMICAL COMPOSITION; CYTOSINE; DIMERS; ESCHERICHIA COLI; GUANOSINE; LABELLED COMPOUNDS; LABELLING; MOLECULAR STRUCTURE; NAD; PHOSPHORIC ACID ESTERS; PHOSPHORUS 32; POLYMERASES; THYMIDINE; THYMINE; TRITIUM COMPOUNDS; AMINES; AZINES; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL RECOVERY; COENZYMES; DAYS LIVING RADIOISOTOPES; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; PHOSPHORUS ISOTOPES; PURINES; PYRIMIDINES; RADIATIONS; RADIOISOTOPES; RECOVERY; REPAIR; RIBOSIDES; ULTRAVIOLET RADIATION; URACILS; 560400* - Other Environmental Pollutant Effects; 550700 - Microbiology
OSTI ID:
6337961
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JMOBA
Submitting Site:
INIS
Size:
Pages: 423-432
Announcement Date:

Citation Formats

Ben-Ishai, R, and Sharon, R. Patch size and base composition of ultraviolet light-induced repair synthesis in toluenized Escherichia coli. United Kingdom: N. p., 1978. Web. doi:10.1016/0022-2836(78)90428-X.
Ben-Ishai, R, & Sharon, R. Patch size and base composition of ultraviolet light-induced repair synthesis in toluenized Escherichia coli. United Kingdom. doi:10.1016/0022-2836(78)90428-X.
Ben-Ishai, R, and Sharon, R. 1978. "Patch size and base composition of ultraviolet light-induced repair synthesis in toluenized Escherichia coli." United Kingdom. doi:10.1016/0022-2836(78)90428-X. https://www.osti.gov/servlets/purl/10.1016/0022-2836(78)90428-X.
@misc{etde_6337961,
title = {Patch size and base composition of ultraviolet light-induced repair synthesis in toluenized Escherichia coli}
author = {Ben-Ishai, R, and Sharon, R}
abstractNote = {Small patch repair in ultraviolet-irradiated Escherichia coli was saturated at deoxynucleoside triphosphate concentrations (approximately 2..mu..M of each dNTP) that are severly limiting for DNA replication. The low requirement of the repair process for dNTPs permitted direct demonstration of u.v.-induced DNA synthesis by incorporation of labelled dNTP and determination of its extent, base composition and patch size. It is concluded that DNA polymerase 1 is involved in small patch repair and that an average of 13 to 16 nucleotides are re-inserted per pyrimidine dimer excised. The average base composition of the repaired stretches adjacent to the dimers is similar to that of total E.coli DNA. An assay utilizing endogenous u.v.-specific endonuclease to determine dimer excision is described.}
doi = {10.1016/0022-2836(78)90428-X}
journal = {J. Mol. Biol.; (United Kingdom)}
volume = {120:3}
journal type = {AC}
place = {United Kingdom}
year = {1978}
month = {Apr}
}