Abstract
Mathematical models of the TCA cycle derived previously for /sup 14/C tracer studies have been extended to /sup 13/C NMR to measure the /sup 13/C fractional enrichment of (2-/sup 13/C)acetyl-CoA entering the cycle and the relative activities of the oxidative versus anaplerotic pathways. The analysis is based upon the steady-state enrichment of /sup 13/C into the glutamate carbons. Hearts perfused with (2-/sup 13/C)acetate show low but significant activity of the anaplerotic pathways. Activation of two different anaplerotic pathways is demonstrated by addition of unlabeled propionate or pyruvate to hearts perfused with (2-/sup 13/C)acetate. In each case, the amount of (2-/sup 13/C)acetate being oxidized and the relative carbon flux through anaplerotic versus oxidative pathways are evaluated. 18 refs.; 2 figs.; 1 table.
Citation Formats
Malloy, C R, Sherry, A D, and Jeffrey, F M.H.
Carbon flux through citric acid cycle pathways in perfused heart by /sup 13/C NMR spectroscopy.
Netherlands: N. p.,
1987.
Web.
doi:10.1016/0014-5793(87)81556-9.
Malloy, C R, Sherry, A D, & Jeffrey, F M.H.
Carbon flux through citric acid cycle pathways in perfused heart by /sup 13/C NMR spectroscopy.
Netherlands.
https://doi.org/10.1016/0014-5793(87)81556-9
Malloy, C R, Sherry, A D, and Jeffrey, F M.H.
1987.
"Carbon flux through citric acid cycle pathways in perfused heart by /sup 13/C NMR spectroscopy."
Netherlands.
https://doi.org/10.1016/0014-5793(87)81556-9.
@misc{etde_6279690,
title = {Carbon flux through citric acid cycle pathways in perfused heart by /sup 13/C NMR spectroscopy}
author = {Malloy, C R, Sherry, A D, and Jeffrey, F M.H.}
abstractNote = {Mathematical models of the TCA cycle derived previously for /sup 14/C tracer studies have been extended to /sup 13/C NMR to measure the /sup 13/C fractional enrichment of (2-/sup 13/C)acetyl-CoA entering the cycle and the relative activities of the oxidative versus anaplerotic pathways. The analysis is based upon the steady-state enrichment of /sup 13/C into the glutamate carbons. Hearts perfused with (2-/sup 13/C)acetate show low but significant activity of the anaplerotic pathways. Activation of two different anaplerotic pathways is demonstrated by addition of unlabeled propionate or pyruvate to hearts perfused with (2-/sup 13/C)acetate. In each case, the amount of (2-/sup 13/C)acetate being oxidized and the relative carbon flux through anaplerotic versus oxidative pathways are evaluated. 18 refs.; 2 figs.; 1 table.}
doi = {10.1016/0014-5793(87)81556-9}
journal = []
volume = {212:1}
journal type = {AC}
place = {Netherlands}
year = {1987}
month = {Feb}
}
title = {Carbon flux through citric acid cycle pathways in perfused heart by /sup 13/C NMR spectroscopy}
author = {Malloy, C R, Sherry, A D, and Jeffrey, F M.H.}
abstractNote = {Mathematical models of the TCA cycle derived previously for /sup 14/C tracer studies have been extended to /sup 13/C NMR to measure the /sup 13/C fractional enrichment of (2-/sup 13/C)acetyl-CoA entering the cycle and the relative activities of the oxidative versus anaplerotic pathways. The analysis is based upon the steady-state enrichment of /sup 13/C into the glutamate carbons. Hearts perfused with (2-/sup 13/C)acetate show low but significant activity of the anaplerotic pathways. Activation of two different anaplerotic pathways is demonstrated by addition of unlabeled propionate or pyruvate to hearts perfused with (2-/sup 13/C)acetate. In each case, the amount of (2-/sup 13/C)acetate being oxidized and the relative carbon flux through anaplerotic versus oxidative pathways are evaluated. 18 refs.; 2 figs.; 1 table.}
doi = {10.1016/0014-5793(87)81556-9}
journal = []
volume = {212:1}
journal type = {AC}
place = {Netherlands}
year = {1987}
month = {Feb}
}