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The effects of low dose ionizing radiation on the development of rat cerebral cortex, (2); In vitro study with regards to neuronal migration

Journal Article:

Abstract

In order to study the molecular mechanisms of neuronal migration on developing rat cerebral cortex, we need a tissue culture system in which neuronal migration can be observed. We prepared a tissue culture system of embryonic rat cerebral cortex starting on embryonic day 16 and cultivating it for 48 hours. The autoradiographic study in this system revealed not only the migration of [sup 3]H-thymidine labeled neurons but also neuronal migration delays from low doses of ionizing radiation of more than 10 cGy. In addition, on immunohistochemical study, cell-cell adhesion molecule N-CAM staining was remarkably decreased in the matrix cell layer. In the tissue culture system where monoclonal anti-N-CAM antibodies were added, neuronal migration delay comparable to that of 20 cGy radiation was found. In conclusion, it was speculated that neuronal migration delay might be caused by disturbed N-CAM synthesis in matrix cells after low dose ionizing radiation. (author).
Authors:
Matsushita, Koji [1] 
  1. Kyoto Prefectural Univ. of Medicine (Japan)
Publication Date:
Mar 01, 1993
Product Type:
Journal Article
Reference Number:
JPN-93-006316; EDB-93-100162
Resource Relation:
Journal Name: Nippon Shonika Gakkai Zasshi; (Japan); Journal Volume: 97:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CEREBRAL CORTEX; LOW DOSE IRRADIATION; NERVE CELLS; BIOLOGICAL RADIATION EFFECTS; AUTORADIOGRAPHY; CULTURE MEDIA; EMBRYOS; MONOCLONAL ANTIBODIES; RADIATION DOSES; RATS; STAINS; ANIMAL CELLS; ANIMALS; ANTIBODIES; BIOLOGICAL EFFECTS; BODY; BRAIN; CENTRAL NERVOUS SYSTEM; CEREBRUM; DOSES; IRRADIATION; MAMMALS; NERVOUS SYSTEM; ORGANS; RADIATION EFFECTS; RODENTS; SOMATIC CELLS; VERTEBRATES; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture
OSTI ID:
6333498
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: ISSN 0001-6543; CODEN: NIPOAC
Submitting Site:
JPN
Size:
Pages: 669-675
Announcement Date:

Journal Article:

Citation Formats

Matsushita, Koji. The effects of low dose ionizing radiation on the development of rat cerebral cortex, (2); In vitro study with regards to neuronal migration. Japan: N. p., 1993. Web.
Matsushita, Koji. The effects of low dose ionizing radiation on the development of rat cerebral cortex, (2); In vitro study with regards to neuronal migration. Japan.
Matsushita, Koji. 1993. "The effects of low dose ionizing radiation on the development of rat cerebral cortex, (2); In vitro study with regards to neuronal migration." Japan.
@misc{etde_6333498,
title = {The effects of low dose ionizing radiation on the development of rat cerebral cortex, (2); In vitro study with regards to neuronal migration}
author = {Matsushita, Koji}
abstractNote = {In order to study the molecular mechanisms of neuronal migration on developing rat cerebral cortex, we need a tissue culture system in which neuronal migration can be observed. We prepared a tissue culture system of embryonic rat cerebral cortex starting on embryonic day 16 and cultivating it for 48 hours. The autoradiographic study in this system revealed not only the migration of [sup 3]H-thymidine labeled neurons but also neuronal migration delays from low doses of ionizing radiation of more than 10 cGy. In addition, on immunohistochemical study, cell-cell adhesion molecule N-CAM staining was remarkably decreased in the matrix cell layer. In the tissue culture system where monoclonal anti-N-CAM antibodies were added, neuronal migration delay comparable to that of 20 cGy radiation was found. In conclusion, it was speculated that neuronal migration delay might be caused by disturbed N-CAM synthesis in matrix cells after low dose ionizing radiation. (author).}
journal = {Nippon Shonika Gakkai Zasshi; (Japan)}
volume = {97:3}
journal type = {AC}
place = {Japan}
year = {1993}
month = {Mar}
}