Abstract
The authors describe the conjugation of DTPA to 100-500 g of protein in concentrations of 0.6-1.0 mg mL utilizing the mixed anhydride method. Free DTPA is removed by minicolumn gel filtration and centrifugation with minimal protein dilution. Radiolabelling process can be monitored by instant thin layer chromatography. Any radiochemical impurity detected can be eliminated either by additional minicolumn filtration of further chelation with more conjugated protein. In citrate buffer at pH 6 with minicolumn gel chromatography the authors prepared In-DTPA-D3 (3.0 Ci g) monoclonal antibody and used it to image hepatocarcinoma in guinea pigs. 13 references, 5 figures, 2 tables.
Citation Formats
Zoghbi, S S, Neumann, R D, and Gottschalk, A.
Modified procedure for rapid labelling of low concentrations of bioactive proteins with indium-111.
United Kingdom: N. p.,
1985.
Web.
doi:10.1016/0047-0740(85)90022-1.
Zoghbi, S S, Neumann, R D, & Gottschalk, A.
Modified procedure for rapid labelling of low concentrations of bioactive proteins with indium-111.
United Kingdom.
https://doi.org/10.1016/0047-0740(85)90022-1
Zoghbi, S S, Neumann, R D, and Gottschalk, A.
1985.
"Modified procedure for rapid labelling of low concentrations of bioactive proteins with indium-111."
United Kingdom.
https://doi.org/10.1016/0047-0740(85)90022-1.
@misc{etde_6297734,
title = {Modified procedure for rapid labelling of low concentrations of bioactive proteins with indium-111}
author = {Zoghbi, S S, Neumann, R D, and Gottschalk, A}
abstractNote = {The authors describe the conjugation of DTPA to 100-500 g of protein in concentrations of 0.6-1.0 mg mL utilizing the mixed anhydride method. Free DTPA is removed by minicolumn gel filtration and centrifugation with minimal protein dilution. Radiolabelling process can be monitored by instant thin layer chromatography. Any radiochemical impurity detected can be eliminated either by additional minicolumn filtration of further chelation with more conjugated protein. In citrate buffer at pH 6 with minicolumn gel chromatography the authors prepared In-DTPA-D3 (3.0 Ci g) monoclonal antibody and used it to image hepatocarcinoma in guinea pigs. 13 references, 5 figures, 2 tables.}
doi = {10.1016/0047-0740(85)90022-1}
journal = []
volume = {12:3}
journal type = {AC}
place = {United Kingdom}
year = {1985}
month = {Jan}
}
title = {Modified procedure for rapid labelling of low concentrations of bioactive proteins with indium-111}
author = {Zoghbi, S S, Neumann, R D, and Gottschalk, A}
abstractNote = {The authors describe the conjugation of DTPA to 100-500 g of protein in concentrations of 0.6-1.0 mg mL utilizing the mixed anhydride method. Free DTPA is removed by minicolumn gel filtration and centrifugation with minimal protein dilution. Radiolabelling process can be monitored by instant thin layer chromatography. Any radiochemical impurity detected can be eliminated either by additional minicolumn filtration of further chelation with more conjugated protein. In citrate buffer at pH 6 with minicolumn gel chromatography the authors prepared In-DTPA-D3 (3.0 Ci g) monoclonal antibody and used it to image hepatocarcinoma in guinea pigs. 13 references, 5 figures, 2 tables.}
doi = {10.1016/0047-0740(85)90022-1}
journal = []
volume = {12:3}
journal type = {AC}
place = {United Kingdom}
year = {1985}
month = {Jan}
}