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Effect of UVB irradiation of the blood on cellular volume adherence and phagocytosis in probands and patients with multiple sclerosis

Journal Article:

Abstract

UVB-induced changes of blood cell properties were investigated in 12 multiple sclerosis (MS) patients and in 10 healthy volunteers. The mean cell volume (MCV) was determined by electronic sizing, the granulocyte and lymphocyte adherence was estimated in a capillary assay, and the phagocytic activity of granulocytes was measured in a test system based on the incorporation of opsonized baker's yeast. In MS patients the MCV of red cells and lymphocytes decreased rapidly within 6 UVB treatments. In contrast, the reduction of the granulocyte volume was delayed (between the 6th and 12th UVB). In the control group the mean value of the red cell and lymphocyte MCV remained rather unaffected. There was a slight rise of the granulocyte volume after the 6th UVB. The only significant change of adherence was an increase of granulocyte adherence in MS patients. Untreated patients had a significantly enhanced phagocytic activity in comparison to the control group. 6 UVB treatments induced a singificant reduction of the phagocytic activity in MS patients. However, subsequently the percentage of phagocytizing cells increased again, whereas the particle uptake per cell continued to decrease. In the control group only minor UVB-induced changes of phagocytosis were observed. The in vitro UV irradiation  More>>
Publication Date:
Jan 01, 1988
Product Type:
Journal Article
Reference Number:
AIX-20-029260; EDB-89-057719
Resource Relation:
Journal Name: Folia Haematol. (Leipzig); (German Democratic Republic); Journal Volume: 115:5
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BLOOD; EXTRACORPOREAL IRRADIATION; NEAR ULTRAVIOLET RADIATION; PATIENTS; NERVOUS SYSTEM DISEASES; ADHESION; BIOASSAY; ERYTHROCYTES; LEUKOCYTES; LYMPHOCYTES; MAN; PHAGOCYTOSIS; VOLUME; ANIMAL CELLS; ANIMALS; BIOLOGICAL MATERIALS; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; ELECTROMAGNETIC RADIATION; EXTERNAL IRRADIATION; IRRADIATION; MAMMALS; MATERIALS; PRIMATES; RADIATIONS; SOMATIC CELLS; ULTRAVIOLET RADIATION; VERTEBRATES; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture
OSTI ID:
6267370
Research Organizations:
Rostock Univ. (German Democratic Republic). Bereich Medizin
Country of Origin:
Germany
Language:
German
Other Identifying Numbers:
Journal ID: CODEN: FOLHA
Submitting Site:
INIS
Size:
Pages: 669-687
Announcement Date:

Journal Article:

Citation Formats

Mix, E, Jenssen, H L, Lehmitz, R, Buddenhagen, F, Hitzschke, B, and Richter, M. Effect of UVB irradiation of the blood on cellular volume adherence and phagocytosis in probands and patients with multiple sclerosis. Germany: N. p., 1988. Web.
Mix, E, Jenssen, H L, Lehmitz, R, Buddenhagen, F, Hitzschke, B, & Richter, M. Effect of UVB irradiation of the blood on cellular volume adherence and phagocytosis in probands and patients with multiple sclerosis. Germany.
Mix, E, Jenssen, H L, Lehmitz, R, Buddenhagen, F, Hitzschke, B, and Richter, M. 1988. "Effect of UVB irradiation of the blood on cellular volume adherence and phagocytosis in probands and patients with multiple sclerosis." Germany.
@misc{etde_6267370,
title = {Effect of UVB irradiation of the blood on cellular volume adherence and phagocytosis in probands and patients with multiple sclerosis}
author = {Mix, E, Jenssen, H L, Lehmitz, R, Buddenhagen, F, Hitzschke, B, and Richter, M}
abstractNote = {UVB-induced changes of blood cell properties were investigated in 12 multiple sclerosis (MS) patients and in 10 healthy volunteers. The mean cell volume (MCV) was determined by electronic sizing, the granulocyte and lymphocyte adherence was estimated in a capillary assay, and the phagocytic activity of granulocytes was measured in a test system based on the incorporation of opsonized baker's yeast. In MS patients the MCV of red cells and lymphocytes decreased rapidly within 6 UVB treatments. In contrast, the reduction of the granulocyte volume was delayed (between the 6th and 12th UVB). In the control group the mean value of the red cell and lymphocyte MCV remained rather unaffected. There was a slight rise of the granulocyte volume after the 6th UVB. The only significant change of adherence was an increase of granulocyte adherence in MS patients. Untreated patients had a significantly enhanced phagocytic activity in comparison to the control group. 6 UVB treatments induced a singificant reduction of the phagocytic activity in MS patients. However, subsequently the percentage of phagocytizing cells increased again, whereas the particle uptake per cell continued to decrease. In the control group only minor UVB-induced changes of phagocytosis were observed. The in vitro UV irradiation caused an enhanced phagocytosis in the majority of cases in both controls and MS patients. In general, under the UVB treatment all parameters examined changed in the sense of a normalisation, in that the measured values reached a new level lying between the extreme pretreatment values accompanied by a reduced standard deviation. The effect of UVB was more pronounced in MS patients when compared with normal control. This could result from an enhanced sensitivity to the influence of UVB of pathologically altered cells in MS patients. (Abstract Truncated)}
journal = {Folia Haematol. (Leipzig); (German Democratic Republic)}
volume = {115:5}
journal type = {AC}
place = {Germany}
year = {1988}
month = {Jan}
}