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Standardised multicentre procedure for plasma gonadotrophin radioimmunoassay

Journal Article:

Abstract

A radioimmunoassay method for the assay of luteinising hormone (LH) and follicle-stimulating hormone (FSH) in serum/plasma has been designed for use in laboratories of varying expertise in the United Kingdom. The major sources of experimental error leading to poor within-laboratory performance and between-laboratory comparability were identified: quality of tracer, use of calibration standards, and separation procedure. A simple rugged kit was designed which was extensively tested first in our laboratory and then in a small multi-centre field trial before being made available. It is now used routinely by 26 health service and research laboratories. The working range of the assays is 1-50 IU/l (LH) and 0.3-16 IU/l (FSH). The between-batch reproducibility was 5-11% (CV) over the dose range 4.8-18 IU/l (LH) and 1.6-15 IU/l (FSH).
Authors:
Ferguson, K M; Hayes, M; Jeffcoate, S L [1] 
  1. Chelsea Hospital for Women, London (UK)
Publication Date:
Sep 01, 1982
Product Type:
Journal Article
Reference Number:
AIX-14-746022; EDB-83-087988
Resource Relation:
Journal Name: Ann. Clin. Biochem.; (United Kingdom); Journal Volume: 19
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; FSH; RADIOIMMUNOASSAY; LH; BLOOD PLASMA; BLOOD SERUM; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; GONADOTROPINS; HORMONES; ISOTOPE APPLICATIONS; MATERIALS; PEPTIDE HORMONES; PITUITARY HORMONES; RADIOASSAY; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
6302816
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ACBOB
Submitting Site:
HEDB
Size:
Pages: 358-361
Announcement Date:
Mar 01, 1983

Journal Article:

Citation Formats

Ferguson, K M, Hayes, M, and Jeffcoate, S L. Standardised multicentre procedure for plasma gonadotrophin radioimmunoassay. United Kingdom: N. p., 1982. Web.
Ferguson, K M, Hayes, M, & Jeffcoate, S L. Standardised multicentre procedure for plasma gonadotrophin radioimmunoassay. United Kingdom.
Ferguson, K M, Hayes, M, and Jeffcoate, S L. 1982. "Standardised multicentre procedure for plasma gonadotrophin radioimmunoassay." United Kingdom.
@misc{etde_6302816,
title = {Standardised multicentre procedure for plasma gonadotrophin radioimmunoassay}
author = {Ferguson, K M, Hayes, M, and Jeffcoate, S L}
abstractNote = {A radioimmunoassay method for the assay of luteinising hormone (LH) and follicle-stimulating hormone (FSH) in serum/plasma has been designed for use in laboratories of varying expertise in the United Kingdom. The major sources of experimental error leading to poor within-laboratory performance and between-laboratory comparability were identified: quality of tracer, use of calibration standards, and separation procedure. A simple rugged kit was designed which was extensively tested first in our laboratory and then in a small multi-centre field trial before being made available. It is now used routinely by 26 health service and research laboratories. The working range of the assays is 1-50 IU/l (LH) and 0.3-16 IU/l (FSH). The between-batch reproducibility was 5-11% (CV) over the dose range 4.8-18 IU/l (LH) and 1.6-15 IU/l (FSH).}
journal = {Ann. Clin. Biochem.; (United Kingdom)}
volume = {19}
journal type = {AC}
place = {United Kingdom}
year = {1982}
month = {Sep}
}